Cloned (Comment) | Organism |
---|---|
overexpression of the enzyme, fused to a chloroplast-transit peptide from Pisum sativum, in transgenic Oryza sativa plants using the Agrobacterium tumefaciens transfection system | Arthrobacter pascens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | evaluation of the stress-inducible production of Arthrobacter pascens choline oxidase in transgenic rice as a strategy for producing the stress-protectant glycine betaine, stress induction by 150 mM NaCl, biomass production in wild-type and transgenic plants | Arthrobacter pascens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + O2 | Arthrobacter pascens | - |
betaine aldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter pascens | - |
gene COX | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme partially from stressed and non-stressed transgenic rice plant leaves by ammonium sulfate fractionation and desalting gel filtration | Arthrobacter pascens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
choline + O2 = betaine aldehyde + H2O2 | the enzyme catalyzes the oxidation of choline to glycine betaine via the intermediate betaine aldehyde | Arthrobacter pascens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity measurement in a coupled assay with NAD+-dependent betaine aldehyde dehydrogenase, BADH | Arthrobacter pascens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
choline + O2 | - |
Arthrobacter pascens | betaine aldehyde + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
COX | - |
Arthrobacter pascens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Arthrobacter pascens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Arthrobacter pascens |