Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Benzene | strain ST-200, 3fold increase in activity, 1 ml solvent added to 2 ml 50 mM phosphate buffer, pH 7.0 | Pseudomonas sp. | |
diphenylmethane | strain ST-200, 3.1fold increase in activity, 1 ml solvent added to 2 ml 50 mM phosphate buffer, pH 7.0 | Pseudomonas sp. | |
p-xylene | strain ST-200, 3.4fold increase in activity, 1 ml solvent added to 2 ml 50 mM phosphate buffer, pH 7.0 | Pseudomonas sp. | |
propylbenzene | strain ST-200, 3.2fold increase in activity, 1 ml solvent added to 2 ml 50 mM phosphate buffer, pH 7.0 | Pseudomonas sp. | |
Toluene | strain ST-200, 3.5fold increase in activity, 1 ml solvent added to 2 ml 50 mM phosphate buffer, pH 7.0 | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
cholesterol | strain ST-200 | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Pseudomonas sp. | - |
cholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | Pseudomonas sp. ST-200 | - |
cholest-5-en-3-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
ST-200 | - |
Pseudomonas sp. ST-200 | - |
ST-200 | - |
Purification (Comment) | Organism |
---|---|
strain ST-200, ammonium sulfate, DEAE-cellulose, butyl-toyopearl, Sephadex G-100 | Pseudomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.2 | - |
strain ST-200 | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Pseudomonas sp. | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Pseudomonas sp. ST-200 | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Pseudomonas sp. | cholest-5-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | - |
Pseudomonas sp. ST-200 | cholest-5-en-3-one + H2O2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
strain ST-200 | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 50 | strain ST-200, 27% activity lost after incubation at 60°C, complete loss of activity at 70°C | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 8 | strain ST-200 | Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | strain ST-200 | Pseudomonas sp. |