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Literature summary for 1.1.3.6 extracted from

  • Srisawasdi, P.; Jearanaikoon, P.; Wetprasit, N.; Sriwanthana, B.; Kroll, M.H.; Lolekha, P.H.
    Application of Streptomyces and Brevibacterium cholesterol oxidase for total serum cholesterol assay by the enzymatic kinetic method (2006), Clin. Chim. Acta, 372, 103-111.
    View publication on PubMed

Application

Application Comment Organism
diagnostics the enzyme is used for a kinetic cholesterol assay for determination of cholesterol content in human serum, overview, the enzyme from Streptomyces is more effective than the enzyme from Brevibacterium Streptomyces sp.
diagnostics the enzyme is used for a kinetic cholesterol assay for determination of cholesterol content in human serum, overview, the enzyme from Streptomyces is more effective than the enzyme from Brevibacterium Brevibacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics, modelling Streptomyces sp.
additional information
-
additional information kinetics, modelling Brevibacterium sp.

Organism

Organism UniProt Comment Textmining
Brevibacterium sp.
-
-
-
Streptomyces sp.
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Streptomyces sp.
additional information
-
-
Brevibacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
Streptomyces sp. cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Brevibacterium sp. cholest-4-en-3-one + H2O2
-
?