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Literature summary for 1.1.3.6 extracted from

  • Uwajima, T.; Yagi, H.; Nakamura, S.; Terada, O.
    Isolation and crystallization of extracellular 3beta-hydroxysteroid oxidase of Brevibacterium sterolicum nov. Sp. (1973), Agric. Biol. Chem., 37, 2345-2350.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
rod shaped crystals with bright yellow color Brevibacterium sterolicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholesterol + O2 Brevibacterium sterolicum
-
cholest-5-en-3-one + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Brevibacterium sterolicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Brevibacterium sterolicum

Reaction

Reaction Comment Organism Reaction ID
cholesterol + O2 = cholest-5-en-3-one + H2O2 bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid Brevibacterium sterolicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17
-
-
Brevibacterium sterolicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholesterol + O2
-
Brevibacterium sterolicum cholest-4-en-3-one + H2O2
-
?
cholesterol + O2
-
Brevibacterium sterolicum cholest-5-en-3-one + H2O2
-
?

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Brevibacterium sterolicum