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Literature summary for 1.1.3.4 extracted from
- Expression, characterization and one step purification of heterologous glucose oxidase gene from Aspergillus niger ATCC 9029 in Pichia pastoris (2018), EuPA Open Proteom., 19, 1-5 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris. Cloned into the expression vector, pPIC9 and screened by the alcohol oxidase promoter. The enzyme production increases at 28°C. Enzyme activity induced by 1.0% methanol and the highest level of enzyme production is the result of shaking rate at 225 rpm | Aspergillus niger |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69000 | - |
SDS-PAGE | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | P13006 | - |
- |
| Aspergillus niger ATCC 9029 | P13006 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| one step purification | Aspergillus niger |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 69000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | 50% of enzyme activity is maintained after 90 min incubation at 20-40°C | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | recombinant enzyme | Aspergillus niger |
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