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Literature summary for 1.1.3.4 extracted from
- Production, optimization, characterization and immobilization of glucose oxidase from Aspergillus species (2017), Res. J. Pharm. Technol., 10, 1924-1928 .
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ag2+ | 1 mM, 90-95% inhibition | Aspergillus niger |  |
| Co2+ | 1 mM, about 90% inhibition | Aspergillus niger | |
| Hg2+ | 1 mM, about 85% inhibition | Aspergillus niger | |
| Ni2+ | 1 mM, about 75% inhibition | Aspergillus niger | |
| Pb2+ | 1 mM, about 25% inhibition | Aspergillus niger | |
| Zn2+ | 1 mM, about 55% inhibition | Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 89.13 | - |
beta-D-glucose | pH and temperature not specified in the publication | Aspergillus niger | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CuCl2 | 1 mM, enhances activity by 25% | Aspergillus niger | |
| KCN | 1 mM, enhances activity by 15% | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 160000 | - |
SDS-PAGE | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Aspergillus niger PIL7 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28.81 | - |
pH and temperature not specified in the publication | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-d-glucose + O2 | 10% of the activity compared to beta-D-glucose | Aspergillus niger | ? + H2O2 | - |
? | |
| 2-deoxy-d-glucose + O2 | 10% of the activity compared to beta-D-glucose | Aspergillus niger PIL7 | ? + H2O2 | - |
? | |
| 3,6-methyl-D-glucose + O2 | 10% of the activity compared to beta-D-glucose | Aspergillus niger | ? + H2O2 | - |
? | |
| 3,6-methyl-D-glucose + O2 | 10% of the activity compared to beta-D-glucose | Aspergillus niger PIL7 | ? + H2O2 | - |
? | |
| 4-deoxy-d-glucose + O2 | 7% of the activity compared to beta-D-glucose | Aspergillus niger | ? + H2O2 | - |
? | |
| 4-O-methyl-D-glucose + O2 | 8% of the activity compared to beta-D-glucose | Aspergillus niger | ? + H2O2 | - |
? | |
| 6-deoxy-d-glucose + O2 | 12% of the activity compared to beta-D-glucose | Aspergillus niger | ? + H2O2 | - |
? | |
| beta-D-glucose + O2 | - |
Aspergillus niger | D-glucono-1,5-lactone + H2O2 | - |
? | |
| beta-D-glucose + O2 | - |
Aspergillus niger PIL7 | D-glucono-1,5-lactone + H2O2 | - |
? | |
| L-sorbose + O2 | 15% of the activity compared to beta-D-glucose | Aspergillus niger | ? + H2O2 | - |
? | |
| L-sorbose + O2 | 15% of the activity compared to beta-D-glucose | Aspergillus niger PIL7 | ? + H2O2 | - |
? | |
| mannose + O2 | 9% of the activity compared to beta-D-glucose | Aspergillus niger | ? + H2O2 | - |
? | |
| mannose + O2 | 9% of the activity compared to beta-D-glucose | Aspergillus niger PIL7 | ? + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 160000, SDS-PAGE | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GOX | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Aspergillus niger |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | 20°C: about 90% of maximal activity, 55°C: 90% of maximal activity, 60°C: 33% of maximal activity | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
half-life: 29.64h | Aspergillus niger |
| 30 | - |
half-life: 516.17 h | Aspergillus niger |
| 30 | - |
stable for a minimum of 180 min | Aspergillus niger |
| 35 | - |
half-life: 78.48 h | Aspergillus niger |
| 40 | - |
half-life: 36.42 h | Aspergillus niger |
| 45 | - |
half-life: 18.48 h | Aspergillus niger |
| 50 | - |
half-life: 11.88 h | Aspergillus niger |
| 55 | - |
half-life: 8.89 h | Aspergillus niger |
| 60 | - |
half-life: 1.38 h | Aspergillus niger |
| 65 | - |
half-life: 27.4 min | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | pH 4.5: about 60% of maximal activity, pH 7.0: about 50% of maximal activity | Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
180 min, 14% loss of activity | Aspergillus niger |
| 5.5 | - |
180 min, 13% loss of activity | Aspergillus niger |
| 6 | - |
180 min, 11% loss of activity | Aspergillus niger |
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