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Literature summary for 1.1.3.4 extracted from

  • Simpson, C.; Jordaan, J.; Gardiner, N.S.; Whiteley, C.
    Isolation, purification and characterization of a novel glucose oxidase from Penicillium sp. CBS 120262 optimally active at neutral pH (2007), Protein Expr. Purif., 51, 260-266.
    View publication on PubMed
Search for more literature for 1.1.3.4

Application

Application Comment Organism
analysis the enzyme might by useful in designing of biosensors for use in clinical, biochemical, and diagnostic assays Penicillium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
18.4
-
 beta-D-glucose pH 7.0, 25°C Penicillium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular isozyme 2 Penicillium sp.
-
-
intracellular isozyme 1 Penicillium sp. 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 2 * 70000, SDS-PAGE Penicillium sp.
148000
-
 gel filtration Penicillium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-glucose + O2 Penicillium sp.
-
 D-glucono-1,5-lactone + H2O2
-
 ?
beta-D-glucose + O2 Penicillium sp. CBS 120262
-
 D-glucono-1,5-lactone + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Penicillium sp.
-
 two isozymes
-
Penicillium sp. CBS 120262
-
 two isozymes
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 63fold by ammonium sulfate fractionation, ultrafiltration, anion exchange chromatography, and gel filtration, to 93% purity Penicillium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 1.08 U/mg dry weight for the intracellular enzyme, and 6.9U/ml for the extracellular enzyme Penicillium sp.
240.5
-
 purified enzyme Penicillium sp.

Storage Stability

Storage Stability Organism
the lyophilized purified enzyme is stable at -20 °C for a minimum of 6 months. Penicillium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucose + O2
-
 Penicillium sp. D-glucono-1,5-lactone + H2O2
-
 ?
beta-D-glucose + O2 via cofactor FAD reduction to FADH2, reaction cycles, FADH2 reduces O2, overview Penicillium sp. D-glucono-1,5-lactone + H2O2
-
 ?
beta-D-glucose + O2
-
 Penicillium sp. CBS 120262 D-glucono-1,5-lactone + H2O2
-
 ?
beta-D-glucose + O2 via cofactor FAD reduction to FADH2, reaction cycles, FADH2 reduces O2, overview Penicillium sp. CBS 120262 D-glucono-1,5-lactone + H2O2
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 70000, SDS-PAGE Penicillium sp.

Synonyms

Synonyms Comment Organism
GOX
-
 Penicillium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Penicillium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 45 70% of maximal activity at 10°C and 45°C Penicillium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 10 h, purified enzyme, stable Penicillium sp.
37
-
 half-life: 30 min, purified enzyme Penicillium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
741
-
 beta-D-glucose pH 7.0, 25°C Penicillium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Penicillium sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.9 8.9 70% of maximal activity at pH 4.9 and pH 8.9 Penicillium sp.

Cofactor

Cofactor Comment Organism Structure
FAD flavoenzyme Penicillium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Penicillium sp. intracellular isozyme 1
-
 4.3
Penicillium sp. extracellular isozyme 2
-
 4.7