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Literature summary for 1.1.3.15 extracted from

  • Maeda-Yorita, K.; Aki, K.; Sagai, H.; Misaki, H.; Massey, V.
    L-Lactate oxidase and L-lactate monooxygenase: mechanistic variations on a common structural theme (1995), Biochimie, 77, 631-642.
    View publication on PubMed
Search for more literature for 1.1.3.15

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aerococcus viridans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 O2 4°C, pH 7 Aerococcus viridans
0.16
-
 O2 25°C, pH 7 Aerococcus viridans
0.34
-
 L-lactate 4°C, pH 7 Aerococcus viridans
0.94
-
 L-lactate 25°C, pH 7 Aerococcus viridans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 gel filtration Aerococcus viridans

Organism

Organism UniProt Comment Textmining
Aerococcus viridans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aerococcus viridans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
210
-
-
 Aerococcus viridans

Storage Stability

Storage Stability Organism
-20°C, lyophilized powder containing 70-80% sucrose, stable for more than a year Aerococcus viridans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-lactate + O2
-
 Aerococcus viridans pyruvate + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
L-lactate monooxygenase
-
 Aerococcus viridans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.52
-
 O2 4°C, pH 7 Aerococcus viridans
0.52
-
 L-lactate 4°C, pH 7 Aerococcus viridans
4.7
-
 O2 25°C, pH 7 Aerococcus viridans
4.7
-
 L-lactate 25°C, pH 7 Aerococcus viridans

Cofactor

Cofactor Comment Organism Structure
FMN flavoprotein Aerococcus viridans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1400
-
 O2 4°C, pH 7 Aerococcus viridans
1800
-
 O2 25°C, pH 7 Aerococcus viridans