General Stability | Organism |
---|---|
the ChOx-immobilized silk mat maintains its initial activity till the fourth successive measurement and retains about 50% of its initial activity at the end of 25 measurements | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2123 | - |
cholesterol | apparent value, free enzyme, in 50 mM potassium phosphate buffer, pH 7.5, 30°C | Pseudomonas fluorescens | |
0.2599 | - |
cholesterol | apparent value, silk mat-immobilized enzyme, in 50 mM potassium phosphate buffer, pH 7.5, 30°C | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Storage Stability | Organism |
---|---|
4°C, ChOx-immobilized silk mat, when the measurements are made at 3 days interval the immobilized ChOx can retain about 50% of its original activity after 45 days of storage and when the same carried out with 7 days interval, about 70% of its original activity is retained till 60 days | Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Pseudomonas fluorescens | cholest-4-en-3-one + H2O2 | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
ChOx | - |
Pseudomonas fluorescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
silk mat-immobilized enzyme | Pseudomonas fluorescens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | the oxygen depletion rate is increased with increasing temperature from 25°C to 40°C but it decreases sharply as the temperature increases further to 45°C | Pseudomonas fluorescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
silk mat-immobilized enzyme | Pseudomonas fluorescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 7.5 | the activity increases with increasing pH value from 4.0 to 7.5 and then declines as pH increases further | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas fluorescens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
cholesterol | apparent value, silk mat-immobilized enzyme, in 50 mM potassium phosphate buffer, pH 7.5, 30°C | Pseudomonas fluorescens |