Crystallization (Comment) | Organism |
---|---|
native wild-type enzyme, hanging-drop vapor diffusion method at 4 °C by mixing equal volumes of 14 mg/mL AldO solution in 50 mM potassium phosphate buffer, pH 7.5, with reservoir solutions containing 0.1 M MES/HCl, pH 6.5, 0.2 M MgCl2 and 18-20% w/v PEG4000, 3-4 days, substrate incorporation by soaking the wild-type AldO crystals in a solution consisting of 0.1 M MES/HCl, pH 6.5, 0.2 M MgCl2, 25% w/v PEG 4000, 17.5% sucrose, and 25 mM substrate for 3 h, X-ray diffraction structure determination and analysis at 1.1-1.9 A resolution | Streptomyces coelicolor |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Streptomyces coelicolor | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + O2 | Streptomyces coelicolor | - |
? | - |
? | |
D-mannitol + O2 | Streptomyces coelicolor A3(2) | - |
? | - |
? | |
D-sorbitol + O2 | Streptomyces coelicolor | - |
? | - |
? | |
D-sorbitol + O2 | Streptomyces coelicolor A3(2) | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | - |
- |
- |
Streptomyces coelicolor A3(2) | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
beta-D-glucose + O2 = D-glucono-1,5-lactone + H2O2 | catalytic mechanism and stereoselectivity, substrate binding occurs through a lock-and-key mechanism and does not induce conformational changes with respect to the ligand-free protein, overview | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannitol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
D-mannitol + O2 | - |
Streptomyces coelicolor A3(2) | ? | - |
? | |
D-sorbitol + O2 | - |
Streptomyces coelicolor | ? | - |
? | |
D-sorbitol + O2 | - |
Streptomyces coelicolor A3(2) | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | AldO shares the same folding topology of the members of the vanillyl-alcohol oxidase family of flavoenzymes, three-dimensional structure analysis, overview | Streptomyces coelicolor |
Synonyms | Comment | Organism |
---|---|---|
AldO | - |
Streptomyces coelicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavin-dependent oxidase with covalently linked FAD which is located at the bottom of a funnel-shaped pocket that forms the active site | Streptomyces coelicolor |