Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | stimulates at concentration up to 0.2% | Nocardia rhodochrous |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cholest-4-en-3-one | - |
Nocardia rhodochrous | |
HgCl2 | 10 mM, complete inhibition | Nocardia rhodochrous |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | Nocardia rhodochrous | first step in cholesterol degradation | cholest-5-en-3-one + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardia rhodochrous | - |
3 forms | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | 3 enzyme forms differ chiefly in the presence or absence of hydrophobic anchor region connected by a trypsin-sensitive region, no phospholipids are extracted with the enzyme | Nocardia rhodochrous |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cholesterol + O2 = cholest-5-en-3-one + H2O2 | bifunctional enzyme, catalyzes both the oxidation of DELTA5-ene-3beta hydroxysteroids with a trans A-B ring junction to the corresponding DELTA5-3-ketosteroid with the reduction of oxygen to hydrogen peroxide, and the isomerization to the DELTA4-3-ketosteroid | Nocardia rhodochrous |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.045 | 0.069 | substrate cholesterol | Nocardia rhodochrous |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + O2 | - |
Nocardia rhodochrous | cholest-4-en-3-one + H2O2 | - |
? | |
cholesterol + O2 | first step in cholesterol degradation | Nocardia rhodochrous | cholest-5-en-3-one + H2O2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Nocardia rhodochrous |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
13 | 44 | about 50% of maximal activity at 13°C and 44°C | Nocardia rhodochrous |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
60 h, about 25% loss of activity | Nocardia rhodochrous |
80 | - |
5 min, about 70% loss of activity | Nocardia rhodochrous |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Nocardia rhodochrous |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8 | about 50% of maximal activity at pH 4.5, about 90% of maximal activity at pH 8 | Nocardia rhodochrous |