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Literature summary for 1.1.3.4 extracted from

  • Van Hellemond, E.; Vermote, L.; Koolen, W.; Sonke, T.; Zandvoort, E.; Heuts, D.; Janssen, D.; Fraaije, M.
    Exploring the biocatalytic scope of alditol oxidase from Streptomyces coelicolor (2009), Adv. Synth. Catal., 351, 1523-1530.
No PubMed abstract available

Application

Application Comment Organism
synthesis AldO is an enantioselective biocatalyst for the kinetic resolution of racemic 1,2-diols Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information substrate specificity and steady state kinetics, overview Streptomyces coelicolor
0.32
-
xylitol pH 7.5, 30°C Streptomyces coelicolor
1.4
-
D-sorbitol pH 7.5, 30°C Streptomyces coelicolor
10
-
(R)-1-phenyl-1,2-ethanediol pH 7.5, 30°C Streptomyces coelicolor
25
-
L-Threitol pH 7.5, 30°C Streptomyces coelicolor
35
-
2-amino-1-pentanol pH 7.5, 30°C Streptomyces coelicolor
36
-
D-mannitol pH 7.5, 30°C Streptomyces coelicolor
42
-
4-pentene-1,2-diol pH 7.5, 30°C Streptomyces coelicolor
52
-
1,2-pentanediol pH 7.5, 30°C Streptomyces coelicolor
83
-
1-phenyl-1,2-ethanediol pH 7.5, 30°C Streptomyces coelicolor
86
-
(S)-1-phenyl-1,2-ethanediol pH 7.5, 30°C Streptomyces coelicolor
97
-
1,2-hexanediol pH 7.5, 30°C Streptomyces coelicolor
150
-
1,2-Butanediol pH 7.5, 30°C Streptomyces coelicolor
170
-
1,2,4-butanetriol pH 7.5, 30°C Streptomyces coelicolor
250
-
3-butene-1,2-diol pH 7.5, 30°C Streptomyces coelicolor
350
-
glycerol pH 7.5, 30°C Streptomyces coelicolor
430
-
L-arabinose pH 7.5, 30°C Streptomyces coelicolor
480
-
3-butenol pH 7.5, 30°C Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor
-
-
-

Reaction

Reaction Comment Organism Reaction ID
beta-D-glucose + O2 = D-glucono-1,5-lactone + H2O2 proposed mechanism for the oxidation of 1,2-diols to the alpha-hydroxy acids, overview Streptomyces coelicolor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.3
-
substrate xylitol Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-1-phenyl-1,2-ethanediol + O2
-
Streptomyces coelicolor ?
-
?
(R)-1-phenyl-1,2-ethanediol + O2
-
Streptomyces coelicolor (2R)-hydroxy(phenyl)ethanoic acid + H2O2 product identification by NMR ?
(S)-1-phenyl-1,2-ethanediol + O2
-
Streptomyces coelicolor ?
-
?
1,2,4-butanetriol + O2
-
Streptomyces coelicolor ?
-
?
1,2-butanediol + O2
-
Streptomyces coelicolor ?
-
?
1,2-hexanediol + O2
-
Streptomyces coelicolor ?
-
?
1,2-pentanediol + O2
-
Streptomyces coelicolor 2-hydroxypentanoate + H2O2 product identification by NMR ?
1,3-butanediol + O2
-
Streptomyces coelicolor 2-hydroxypropanal + H2O2 product identification by GC-MS ?
1-phenyl-1,2-ethanediol + O2
-
Streptomyces coelicolor hydroxy(phenyl)ethanoic acid + H2O2
-
r
2-amino-1-pentanol + O2
-
Streptomyces coelicolor ?
-
?
3-butene-1,2-diol + O2
-
Streptomyces coelicolor ?
-
?
3-butenol + O2
-
Streptomyces coelicolor ?
-
?
4-pentene-1,2-diol + O2
-
Streptomyces coelicolor ?
-
?
D-mannitol + O2
-
Streptomyces coelicolor ?
-
?
D-mannitol + O2
-
Streptomyces coelicolor A3(2) ?
-
?
D-sorbitol + O2
-
Streptomyces coelicolor ?
-
?
D-sorbitol + O2
-
Streptomyces coelicolor A3(2) ?
-
?
glycerol + O2
-
Streptomyces coelicolor ?
-
?
L-arabinose + O2
-
Streptomyces coelicolor ?
-
?
L-arabinose + O2
-
Streptomyces coelicolor A3(2) ?
-
?
L-threitol + O2
-
Streptomyces coelicolor ?
-
?
additional information substrate specificity,besides alditols, 1,2-diols are reasonable substrates indicating that two adjacent hydroxy groups at C-1 and C-2 seem to be a minimal requirement for a compound in order to be effectively oxidized by AldO, overview Streptomyces coelicolor ?
-
?
additional information substrate specificity,besides alditols, 1,2-diols are reasonable substrates indicating that two adjacent hydroxy groups at C-1 and C-2 seem to be a minimal requirement for a compound in order to be effectively oxidized by AldO, overview Streptomyces coelicolor A3(2) ?
-
?
xylitol + O2 best substrate Streptomyces coelicolor ?
-
?
xylitol + O2 best substrate Streptomyces coelicolor A3(2) ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Streptomyces coelicolor

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
half-life of 40 h Streptomyces coelicolor
50
-
half-life of 4 h Streptomyces coelicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.008
-
(S)-1-phenyl-1,2-ethanediol pH 7.5, 30°C Streptomyces coelicolor
0.017
-
2-amino-1-pentanol pH 7.5, 30°C Streptomyces coelicolor
0.1
-
3-butenol pH 7.5, 30°C Streptomyces coelicolor
0.29
-
1,2-Butanediol pH 7.5, 30°C Streptomyces coelicolor
0.34
-
3-butene-1,2-diol pH 7.5, 30°C Streptomyces coelicolor
0.35
-
4-pentene-1,2-diol pH 7.5, 30°C Streptomyces coelicolor
0.36
-
1-phenyl-1,2-ethanediol pH 7.5, 30°C Streptomyces coelicolor
0.74
-
(R)-1-phenyl-1,2-ethanediol pH 7.5, 30°C Streptomyces coelicolor
0.85
-
1,2-pentanediol pH 7.5, 30°C Streptomyces coelicolor
1.6
-
glycerol pH 7.5, 30°C Streptomyces coelicolor
1.7
-
L-arabinose pH 7.5, 30°C Streptomyces coelicolor
2
-
1,2-hexanediol pH 7.5, 30°C Streptomyces coelicolor
4.4
-
1,2,4-butanetriol pH 7.5, 30°C Streptomyces coelicolor
6.3
-
L-Threitol pH 7.5, 30°C Streptomyces coelicolor
9.2
-
D-mannitol pH 7.5, 30°C Streptomyces coelicolor
13
-
xylitol pH 7.5, 30°C Streptomyces coelicolor
17
-
D-sorbitol pH 7.5, 30°C Streptomyces coelicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Streptomyces coelicolor

pH Range

pH Minimum pH Maximum Comment Organism
6 9 over 80% of maximal activity within this range Streptomyces coelicolor