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Literature summary for 1.1.3.4 extracted from
- Purification and characterization of glucose oxidase from ligninolytic cultures of Phanerochaete chrysosporium (1986), J. Bacteriol., 166, 269-274.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| CuCl2 | stimulation of activity, 123% relative activity to no addition | Phanerodontia chrysosporium |  |
| KCN | stimulation of activity, 135% relative activity to no addition | Phanerodontia chrysosporium | |
| NaF | weak stimulation of activity, 111% relative activity to no addition | Phanerodontia chrysosporium | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 75% inhibition at 10 mM | Phanerodontia chrysosporium | |
| CN- | not inhibitory | Phanerodontia chrysosporium | |
| CuSO4 | not inhibitory | Phanerodontia chrysosporium | |
| F- | not inhibitory | Phanerodontia chrysosporium | |
| o-phthalate | 88% inhibition at 50 mM | Phanerodontia chrysosporium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.95 | - |
O2 | - |
Phanerodontia chrysosporium | |
| 37 | 38 | beta-D-glucose | - |
Phanerodontia chrysosporium | |
| 38 | - |
beta-D-glucose | - |
Phanerodontia chrysosporium | |
| 55.5 | - |
D-maltose | - |
Phanerodontia chrysosporium | |
| 105.2 | - |
D-xylose | - |
Phanerodontia chrysosporium | |
| 217.4 | - |
L-sorbose | - |
Phanerodontia chrysosporium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 175000 | 180000 | gel filtration | Phanerodontia chrysosporium |
| 180000 | - |
gel filtration, overestimation may be due to hydrodynamic properties of the enzyme | Phanerodontia chrysosporium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-glucose + O2 + H2O | Phanerodontia chrysosporium | - |
D-glucono-1,5-lactone + H2O2 | - |
? | |
| additional information | Phanerodontia chrysosporium | the enzyme is the predominant source of H2O2 in ligninolytic cultures, H2O2 plays a central role in lignin biodegradation, it is obligately required for the activity of ligninases, a family of lignin peroxidases that is important in the oxidative depolymerization of lignin | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | - |
Phanerodontia chrysosporium |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using column chromatography on DEAE-Sephadex, Sephacryl S-300 and DEAE-Sepharose | Phanerodontia chrysosporium |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Phanerodontia chrysosporium | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.1 | - |
oxidation of o-dianisidine at 37°C and pH 4.5 | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-D-glucose + O2 + H2O | - |
Phanerodontia chrysosporium | D-glucono-1,5-lactone + H2O2 | - |
? | |
| beta-D-glucose + O2 + H2O | glucose is the primary substrate for the enzyme | Phanerodontia chrysosporium | D-glucono-1,5-lactone + H2O2 | - |
? | |
| D-maltose + O2 + H2O | 4.5% of D-glucose reactivity | Phanerodontia chrysosporium | ? | - |
? | |
| D-xylose + O2 + H2O | 4.8% of D-glucose reactivity | Phanerodontia chrysosporium | ? | - |
? | |
| L-sorbose + O2 + H2O | 5.8% of D-glucose reactivity | Phanerodontia chrysosporium | ? | - |
? | |
| additional information | the enzyme is the predominant source of H2O2 in ligninolytic cultures, H2O2 plays a central role in lignin biodegradation, it is obligately required for the activity of ligninases, a family of lignin peroxidases that is important in the oxidative depolymerization of lignin | Phanerodontia chrysosporium | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | 5 | - |
Phanerodontia chrysosporium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 1.5 mol of flavin per mol of protein | Phanerodontia chrysosporium | |
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