Literature summary for 1.1.3.47 extracted from

Wu, S.; Liu, Q.; Tan, H.; Zhang, F.; Yin, H.
A novel 2,5-furandicarboxylic acid biosynthesis route from biomass-derived 5-hydroxymethylfurfural based on the consecutive enzyme reactions (2020), Appl. Biochem. Biotechnol., 191, 1470-1482 .

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Application

Application	Comment	Organism
synthesis	HMFO is used to convert 5-hydroxymethylfurfural to 2,5-diformylfuran and 5-formylfuroic acid (FFA), which is consecutively transformed to 2,5-furandicarboxylic acid by lipase Novozym 435. To facilitate the purification, a coupled alkali precipitation was developed to recover 2,5-furandicarboxylic acid from organic solvent with an improved purity from 84.4 to 99.0% and recovery of 78.1%	Methylovorus sp. MP688

Organism

Organism	UniProt	Comment	Textmining
Methylovorus sp. MP688	E4QP00	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-(hydroxymethyl)furfural + O2	-	Methylovorus sp. MP688	furan-2,5-dicarbaldehyde + H2O2	-	?
furan-2,5-dicarbaldehyde + O2	-	Methylovorus sp. MP688	5-formylfuran-2-carboxylate + H2O2	-	?
vanillyl alcohol + O2	-	Methylovorus sp. MP688	vanillin + H2O2	-	?

Subunits

Subunits	Comment	Organism
?	x * 70000, SDS-PAGE	Methylovorus sp. MP688

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Release 2023.1 (January 2023)