General Stability | Organism |
---|---|
stable in nonionic detergents over a wide range. More than 90% of activity remains after 60 min at 60°C, at concentrations of 0.1% and 0.5% of emulgen A-60, Triton X-100, Triton X-405 or sodium cholate | gamma proteobacterium Y-134 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-pyridylhydroxymethane sulfate | 1 mM, 30 min at 4°C, 32% inhibition | gamma proteobacterium Y-134 | |
8-hydroxyquinoline | 1 mM, 30 min at 4°C, 20% inhibition | gamma proteobacterium Y-134 | |
CoCl2 | 1 mM, 30 min at 4°C, 21% inhibition | gamma proteobacterium Y-134 | |
CuCl2 | 1 mM, 30 min at 4°C, 29% inhibition | gamma proteobacterium Y-134 | |
FAD | 1 mM, 30 min at 4°C, 21% inhibition | gamma proteobacterium Y-134 | |
FMN | 1 mM, 30 min at 4°C, 21% inhibition | gamma proteobacterium Y-134 | |
iodoacetic acid | 1 mM, 30 min at 4°C, 15% inhibition | gamma proteobacterium Y-134 | |
MgCl2 | 1 mM, 30 min at 4°C, 23% inhibition | gamma proteobacterium Y-134 | |
MnCl2 | 1 mM, 30 min at 4°C, 35% inhibition | gamma proteobacterium Y-134 | |
NEM | 1 mM, 30 min at 4°C, 24% inhibition | gamma proteobacterium Y-134 | |
o-phenanthroline | 1 mM, 30 min at 4°C, 26% inhibition | gamma proteobacterium Y-134 | |
potassium cyanide | 1 mM, 30 min at 4°C, 15% inhibition | gamma proteobacterium Y-134 | |
riboflavin | 1 mM, 30 min at 4°C, 20% inhibition | gamma proteobacterium Y-134 | |
rotenone | 1 mM, 30 min at 4°C, 12% inhibition | gamma proteobacterium Y-134 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
cholesterol | pH 6.0 or pH 7.0 | gamma proteobacterium Y-134 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
gel filtration | gamma proteobacterium Y-134 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
gamma proteobacterium Y-134 | - |
uncultured | - |
Purification (Comment) | Organism |
---|---|
enzyme CHO-U | gamma proteobacterium Y-134 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
39.6 | - |
- |
gamma proteobacterium Y-134 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-dehydrocholesterol + O2 | 26% of the activity with cholesterol | gamma proteobacterium Y-134 | cholest-4,7-dien-3-one + H2O2 | - |
? | |
beta-sitosterol + O2 | 19% of the activity with cholesterol | gamma proteobacterium Y-134 | stigmast-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
gamma proteobacterium Y-134 | cholest-4-en-3-one + H2O2 | - |
? | |
dihydrocholesterol + O2 | 22% of the activity with cholesterol | gamma proteobacterium Y-134 | 5alpha-cholestan-3-one + H2O2 | - |
? | |
additional information | less than 3% of the activity with cholesterol: ergosterol and epiandrosterone. No activity: dehydroepiandrosterone, pregnenolone | gamma proteobacterium Y-134 | ? | - |
? | |
stigmastanol + O2 | 7% of the activity with cholesterol | gamma proteobacterium Y-134 | stigmastanone + H2O2 | - |
? | |
stigmasterol + O2 | 15% of the activity with cholesterol | gamma proteobacterium Y-134 | stigmasta-5,22-dien-3-one + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 58000, SDS-PAGE | gamma proteobacterium Y-134 |
Synonyms | Comment | Organism |
---|---|---|
CHO-U | - |
gamma proteobacterium Y-134 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH 6.0, 7 h, approximately 65% loss of activity of CHO-U | gamma proteobacterium Y-134 |
70 | - |
pH 6.0, 1 h, 40% loss of activity of CHO-U | gamma proteobacterium Y-134 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6 | - |
gamma proteobacterium Y-134 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 6.5 | pH 5.0: about 90% of maximal activity, pH 6.5: about 60% of maximal activity, pH 7.0: about 20% of maximal activity | gamma proteobacterium Y-134 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | 60 min, more than 80% of the CHO-U activity remains | gamma proteobacterium Y-134 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
gamma proteobacterium Y-134 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
gamma proteobacterium Y-134 | isoelectric fucusing, pH-gradient 3.5-10.0 | - |
7 |