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Literature summary for 1.1.3.6 extracted from

  • Doukyu, N.
    Characteristics and biotechnological applications of microbial cholesterol oxidases (2009), Appl. Microbiol. Biotechnol., 83, 825-837.
    View publication on PubMed
Search for more literature for 1.1.3.6

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 markedly inhibited by AgNO3 Brevibacterium sterolicum
AgNO3 markedly inhibited by AgNO3 Corynebacterium cholesterolicum
AgNO3 markedly inhibited by AgNO3 Streptomyces sp.
AgNO3 markedly inhibited by AgNO3 Streptomyces violascens
AgNO3 markedly inhibited by AgNO3 Streptoverticillium cholesterolieum
AgNO3 markedly inhibited by AgNO3 uncultured Gammaproteobacteria bacterium
butanol the enzyme is inactivated by the addition of 50% (v/v) after incubation for 24 h at 37°C Nocardia sp.
butanol the enzyme is inactivated by the addition of 50% (v/v) of butanol after incubation for 24 h at 37°C Rhodococcus erythropolis
CuSO4 significant inhibition Streptoverticillium cholesterolieum
Dimethylsulfoxide the enzyme is inactivated by the addition of 50% (v/v) after incubation for 24 h at 37°C Nocardia sp.
Dimethylsulfoxide the enzyme is inactivated by the addition of 50% (v/v) of dimethylsulfoxide after incubation for 24 h at 37°C Rhodococcus erythropolis
ethyl acetate the enzyme is inactivated by the addition of 50% (v/v) after incubation for 24 h at 37°C Nocardia sp.
ethyl acetate the enzyme is inactivated by the addition of 50% (v/v) of ethyl acetate after incubation for 24 h at 37°C Rhodococcus erythropolis
Fe2+ markedly inhibited by Fe2+ Pseudomonas sp.
FeCl3 markedly inhibited by FeCl3 Streptomyces violascens
FeSO4 markedly inhibited by FeSO4 Streptomyces violascens
Hg2+ markedly inhibited by HgCl2 Brevibacterium sterolicum
Hg2+ markedly inhibited by HgCl2 Corynebacterium cholesterolicum
Hg2+ markedly inhibited by HgCl2 Pimelobacter simplex
Hg2+ markedly inhibited by Hg2+ Pseudomonas sp.
Hg2+ markedly inhibited by HgCl2 Streptomyces sp.
Hg2+ markedly inhibited by HgCl2 Streptomyces violascens
Hg2+ markedly inhibited by HgCl2 Streptoverticillium cholesterolieum
Hg2+ markedly inhibited by HgCl2 uncultured Gammaproteobacteria bacterium
p-chloromercuribenzoic acid the addition of p-chloromercuribenzoic acid partially reduces the activity of the enzyme Pimelobacter simplex
Zn2+ markedly inhibited by Zn2+ Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0184
-
 cholesterol pH 7.0, temperature not specified in the publication Nocardia sp.
0.0188
-
 cholesterol pH and temperature not specified in the publication Rhodococcus erythropolis
0.0262
-
 cholesterol pH and temperature not specified in the publication Chromobacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ partially activated by the addition of Mn2+ Pseudomonas sp.
Mn2+ partially activated by the addition of Mn2+ uncultured Gammaproteobacteria bacterium
additional information Ag+ scarcely influences the activity of the enzyme Chromobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
-
 Schizophyllum commune
55000
-
-
 Streptomyces sp.
55000
-
-
 Brevibacterium sterolicum
56000
-
-
 Pseudomonas sp.
56000
-
-
 Streptoverticillium cholesterolieum
57000
-
-
 Pimelobacter simplex
57000
-
-
 Corynebacterium cholesterolicum
58000
-
-
 uncultured Gammaproteobacteria bacterium
58000
-
-
 Streptomyces sp.
58000
-
-
 Chromobacterium sp.
60000
-
-
 Streptomyces fradiae
60000
-
-
 Burkholderia cepacia
63000
-
-
 Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-cholestanol + O2 Streptomyces violascens 101% activity compared to cholesterol ?
-
 ?
beta-cholestanol + O2 Brevibacterium sterolicum 13% activity compared to cholesterol ?
-
 ?
beta-cholestanol + O2 Burkholderia cepacia 69% activity compared to cholesterol ?
-
 ?
beta-cholestanol + O2 Streptomyces sp. 75% activity compared to cholesterol ?
-
 ?
beta-cholestanol + O2 Pseudomonas sp. 78% activity compared to cholesterol ?
-
 ?
beta-cholestanol + O2 uncultured Gammaproteobacteria bacterium 79% activity compared to cholesterol ?
-
 ?
beta-cholestanol + O2 Chromobacterium sp. 95% activity compared to cholesterol ?
-
 ?
beta-cholestanol + O2 Streptoverticillium cholesterolieum 96% activity compared to cholesterol ?
-
 ?
beta-cholestanol + O2 Pseudomonas sp. COX629 78% activity compared to cholesterol ?
-
 ?
beta-cholestanol + O2 uncultured Gammaproteobacteria bacterium Y-134 79% activity compared to cholesterol ?
-
 ?
beta-sitosterol + O2 Brevibacterium sterolicum 20% activity compared to cholesterol stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 uncultured Gammaproteobacteria bacterium 29% activity compared to cholesterol stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 Streptoverticillium cholesterolieum 39% activity compared to cholesterol stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 Corynebacterium cholesterolicum 50% activity compared to cholesterol stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 Streptomyces violascens 64% activity compared to cholesterol stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 Streptomyces sp. 72% activity compared to cholesterol stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 Chromobacterium sp. 80% activity compared to cholesterol stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 Burkholderia cepacia 84% activity compared to cholesterol stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 Burkholderia cepacia ST-200 84% activity compared to cholesterol stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 uncultured Gammaproteobacteria bacterium Y-134 29% activity compared to cholesterol stigmast-5-en-3-one + H2O2
-
 ?
beta-stigmasterol + O2 Brevibacterium sterolicum 10% activity compared to cholesterol stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 Corynebacterium cholesterolicum 12% activity compared to cholesterol stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 Streptoverticillium cholesterolieum 17% activity compared to cholesterol stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 uncultured Gammaproteobacteria bacterium 32% activity compared to cholesterol stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 Chromobacterium sp. 36% activity compared to cholesterol stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 Streptomyces sp. 37% activity compared to cholesterol stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 Pseudomonas sp. 40% activity compared to cholesterol stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 Pimelobacter simplex 47% activity compared to cholesterol stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 Streptomyces violascens 50% activity compared to cholesterol stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 Burkholderia cepacia 59% activity compared to cholesterol stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 Pseudomonas sp. COX629 40% activity compared to cholesterol stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 uncultured Gammaproteobacteria bacterium Y-134 32% activity compared to cholesterol stigmasta-5,22-dien-3-one + H2O2
-
 ?
cholesterol + O2 Pseudomonas aeruginosa
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Arthrobacter sp.
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Streptomyces fradiae
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Nocardia rhodochrous
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Burkholderia pseudomallei
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Rhodococcus equi
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Streptomyces avermitilis
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Streptomyces griseus
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Streptomyces natalensis
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Streptomyces sp.
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Corynebacterium urealyticum
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Nocardia farcinica
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Mycobacterium leprae
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Mycobacterium tuberculosis
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Streptomyces coelicolor
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Rhodococcus erythropolis
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Burkholderia thailandensis
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Paraburkholderia xenovorans
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Pseudomonas sp. 100% activity cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Pimelobacter simplex 100% activity cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Nocardia sp. 100% activity cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Schizophyllum commune 100% activity cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Streptomyces violascens 100% activity cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Corynebacterium cholesterolicum 100% activity cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 uncultured Gammaproteobacteria bacterium 100% activity cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Streptomyces sp. 100% activity cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Burkholderia cepacia 100% activity cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Chromobacterium sp. 100% activity cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Streptoverticillium cholesterolieum 100% activity cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Brevibacterium sterolicum 100% activity cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Burkholderia cepacia ST-200 100% activity cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Rhodococcus equi 103S
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Nocardia farcinica IFM 10152
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Pseudomonas sp. COX629 100% activity cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Corynebacterium urealyticum DSM 7109
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Mycobacterium tuberculosis H37Rv
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 Arthrobacter sp. IM79
-
 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 uncultured Gammaproteobacteria bacterium Y-134 100% activity cholest-4-en-3-one + H2O2
-
 ?
dehydroepiandrosterone + O2 Burkholderia cepacia 16% activity compared to cholesterol androst-5-en-3,17-dione + H2O2
-
 ?
dehydroepiandrosterone + O2 Streptomyces sp. 24% activity compared to cholesterol androst-5-en-3,17-dione + H2O2
-
 ?
dehydroepiandrosterone + O2 Chromobacterium sp. 27% activity compared to cholesterol androst-5-en-3,17-dione + H2O2
-
 ?
dehydroepiandrosterone + O2 Schizophyllum commune 28% activity compared to cholesterol androst-5-en-3,17-dione + H2O2
-
 ?
dehydroepiandrosterone + O2 Streptomyces violascens 37% activity compared to cholesterol androst-5-en-3,17-dione + H2O2
-
 ?
dehydroepiandrosterone + O2 Brevibacterium sterolicum 41% activity compared to cholesterol androst-5-en-3,17-dione + H2O2
-
 ?
dehydroepiandrosterone + O2 Streptoverticillium cholesterolieum 48% activity compared to cholesterol androst-5-en-3,17-dione + H2O2
-
 ?
dehydroepiandrosterone + O2 Corynebacterium cholesterolicum 5% activity compared to cholesterol androst-5-en-3,17-dione + H2O2
-
 ?
dehydroepiandrosterone + O2 Burkholderia cepacia ST-200 16% activity compared to cholesterol androst-5-en-3,17-dione + H2O2
-
 ?
epiandrosterone + O2 Nocardia sp. 1% activity compared to cholesterol ?
-
 ?
epiandrosterone + O2 Burkholderia cepacia 10% activity compared to cholesterol ?
-
 ?
epiandrosterone + O2 Chromobacterium sp. 15% activity compared to cholesterol ?
-
 ?
ergosterol + O2 Streptomyces sp. 12% activity compared to cholesterol dehydroergosterol + H2O2
-
 ?
ergosterol + O2 Chromobacterium sp. 12% activity compared to cholesterol dehydroergosterol + H2O2
-
 ?
ergosterol + O2 Streptomyces violascens 14% activity compared to cholesterol dehydroergosterol + H2O2
-
 ?
ergosterol + O2 Pimelobacter simplex 17% activity compared to cholesterol dehydroergosterol + H2O2
-
 ?
ergosterol + O2 Burkholderia cepacia 20% activity compared to cholesterol dehydroergosterol + H2O2
-
 ?
ergosterol + O2 Corynebacterium cholesterolicum 4% activity compared to cholesterol dehydroergosterol + H2O2
-
 ?
ergosterol + O2 Streptoverticillium cholesterolieum 5% activity compared to cholesterol dehydroergosterol + H2O2
-
 ?
ergosterol + O2 Pseudomonas sp. 51% activity compared to cholesterol dehydroergosterol + H2O2
-
 ?
ergosterol + O2 Burkholderia cepacia ST-200 20% activity compared to cholesterol dehydroergosterol + H2O2
-
 ?
ergosterol + O2 Pseudomonas sp. COX629 51% activity compared to cholesterol dehydroergosterol + H2O2
-
 ?
additional information Pseudomonas sp. no activity with dehydroepiandrosterone ?
-
 ?
additional information Schizophyllum commune no activity with ergosterol ?
-
 ?
additional information Brevibacterium sterolicum no activity with ergosterol ?
-
 ?
additional information uncultured Gammaproteobacteria bacterium no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone ?
-
 ?
additional information Rhodococcus erythropolis the oxidation of the allylic alcohols by cholesterol oxidase from Rhodococcus erythropolis is stereo- and enantioselective. The enzyme oxidizes (S)-alcohol at a higher rate than (R)-alcohol ?
-
 ?
additional information Pseudomonas sp. COX629 no activity with dehydroepiandrosterone ?
-
 ?
additional information uncultured Gammaproteobacteria bacterium Y-134 no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone ?
-
 ?
pregnenolone + O2 Schizophyllum commune 20% activity compared to cholesterol 5-pregnen-3,20-dione + H2O2
-
 ?
pregnenolone + O2 Brevibacterium sterolicum 22% activity compared to cholesterol 5-pregnen-3,20-dione + H2O2
-
 ?
pregnenolone + O2 Burkholderia cepacia 32% activity compared to cholesterol 5-pregnen-3,20-dione + H2O2
-
 ?
pregnenolone + O2 Streptoverticillium cholesterolieum 37% activity compared to cholesterol 5-pregnen-3,20-dione + H2O2
-
 ?
pregnenolone + O2 Corynebacterium cholesterolicum 38% activity compared to cholesterol 5-pregnen-3,20-dione + H2O2
-
 ?
pregnenolone + O2 Streptomyces violascens 42% activity compared to cholesterol 5-pregnen-3,20-dione + H2O2
-
 ?
pregnenolone + O2 Nocardia sp. 6% activity compared to cholesterol 5-pregnen-3,20-dione + H2O2
-
 ?
pregnenolone + O2 Chromobacterium sp. 95% activity compared to cholesterol 5-pregnen-3,20-dione + H2O2
-
 ?
pregnenolone + O2 Streptomyces sp. 99% activity compared to cholesterol 5-pregnen-3,20-dione + H2O2
-
 ?
pregnenolone + O2 Burkholderia cepacia ST-200 32% activity compared to cholesterol 5-pregnen-3,20-dione + H2O2
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone the enzyme is unstable in 50% (v/v) acetone Chromobacterium sp.
Acetone the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C Chromobacterium sp.
Butanol the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C Chromobacterium sp.
DMSO the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C Chromobacterium sp.
Emal 20CM the enzyme is relatively tolerant to treatment with Emal 20CM even at 60°C Chromobacterium sp.
Ethanol the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C Chromobacterium sp.
Ethyl acetate the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C Chromobacterium sp.
isopropanol the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C Chromobacterium sp.
Methanol the enzyme remains stable after incubation in 50% (v/v) for 24 h at 37°C Chromobacterium sp.
propan-2-ol the activity of the enzyme is rapidly inactivated in the presence of 30% (v/v) propan-2-ol at 25°C Brevibacterium sterolicum
SDS the enzyme is relatively tolerant to treatment with SDS after incubation for 1 h at 30°C Chromobacterium sp.
sodium cholate the enzyme is stable at 50% (v/v) sodium cholate after incubation for 1 h at 30°C Nocardia sp.
sodium cholate the enzyme is stable in sodium cholate after incubation for 1 h at 30°C Rhodococcus erythropolis
sodium cholate the enzyme retains more than 80% of its original activity in 0.5% (v/v) sodium cholate after incubation for 1 h at 60°C uncultured Gammaproteobacteria bacterium
sodium dodecyl sarcosinate the enzyme is relatively tolerant to treatment with sodium dodecyl sarcosinate even at 60°C Chromobacterium sp.
sodium laurylbenzenesulfonate the enzyme is relatively tolerant to treatment with sodium laurylbenzenesulfonate after incubation for 1 h at 30°C Chromobacterium sp.
sodium polyoxyethylene alkyl ether sulfate the enzyme is stable at 50% (v/v) sodium polyoxyethylene alkyl ether sulfate after incubation for 1 h at 30°C Nocardia sp.
sodium polyoxyethylene alkyl ether sulfate the enzyme is stable in sodium polyoxyethylene alkyl ether sulfate after incubation for 1 h at 30°C Rhodococcus erythropolis
Triton X-100 the enzyme is stable at 50% (v/v) Triton X-100 after incubation for 1 h at 30°C Nocardia sp.
Triton X-100 the enzyme is stable in Triton X-100 after incubation for 1 h at 30°C Rhodococcus erythropolis
Triton X-405 the enzyme is stable at 50% (v/v) Triton X-405 after incubation for 1 h at 30°C Nocardia sp.
Triton X-405 the enzyme is stable in Triton X-405 after incubation for 1 h at 30°C Rhodococcus erythropolis
Triton X-405 the enzyme retains more than 80% of its original activity in 0.5% (v/v) Triton X-405 after incubation for 1 h at 60°C uncultured Gammaproteobacteria bacterium
Tween 20 the enzyme is stable at 50% (v/v) Tween 20 after incubation for 1 h at 30°C Nocardia sp.
Tween 20 the enzyme is stable in Tween 20 after incubation for 1 h at 30°C Rhodococcus erythropolis

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. Q56DL0
-
-
Arthrobacter sp. IM79
-
-
-
Brevibacterium sterolicum P22637
-
-
Burkholderia cepacia Q93RE1
-
-
Burkholderia cepacia ST-200 Q93RE1
-
-
Burkholderia pseudomallei
-
-
-
Burkholderia thailandensis Q2T0X3
-
-
Chromobacterium sp. B5MGF8
-
-
Corynebacterium cholesterolicum
-
-
-
Corynebacterium urealyticum B1VGH1
-
-
Corynebacterium urealyticum DSM 7109 B1VGH1
-
-
Mycobacterium leprae Q59530
-
-
Mycobacterium tuberculosis P9WMV9
-
-
Mycobacterium tuberculosis H37Rv P9WMV9
-
-
Nocardia farcinica Q5YRJ2
-
-
Nocardia farcinica IFM 10152 Q5YRJ2
-
-
Nocardia rhodochrous
-
-
-
Nocardia sp.
-
-
-
Paraburkholderia xenovorans Q13UN0
-
-
Pimelobacter simplex
-
-
-
Pseudomonas aeruginosa
-
-
-
Pseudomonas sp.
-
-
-
Pseudomonas sp. COX629
-
-
-
Rhodococcus equi A0A3S5YA89
-
-
Rhodococcus equi 103S A0A3S5YA89
-
-
Rhodococcus erythropolis A9QAE7
-
-
Schizophyllum commune
-
-
-
Streptomyces avermitilis Q93H76
-
-
Streptomyces coelicolor Q9L0H6
-
-
Streptomyces fradiae
-
-
-
Streptomyces griseus A7RA76
-
-
Streptomyces natalensis Q9EW96
-
-
Streptomyces sp. P12676
-
-
Streptomyces sp. Q55020
-
-
Streptomyces violascens
-
-
-
Streptoverticillium cholesterolieum
-
-
-
uncultured Gammaproteobacteria bacterium
-
-
-
uncultured Gammaproteobacteria bacterium Y-134
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-cholestanol + O2 101% activity compared to cholesterol Streptomyces violascens ?
-
 ?
beta-cholestanol + O2 13% activity compared to cholesterol Brevibacterium sterolicum ?
-
 ?
beta-cholestanol + O2 69% activity compared to cholesterol Burkholderia cepacia ?
-
 ?
beta-cholestanol + O2 75% activity compared to cholesterol Streptomyces sp. ?
-
 ?
beta-cholestanol + O2 78% activity compared to cholesterol Pseudomonas sp. ?
-
 ?
beta-cholestanol + O2 79% activity compared to cholesterol uncultured Gammaproteobacteria bacterium ?
-
 ?
beta-cholestanol + O2 95% activity compared to cholesterol Chromobacterium sp. ?
-
 ?
beta-cholestanol + O2 96% activity compared to cholesterol Streptoverticillium cholesterolieum ?
-
 ?
beta-cholestanol + O2 78% activity compared to cholesterol Pseudomonas sp. COX629 ?
-
 ?
beta-cholestanol + O2 79% activity compared to cholesterol uncultured Gammaproteobacteria bacterium Y-134 ?
-
 ?
beta-sitosterol + O2 20% activity compared to cholesterol Brevibacterium sterolicum stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 29% activity compared to cholesterol uncultured Gammaproteobacteria bacterium stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 39% activity compared to cholesterol Streptoverticillium cholesterolieum stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 50% activity compared to cholesterol Corynebacterium cholesterolicum stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 64% activity compared to cholesterol Streptomyces violascens stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 72% activity compared to cholesterol Streptomyces sp. stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 80% activity compared to cholesterol Chromobacterium sp. stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 84% activity compared to cholesterol Burkholderia cepacia stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 84% activity compared to cholesterol Burkholderia cepacia ST-200 stigmast-5-en-3-one + H2O2
-
 ?
beta-sitosterol + O2 29% activity compared to cholesterol uncultured Gammaproteobacteria bacterium Y-134 stigmast-5-en-3-one + H2O2
-
 ?
beta-stigmasterol + O2 10% activity compared to cholesterol Brevibacterium sterolicum stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 12% activity compared to cholesterol Corynebacterium cholesterolicum stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 17% activity compared to cholesterol Streptoverticillium cholesterolieum stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 32% activity compared to cholesterol uncultured Gammaproteobacteria bacterium stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 36% activity compared to cholesterol Chromobacterium sp. stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 37% activity compared to cholesterol Streptomyces sp. stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 40% activity compared to cholesterol Pseudomonas sp. stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 47% activity compared to cholesterol Pimelobacter simplex stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 50% activity compared to cholesterol Streptomyces violascens stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 59% activity compared to cholesterol Burkholderia cepacia stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 40% activity compared to cholesterol Pseudomonas sp. COX629 stigmasta-5,22-dien-3-one + H2O2
-
 ?
beta-stigmasterol + O2 32% activity compared to cholesterol uncultured Gammaproteobacteria bacterium Y-134 stigmasta-5,22-dien-3-one + H2O2
-
 ?
cholesterol + O2
-
 Pseudomonas aeruginosa cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Arthrobacter sp. cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Streptomyces fradiae cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Nocardia rhodochrous cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Burkholderia pseudomallei cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Rhodococcus equi cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Streptomyces avermitilis cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Streptomyces griseus cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Streptomyces natalensis cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Streptomyces sp. cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Corynebacterium urealyticum cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Nocardia farcinica cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Mycobacterium leprae cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Mycobacterium tuberculosis cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Streptomyces coelicolor cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Rhodococcus erythropolis cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Burkholderia thailandensis cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Paraburkholderia xenovorans cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 100% activity Pseudomonas sp. cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 100% activity Pimelobacter simplex cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 100% activity Nocardia sp. cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 100% activity Schizophyllum commune cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 100% activity Streptomyces violascens cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 100% activity Corynebacterium cholesterolicum cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 100% activity uncultured Gammaproteobacteria bacterium cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 100% activity Streptomyces sp. cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 100% activity Burkholderia cepacia cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 100% activity Chromobacterium sp. cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 100% activity Streptoverticillium cholesterolieum cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 100% activity Brevibacterium sterolicum cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 100% activity Burkholderia cepacia ST-200 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Rhodococcus equi 103S cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Nocardia farcinica IFM 10152 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 100% activity Pseudomonas sp. COX629 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Corynebacterium urealyticum DSM 7109 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Mycobacterium tuberculosis H37Rv cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2
-
 Arthrobacter sp. IM79 cholest-4-en-3-one + H2O2
-
 ?
cholesterol + O2 100% activity uncultured Gammaproteobacteria bacterium Y-134 cholest-4-en-3-one + H2O2
-
 ?
dehydroepiandrosterone + O2 16% activity compared to cholesterol Burkholderia cepacia androst-5-en-3,17-dione + H2O2
-
 ?
dehydroepiandrosterone + O2 24% activity compared to cholesterol Streptomyces sp. androst-5-en-3,17-dione + H2O2
-
 ?
dehydroepiandrosterone + O2 27% activity compared to cholesterol Chromobacterium sp. androst-5-en-3,17-dione + H2O2
-
 ?
dehydroepiandrosterone + O2 28% activity compared to cholesterol Schizophyllum commune androst-5-en-3,17-dione + H2O2
-
 ?
dehydroepiandrosterone + O2 37% activity compared to cholesterol Streptomyces violascens androst-5-en-3,17-dione + H2O2
-
 ?
dehydroepiandrosterone + O2 41% activity compared to cholesterol Brevibacterium sterolicum androst-5-en-3,17-dione + H2O2
-
 ?
dehydroepiandrosterone + O2 48% activity compared to cholesterol Streptoverticillium cholesterolieum androst-5-en-3,17-dione + H2O2
-
 ?
dehydroepiandrosterone + O2 5% activity compared to cholesterol Corynebacterium cholesterolicum androst-5-en-3,17-dione + H2O2
-
 ?
dehydroepiandrosterone + O2 16% activity compared to cholesterol Burkholderia cepacia ST-200 androst-5-en-3,17-dione + H2O2
-
 ?
epiandrosterone + O2 1% activity compared to cholesterol Nocardia sp. ?
-
 ?
epiandrosterone + O2 10% activity compared to cholesterol Burkholderia cepacia ?
-
 ?
epiandrosterone + O2 15% activity compared to cholesterol Chromobacterium sp. ?
-
 ?
ergosterol + O2 12% activity compared to cholesterol Streptomyces sp. dehydroergosterol + H2O2
-
 ?
ergosterol + O2 12% activity compared to cholesterol Chromobacterium sp. dehydroergosterol + H2O2
-
 ?
ergosterol + O2 14% activity compared to cholesterol Streptomyces violascens dehydroergosterol + H2O2
-
 ?
ergosterol + O2 17% activity compared to cholesterol Pimelobacter simplex dehydroergosterol + H2O2
-
 ?
ergosterol + O2 20% activity compared to cholesterol Burkholderia cepacia dehydroergosterol + H2O2
-
 ?
ergosterol + O2 4% activity compared to cholesterol Corynebacterium cholesterolicum dehydroergosterol + H2O2
-
 ?
ergosterol + O2 5% activity compared to cholesterol Streptoverticillium cholesterolieum dehydroergosterol + H2O2
-
 ?
ergosterol + O2 51% activity compared to cholesterol Pseudomonas sp. dehydroergosterol + H2O2
-
 ?
ergosterol + O2 20% activity compared to cholesterol Burkholderia cepacia ST-200 dehydroergosterol + H2O2
-
 ?
ergosterol + O2 51% activity compared to cholesterol Pseudomonas sp. COX629 dehydroergosterol + H2O2
-
 ?
additional information no activity with dehydroepiandrosterone Pseudomonas sp. ?
-
 ?
additional information no activity with ergosterol Schizophyllum commune ?
-
 ?
additional information no activity with ergosterol Brevibacterium sterolicum ?
-
 ?
additional information no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone uncultured Gammaproteobacteria bacterium ?
-
 ?
additional information the oxidation of the allylic alcohols by cholesterol oxidase from Rhodococcus erythropolis is stereo- and enantioselective. The enzyme oxidizes (S)-alcohol at a higher rate than (R)-alcohol Rhodococcus erythropolis ?
-
 ?
additional information no activity with dehydroepiandrosterone Pseudomonas sp. COX629 ?
-
 ?
additional information no activity with ergosterol, pregnenolone, dehydroepiandrosterone, and epiandosterone uncultured Gammaproteobacteria bacterium Y-134 ?
-
 ?
pregnenolone + O2 20% activity compared to cholesterol Schizophyllum commune 5-pregnen-3,20-dione + H2O2
-
 ?
pregnenolone + O2 22% activity compared to cholesterol Brevibacterium sterolicum 5-pregnen-3,20-dione + H2O2
-
 ?
pregnenolone + O2 32% activity compared to cholesterol Burkholderia cepacia 5-pregnen-3,20-dione + H2O2
-
 ?
pregnenolone + O2 37% activity compared to cholesterol Streptoverticillium cholesterolieum 5-pregnen-3,20-dione + H2O2
-
 ?
pregnenolone + O2 38% activity compared to cholesterol Corynebacterium cholesterolicum 5-pregnen-3,20-dione + H2O2
-
 ?
pregnenolone + O2 42% activity compared to cholesterol Streptomyces violascens 5-pregnen-3,20-dione + H2O2
-
 ?
pregnenolone + O2 6% activity compared to cholesterol Nocardia sp. 5-pregnen-3,20-dione + H2O2
-
 ?
pregnenolone + O2 95% activity compared to cholesterol Chromobacterium sp. 5-pregnen-3,20-dione + H2O2
-
 ?
pregnenolone + O2 99% activity compared to cholesterol Streptomyces sp. 5-pregnen-3,20-dione + H2O2
-
 ?
pregnenolone + O2 32% activity compared to cholesterol Burkholderia cepacia ST-200 5-pregnen-3,20-dione + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
CHOD
-
 Mycobacterium tuberculosis
PimE
-
 Streptomyces natalensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Nocardia rhodochrous
40
-
-
 Rhodococcus equi
40 42
-
 Corynebacterium cholesterolicum
40 50
-
 Arthrobacter sp.
45 50
-
 Streptomyces sp.
50
-
-
 Pimelobacter simplex
50
-
-
 Streptomyces violascens
50
-
-
 Brevibacterium sterolicum
60
-
-
 Burkholderia cepacia
65
-
-
 Chromobacterium sp.
70
-
-
 Streptomyces fradiae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 96% activity after 30 min at 50°C, 6% activity after 30 min at 60°C Corynebacterium cholesterolicum
55
-
 less than 50% activity after 15 min at 55°C Streptomyces sp.
60 85 the enzyme retains more than 90% of its original activity after incubation for 240 min at 60°C, 90% activity after 1 h at 80°C, and 80% activity after 30 min at 85°C Chromobacterium sp.
70
-
 10% activity after 1 h at 70°C Streptomyces fradiae
70 80 70% activity after 30 min at 70°C, complete loss of activity after 30 min at 80°C Pseudomonas sp.
70 80 70% residual activity after 30 min at 70°C, complete loss of activity after 30 min at 80°C Burkholderia cepacia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Schizophyllum commune
6.5
-
-
 uncultured Gammaproteobacteria bacterium
6.5 7
-
 Streptomyces sp.
6.8 8
-
 Burkholderia cepacia
7 7.5
-
 Corynebacterium cholesterolicum
7 7.5
-
 Chromobacterium sp.
7 7.5
-
 Streptoverticillium cholesterolieum
7
-
-
 Pseudomonas sp.
7
-
-
 Nocardia sp.
7
-
-
 Streptomyces fradiae
7
-
-
 Nocardia rhodochrous
7
-
-
 Streptomyces violascens
7
-
-
 Streptomyces sp.
7.5
-
-
 Pimelobacter simplex
7.5
-
-
 Brevibacterium sterolicum
7.5 8.5
-
 Arthrobacter sp.
8
-
-
 Rhodococcus equi

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 11 30°C, 1 h Chromobacterium sp.
4 8 25°C, 20 h Streptomyces sp.
4 10 37°C, 30 min Brevibacterium sterolicum
4 10 4°C, 4 h Streptomyces fradiae
4 11 30°C, 1 h Burkholderia cepacia
4 11 60°C, 1 min Pseudomonas sp.
4 12.5 22°C, 1 h Streptoverticillium cholesterolieum
5 7.5 60°C, 1 h uncultured Gammaproteobacteria bacterium
5 9 30°C, 1 h Corynebacterium cholesterolicum

Cofactor

Cofactor Comment Organism Structure
FAD dependent on Pseudomonas aeruginosa
FAD dependent on Pseudomonas sp.
FAD dependent on Pimelobacter simplex
FAD dependent on Arthrobacter sp.
FAD dependent on Nocardia sp.
FAD dependent on Streptomyces fradiae
FAD dependent on Nocardia rhodochrous
FAD dependent on Schizophyllum commune
FAD dependent on Streptomyces violascens
FAD dependent on Corynebacterium cholesterolicum
FAD dependent on Burkholderia pseudomallei
FAD dependent on uncultured Gammaproteobacteria bacterium
FAD dependent on Streptomyces sp.
FAD dependent on Rhodococcus equi
FAD dependent on Streptomyces avermitilis
FAD dependent on Streptomyces griseus
FAD dependent on Streptomyces natalensis
FAD dependent on Corynebacterium urealyticum
FAD dependent on Nocardia farcinica
FAD dependent on Mycobacterium leprae
FAD dependent on Mycobacterium tuberculosis
FAD dependent on Streptomyces coelicolor
FAD dependent on Rhodococcus erythropolis
FAD dependent on Burkholderia cepacia
FAD dependent on Burkholderia thailandensis
FAD dependent on Paraburkholderia xenovorans
FAD dependent on Chromobacterium sp.
FAD dependent on Streptoverticillium cholesterolieum
FAD dependent on Brevibacterium sterolicum

General Information

General Information Comment Organism
metabolism PimE is a key enzyme in the biosynthesis of the polyene macrolide pimaricin Streptomyces natalensis
physiological function ChoD plays a significant role in the pathogenesis of Mycobacterium tuberculosis Mycobacterium tuberculosis