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Literature summary for 1.1.3.20 extracted from

  • Banthorpe, D.V.; Cardemil, E.; Del Carmen Contraras, M.
    Purification and properties of alcohole oxidase from Tanacetum vulgare (1976), Phytochemistry, 15, 391-394.
No PubMed abstract available
Search for more literature for 1.1.3.20

General Stability

General Stability Organism
prolonged dialysis against EDTA, stable to Tanacetum vulgare

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibitor: cyanide; no inhibitors: iodoacetate, MnSO4, EDTA, p-hydroxymercuribenzoate, 2,2'-dipyridyl, 1,10-phenanthroline, 8-hydroxyquinoline, fluoride, azide Tanacetum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
 Hex-trans-2-ene-1-ol
-
 Tanacetum vulgare
0.49
-
 n-Octanol value above Tanacetum vulgare
1.56
-
 geraniol
-
 Tanacetum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 1 * 94000 + 1 * 75000, SDS-PAGE Tanacetum vulgare
94000
-
 1 * 94000 + 1 * 75000, SDS-PAGE Tanacetum vulgare
180000
-
 gel filtration Tanacetum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
long-chain alcohol + O2 Tanacetum vulgare substrates are primary alcohols with a chain-length of C6-C10 ?
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone stable to Tanacetum vulgare

Organism

Organism UniProt Comment Textmining
Tanacetum vulgare
-
-
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Tanacetum vulgare

Reaction

Reaction Comment Organism Reaction ID
a long-chain alcohol + O2 = a long-chain aldehyde + H2O2 2 long-chain alcohol + O2 = 2 long-chain aldehyde + 2 H2O2 Tanacetum vulgare
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Tanacetum vulgare
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6
-
-
 Tanacetum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geraniol + O2 i.e. 3,7-dimethylocta-trans-2,6-dien-1-ol, better substrate than cis-isomer, the 2-ene-group enhances activity Tanacetum vulgare 3,7-dimethylocta-trans-2,6-dien-1-al + H2O2
-
 ?
hex-trans-2-ene-1-ol + O2 preferred substrate, the 2-ene-group enhances activity Tanacetum vulgare hex-trans-2-ene-1-al + H2O2
-
 ?
long-chain alcohol + O2 substrates are primary alcohols with a chain-length of C6-C10 Tanacetum vulgare ?
-
 ?
additional information substrates are primary alcohols with a chain-length of C6-C10, optimal chain-length: C8-C10 Tanacetum vulgare ?
-
 ?
additional information no substrates for Tanacetum enzyme: secondary, tertiary alcohols and Tris Tanacetum vulgare ?
-
 ?
n-octanol + O2
-
 Tanacetum vulgare n-octanal + H2O2
-
 ?
terpenol + O2 poor substrate Tanacetum vulgare ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 94000 + 1 * 75000, SDS-PAGE Tanacetum vulgare

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
18 22
-
 Tanacetum vulgare

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 45 about 60% of maximal activity at 0°C and about 30% of maximal activity at 45°C Tanacetum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 9 plateau Tanacetum vulgare

pH Range

pH Minimum pH Maximum Comment Organism
4.8 10 about half-maximal activity at pH 4.8 and 10.0 Tanacetum vulgare

Cofactor

Cofactor Comment Organism Structure
additional information NAD(P)+ Tanacetum vulgare
additional information FAD, FMN Tanacetum vulgare