Protein Variants | Comment | Organism |
---|---|---|
additional information | the alcohol oxidase is immobilized on nanoporous aluminium oxide membranes by silanization and activation by carbonyldiimidazole to create a flow-through enzyme reactor, method optimization, overview. The immobilized alcohol oxidase shows high productivity in hydrogen peroxide generation from methanol, and retains about 58% of initial activity after 3 weeks of storage and repeated use with pH 6.5 and at room temperature. Construction of a bienzymatic modular reactor with horseradish peroxidase and alcohol oxidase, overview | Komagataella pastoris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, overview | Komagataella pastoris | |
0.47 | - |
methanol | pH 6.5, 22°C, immobilized enzyme | Komagataella pastoris | |
0.75 | - |
ethanol | pH 6.5, 22°C, immobilized enzyme | Komagataella pastoris | |
1.23 | - |
n-Propanol | pH 6.5, 22°C, immobilized enzyme | Komagataella pastoris | |
1.59 | - |
formaldehyde | pH 6.5, 22°C, immobilized enzyme | Komagataella pastoris | |
2.79 | - |
n-butanol | pH 6.5, 22°C, immobilized enzyme | Komagataella pastoris | |
8 | 9 | ethylene glycol | pH 6.5, 22°C, immobilized enzyme | Komagataella pastoris | |
261 | - |
2-propanol | pH 6.5, 22°C, immobilized enzyme | Komagataella pastoris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
4 * 80000, SDS-PAGE | Komagataella pastoris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + O2 | Komagataella pastoris | - |
formaldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Komagataella pastoris | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-propanol + O2 | - |
Komagataella pastoris | 2-oxopropane + H2O2 | - |
? | |
ethanol + O2 | - |
Komagataella pastoris | acetaldehyde + H2O2 | - |
? | |
ethylene glycol + O2 | - |
Komagataella pastoris | ? + H2O2 | - |
? | |
formaldehyde + O2 | - |
Komagataella pastoris | formic acid + H2O2 | - |
? | |
methanol + O2 | - |
Komagataella pastoris | formaldehyde + H2O2 | - |
? | |
additional information | substrate specificity, overview. No activity with isobutanol, benzyl alcohol, and cyclohexanol | Komagataella pastoris | ? | - |
? | |
n-butanol + O2 | - |
Komagataella pastoris | butanal + H2O2 | - |
? | |
n-propanol + O2 | - |
Komagataella pastoris | propanal + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 80000, SDS-PAGE | Komagataella pastoris |
Synonyms | Comment | Organism |
---|---|---|
AOX | - |
Komagataella pastoris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Komagataella pastoris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
assay at | Komagataella pastoris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Komagataella pastoris |