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Literature summary for 1.1.3.15 extracted from

  • Nakano, M.; Danowski, T.S.
    Crystalline mammalian L-amino acid oxidase from rat kidney mitochondria (1966), J. Biol. Chem., 241, 2075-2083.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.68
-
L-lactate
-
Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Rattus sp. 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88900
-
sedimentation equilibrium analysis Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DL-2-hydroxybutyrate + O2 Rattus sp.
-
2-oxobutyrate + H2O2
-
?
DL-2-hydroxycaproate + O2 Rattus sp.
-
2-oxocaproate + H2O2
-
?
DL-2-hydroxyisocaproate + O2 Rattus sp.
-
2-oxoisocaproate + H2O2
-
?
DL-2-hydroxyisovalerate + O2 Rattus sp.
-
2-oxoisovalerate + H2O2
-
?
DL-2-hydroxyvalerate + O2 Rattus sp.
-
2-oxovalerate + H2O2
-
?
L-2-hydroxyisocaproate + O2 Rattus sp.
-
2-oxoisocaproate + H2O2
-
?
L-alanine + O2 Rattus sp. low activity ? + H2O2
-
?
L-isoleucine + O2 Rattus sp. low activity ? + H2O2
-
?
L-lactate + O2 Rattus sp.
-
pyruvate + H2O2
-
?
L-leucine + O2 Rattus sp. highest activity ? + H2O2
-
?
L-methionine + O2 Rattus sp.
-
? + H2O2
-
?
L-phenylalanine + O2 Rattus sp.
-
? + H2O2
-
?
L-tryptophan + O2 Rattus sp.
-
? + H2O2
-
?
L-tyrosine + O2 Rattus sp.
-
? + H2O2
-
?
L-valine + O2 Rattus sp. low activity ? + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to crystalline state, chromatography and precipitation techniques Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Rattus sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-2-hydroxybutyrate + O2
-
Rattus sp. 2-oxobutyrate + H2O2
-
?
DL-2-hydroxycaproate + O2
-
Rattus sp. 2-oxocaproate + H2O2
-
?
DL-2-hydroxyisocaproate + O2
-
Rattus sp. 2-oxoisocaproate + H2O2
-
?
DL-2-hydroxyisovalerate + O2
-
Rattus sp. 2-oxoisovalerate + H2O2
-
?
DL-2-hydroxyvalerate + O2
-
Rattus sp. 2-oxovalerate + H2O2
-
?
DL-3-indolelactate + O2
-
Rattus sp. 3-indolepyruvate + H2O2
-
?
DL-3-methoxy-4-hydroxymandelate + 2,6-dichlorophenolindophenol
-
Rattus sp. (3-methoxy-4-hydroxyphenyl)pyruvate + reduced 2,6-dichlorophenolindophenol
-
?
DL-phenyllactate + O2
-
Rattus sp. phenylpyruvate + H2O2
-
?
L-2-hydroxyisocaproate + O2
-
Rattus sp. 2-oxoisocaproate + H2O2
-
?
L-2-hydroxyphenyllactate + O2
-
Rattus sp. ? + H2O2
-
?
L-alanine + O2 low activity Rattus sp. ? + H2O2
-
?
L-isoleucine + O2 low activity Rattus sp. ? + H2O2
-
?
L-lactate + O2
-
Rattus sp. pyruvate + H2O2
-
?
L-leucine + O2 highest activity Rattus sp. ? + H2O2
-
?
L-mandelate + 2,6-dichlorophenolindophenol
-
Rattus sp. oxo(phenyl)acetic acid + reduced 2,6-dichlorophenolindophenol
-
?
L-methionine + O2
-
Rattus sp. ? + H2O2
-
?
L-phenylalanine + O2
-
Rattus sp. ? + H2O2
-
?
L-tryptophan + O2
-
Rattus sp. ? + H2O2
-
?
L-tyrosine + O2
-
Rattus sp. ? + H2O2
-
?
L-valine + O2 low activity Rattus sp. ? + H2O2
-
?

Synonyms

Synonyms Comment Organism
L-amino acid oxidase
-
Rattus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.433
-
L-lactate
-
Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
oxidation of L-lactate Rattus sp.
8.7
-
oxidation of L-leucine Rattus sp.

Cofactor

Cofactor Comment Organism Structure
FMN 2 mol FAD per mol enzyme Rattus sp.