Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.3.15 extracted from

  • Yorita, K.; Janko, K.; Aki, K.; Ghisla, S.; Palfey, B.A.; Massey, V.
    On the reaction mechanism of L-lactate oxidase: quantitative structure-activity analysis of the reaction with para-substituted L-mandelates (1997), Proc. Natl. Acad. Sci. USA, 94, 9590-9595.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.3.15

Protein Variants

Protein Variants Comment Organism
A95G enhanced activity with bulky substrates, increase in the reduction rate constants with substrates having a bulky alpha-substituent such as mandelate, when compared with wild-type enzyme Aerococcus viridans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information reduction rate constants and dissociation constants for reaction of lactate oxidases with alpha-1H- and alpha-2H-para-substituted mandelates Aerococcus viridans

Organism

Organism UniProt Comment Textmining
Aerococcus viridans
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-mandelate + O2 the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive Aerococcus viridans phenylglyoxylic acid + H2O2
-
 ?
DL-p-hydroxymandelate + O2 the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive Aerococcus viridans ?
-
 ?
L-4-chloromandelate + O2 the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive Aerococcus viridans ?
-
 ?
L-4-fluoromandelate + O2 the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive Aerococcus viridans ?
-
 ?
L-4-methoxymandelate + O2 the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive Aerococcus viridans ?
-
 ?
L-4-methylmandelate + O2 the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive Aerococcus viridans ?
-
 ?
L-4-nitromandelate + O2 the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive Aerococcus viridans ?
-
 ?
L-4-trifluoromethylmandelate + O2 the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive Aerococcus viridans ?
-
 ?
L-mandelate + O2 the step involving the removal of the alpha-hydrogen is rate-limiting, A95G-mutant is also reactive Aerococcus viridans ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Aerococcus viridans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information reduction rate constants and dissociation constants for reaction of lactate oxidases with alpha-1H- and alpha-2H-para-substituted mandelates Aerococcus viridans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Aerococcus viridans