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Literature summary for 1.1.3.38 extracted from

  • De Jong, E.; van Berkel, W.J.H.; van der Zwan, R.P.; de Bont, J.A.M.
    Purification and characterization of vanillyl-alcohol oxidase from Penicillium simplicissimum. A novel aromatic alcohol oxidase containing covalently bound FAD (1992), Eur. J. Biochem., 208, 651-657.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxyphenylethyl alcohol
-
Penicillium simplicissimum
cinnamyl alcohol strong competitive inhibitor of vanillyl-alcohol oxidation Penicillium simplicissimum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
O2
-
Penicillium simplicissimum
0.043
-
phenazine methosulfate
-
Penicillium simplicissimum
0.73
-
Vanillyl alcohol
-
Penicillium simplicissimum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
8 * 65000, SDS-PAGE Penicillium simplicissimum
520000
-
gel filtration Penicillium simplicissimum

Organism

Organism UniProt Comment Textmining
Penicillium simplicissimum
-
-
-

Oxidation Stability

Oxidation Stability Organism
photoreduction of the enzyme in the presence of the competitive inhibitor cinnamyl alcohol gives rise to a complete irreversible bleaching of the flavin spectrum Penicillium simplicissimum

Purification (Commentary)

Purification (Comment) Organism
-
Penicillium simplicissimum

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:veratryl alcohol-grown cell sole source of carbon and energy Penicillium simplicissimum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.13
-
-
Penicillium simplicissimum

Storage Stability

Storage Stability Organism
-20°C or 4°C, in 20% (NH4)2SO4, pH 7.0, stable for several weeks Penicillium simplicissimum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxybenzyl alcohol + O2 at 75% of the activity with vanillyl alcohol Penicillium simplicissimum 4-hydroxybenzaldehyde + H2O2
-
?
vanillyl alcohol + O2
-
Penicillium simplicissimum vanillin + H2O2
-
?
vanillyl alcohol + phenazine methosulfate
-
Penicillium simplicissimum vanillin + reduced phenazine methosulfate
-
?

Subunits

Subunits Comment Organism
octamer 8 * 65000, SDS-PAGE Penicillium simplicissimum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
-
Penicillium simplicissimum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
90 min, stable Penicillium simplicissimum
60
-
45 min, 50% loss of activity Penicillium simplicissimum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 10.5
-
Penicillium simplicissimum

pH Range

pH Minimum pH Maximum Comment Organism
7 12 about 30% of maximal activity at pH 7 and pH 12 Penicillium simplicissimum

Cofactor

Cofactor Comment Organism Structure
8alpha-(N3-Histidyl)-FAD the octamer contains 1 mol of covalently-bound flavin per mol of subunit Penicillium simplicissimum