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Literature summary for 1.1.3.41 extracted from

  • Winter, R.T.; van den Berg, T.E.; Colpa, D.I.; van Bloois, E.; Fraaije, M.W.
    Functionalization of oxidases with peroxidase activity creates oxiperoxidases: a new breed of hybrid enzyme capable of cascade chemistry (2012), ChemBioChem, 13, 252-258.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
functional expression of recombinant oxidase-peroxidase fusion mutant enzyme in Escherichia coli strain BL21(DE3) periplasm Streptomyces coelicolor

Protein Variants

Protein Variants Comment Organism
additional information construction of an AldO mutant synthetic bifunctional enzyme, the enzyme from Streptomyces coelicolor A3(2) is endowed with an extra catalytic functionality by fusing it to a microperoxidase. The mutant is functional and a both fully covalently flavinylated and heminylated: an oxiperoxidase. Replacement of portions of the wild-type AldO sequence with the bacterial cytochrome c CXXCH heme-binding motif, domain structure, overview Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29 0.35 xylitol recombinant oxidase-peroxidase fusion mutant enzyme, pH 7.5, temperature not specified in the publication Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
x * 50000, recombinant oxidase-peroxidase fusion mutant enzyme, SDS-PAGE Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylitol + O2 Streptomyces coelicolor
-
xylose + H2O2
-
?
xylitol + O2 Streptomyces coelicolor A3(2)
-
xylose + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor Q9ZBU1
-
-
Streptomyces coelicolor A3(2) Q9ZBU1
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant oxidase-peroxidase fusion mutant enzyme from Escherichia coli strain BL21(DE3) Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylitol + O2
-
Streptomyces coelicolor xylose + H2O2
-
?
xylitol + O2
-
Streptomyces coelicolor A3(2) xylose + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 50000, recombinant oxidase-peroxidase fusion mutant enzyme, SDS-PAGE Streptomyces coelicolor

Synonyms

Synonyms Comment Organism
AldO
-
Streptomyces coelicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.1 12.2 xylitol recombinant oxidase-peroxidase fusion mutant enzyme, pH 7.5, temperature not specified in the publication Streptomyces coelicolor

Cofactor

Cofactor Comment Organism Structure
FAD
-
Streptomyces coelicolor