Application | Comment | Organism |
---|---|---|
analysis | the enzyme finds wide application in food industry and clinical analysis | Talaromyces variabilis |
Cloned (Comment) | Organism |
---|---|
gene GOX, DNA sequence determination and analysis, expression in a larger scale in Pichia pastoris strain X33 using the methanol inducible AOX1 promoter, the enzyme is secreted | Talaromyces variabilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.5 | - |
D-glucose | pH 5.0, 28°C, native enzyme | Talaromyces variabilis | |
15.25 | - |
D-glucose | pH 5.0, 28°C, recombinant enzyme | Talaromyces variabilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
2 * 82000, recombinant enzyme, SDS-PAGE | Talaromyces variabilis |
175000 | - |
gel filtration | Talaromyces variabilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + O2 | Talaromyces variabilis | - |
D-glucono-1,5-lactone + H2O2 | - |
? | |
D-glucose + O2 | Talaromyces variabilis P16 | - |
D-glucono-1,5-lactone + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Talaromyces variabilis | Q70FC9 | - |
- |
Talaromyces variabilis P16 | Q70FC9 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the recombinant enzyme from Pichia pastoris is higher glycosylated, 17%, compared to the native enzyme, 14% | Talaromyces variabilis |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 2.4fold from Pichia pastoris culture supernatant by ultrafiltration, anion exchange chromatography, and gel filtration | Talaromyces variabilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.33 | - |
secreted enzyme in crude cell culture supernatant | Talaromyces variabilis |
144 | - |
purified recombinant enzyme | Talaromyces variabilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-deoxy-D-glucose + O2 | 19.6% of the activity with D-glucose for the native enzyme, 5.9 for the recombinant enzyme | Talaromyces variabilis | ? | - |
? | |
D-fructose + O2 | 4.9% of the activity with D-glucose for the native enzyme, no activity with the recombinant enzyme | Talaromyces variabilis | ? | - |
? | |
D-glucose + O2 | - |
Talaromyces variabilis | D-glucono-1,5-lactone + H2O2 | - |
? | |
D-glucose + O2 | best substrate | Talaromyces variabilis | D-glucono-1,5-lactone + H2O2 | - |
? | |
D-glucose + O2 | - |
Talaromyces variabilis P16 | D-glucono-1,5-lactone + H2O2 | - |
? | |
D-glucose + O2 | best substrate | Talaromyces variabilis P16 | D-glucono-1,5-lactone + H2O2 | - |
? | |
D-mannose + O2 | 7.2% of the activity with D-glucose for the native enzyme, 13.4 for the recombinant enzyme | Talaromyces variabilis | ? | - |
? | |
D-mannose + O2 | 7.2% of the activity with D-glucose for the native enzyme, 13.4 for the recombinant enzyme | Talaromyces variabilis P16 | ? | - |
? | |
D-xylose + O2 | 3.0% of the activity with D-glucose for the native enzyme, 5.8 for the recombinant enzyme | Talaromyces variabilis | ? | - |
? | |
D-xylose + O2 | 3.0% of the activity with D-glucose for the native enzyme, 5.8 for the recombinant enzyme | Talaromyces variabilis P16 | ? | - |
? | |
maltose + O2 | 21.3% of the activity with D-glucose for the native enzyme, 42.2% for the recombinant enzyme | Talaromyces variabilis | ? | - |
? | |
additional information | no activity with L-arabinose and D-galactose with the native and recombinant enzyme | Talaromyces variabilis | ? | - |
? | |
additional information | no activity with L-arabinose and D-galactose with the native and recombinant enzyme | Talaromyces variabilis P16 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 82000, recombinant enzyme, SDS-PAGE | Talaromyces variabilis |
Synonyms | Comment | Organism |
---|---|---|
GOX | - |
Talaromyces variabilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant enzyme | Talaromyces variabilis |
55 | - |
native enzyme | Talaromyces variabilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | temperature profile of native enzyme, overview | Talaromyces variabilis |
30 | 55 | temperature profile of recombinant enzyme, overview | Talaromyces variabilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
half-life native enzyme: 103 min, half-life recombinant enzyme: 26 min | Talaromyces variabilis |
45 | - |
half-life native enzyme: 23 min, half-life recombinant enzyme: 3 min | Talaromyces variabilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | native enzyme and recombinant enzyme at pH 5.0, 28°C | Talaromyces variabilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
native and recombinant enzyme | Talaromyces variabilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | pH-profiles, 54% of maximal activity at pH 3.0, 20% of maximal activity at pH 8.0 for the recombinant enzyme, 50% for the native enzyme, overview | Talaromyces variabilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
half-life native enzyme: 166.3 min | Talaromyces variabilis |
7 | - |
half-life native enzyme: 76.3 min | Talaromyces variabilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | bound | Talaromyces variabilis |