Cloned (Comment) | Organism |
---|---|
expression in Aspergillus oryzae under the starch inducible alpha-amylase promoter, expression in Pichia pastoris | Alternaria solani |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
prosolanapyrone II + O2 | Alternaria solani | the enzyme is involved in solanapyrone biosynthesis | prosolanapyrone III + H2O2 | - |
? | |
prosolanapyrone II + O2 | Alternaria solani 548 | the enzyme is involved in solanapyrone biosynthesis | prosolanapyrone III + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alternaria solani | D7UQ40 | - |
- |
Alternaria solani 548 | D7UQ40 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Alternaria solani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
prosolanapyrone II + O2 | the enzyme is involved in solanapyrone biosynthesis | Alternaria solani | prosolanapyrone III + H2O2 | - |
? | |
prosolanapyrone II + O2 | the bifunctional enzyme catalyses the oxidation of prosolanapyrone II and the subsequent cycloaddition of the product prosolanapyrone III to (-)-solanapyrone A | Alternaria solani | prosolanapyrone III + H2O2 | in an enzyme-catalyzed DielsAlder reaction the product prosolanapyrone III is isomerized to solanapyrone A (exo Diels-Alder cyclization, [4+2] cycloaddition). Solanapyrone D (endo Diels-Alder cyclization) is formed by a nonenzymatic Diels-Alder cyclization of prosolanopyrone III in polar solvents. The enzyme coverts prosolanapyrone II into solanapyrone A, solanapyrone D and prosolanapyrone III in the ratio 7:1:1 | ? | |
prosolanapyrone II + O2 | the enzyme is involved in solanapyrone biosynthesis | Alternaria solani 548 | prosolanapyrone III + H2O2 | - |
? | |
prosolanapyrone II + O2 | the bifunctional enzyme catalyses the oxidation of prosolanapyrone II and the subsequent cycloaddition of the product prosolanapyrone III to (-)-solanapyrone A | Alternaria solani 548 | prosolanapyrone III + H2O2 | in an enzyme-catalyzed DielsAlder reaction the product prosolanapyrone III is isomerized to solanapyrone A (exo Diels-Alder cyclization, [4+2] cycloaddition). Solanapyrone D (endo Diels-Alder cyclization) is formed by a nonenzymatic Diels-Alder cyclization of prosolanopyrone III in polar solvents. The enzyme coverts prosolanapyrone II into solanapyrone A, solanapyrone D and prosolanapyrone III in the ratio 7:1:1 | ? |
Synonyms | Comment | Organism |
---|---|---|
Sol5 | - |
Alternaria solani |
solanapyrone synthase | bifunctional enzyme: prosolanapyrone II oxidase/prosolanapyrone III cycloisomerase | Alternaria solani |
SPS | - |
Alternaria solani |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Alternaria solani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Alternaria solani |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | flavin-dependent oxidase, the enzyme possesses a flavin-binding domain | Alternaria solani |