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Literature summary for 1.1.3.13 extracted from

  • Gvozdev, A.R.; Tukhvatullin, I.A.; Gvozdev, R.I.
    Purification and properties of alcohol oxidase from Pichia putida (2010), Biochemistry (Moscow), 75, 242-248.
    View publication on PubMed

General Stability

General Stability Organism
the enzyme has increased stability in phosphate-citrate buffer and low stability in Tris-HCl and phosphate buffers. Its stability is increased 10fold in the presence of sucrose, mannitol, polyethylene glycol, polyvinyl alcohol, polyvinylpyrrolidone, and EDTA. Sucrose at 50% concentration (w/v) enhances stability of the enzyme 10fold at 50°C Pichia putida

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate) complete inhibition at molar excess of reagents in relation to alcohol oxidase 20:1 Pichia putida
p-chloromercuribenzoate complete inhibition at molar excess of reagents in relation to alcohol oxidase 20:1 Pichia putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
74000
-
8 * 74000, SDS-PAGE Pichia putida
580000
-
analytical ultracentrifugation Pichia putida
590000
-
gel filtration Pichia putida

Organism

Organism UniProt Comment Textmining
Pichia putida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-cellulose column chromatography, Sepharose 2B column chromatography, and Sephadex G-25 gel filtration Pichia putida

Storage Stability

Storage Stability Organism
-20 to -80°C, 0.02 M phosphate-citrate buffer, pH 8-9, in presence of 30-50% (w/v) sucrose, 4 years, no loss of activity Pichia putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + O2 92% activity compared to methanol Pichia putida acetaldehyde + H2O2
-
?
formaldehyde + O2 35% activity compared to methanol Pichia putida formate + H2O2
-
?
methanol + O2 100% activity Pichia putida formaldehyde + H2O2
-
?
n-butanol + O2 40% activity compared to methanol Pichia putida n-butanal + H2O2
-
?
n-pentanol + O2 3% activity compared to methanol Pichia putida n-pentanal + H2O2
-
?
n-propanol + O2 75% activity compared to methanol Pichia putida n-propanal + H2O2
-
?

Subunits

Subunits Comment Organism
homooctamer 8 * 74000, SDS-PAGE Pichia putida

Synonyms

Synonyms Comment Organism
alcohol:dioxygen-oxidoreductase
-
Pichia putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 60 10% loss of activity at 20°C after 24 h, complete loss of activity after 10 min at 60°C Pichia putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9
-
Pichia putida

Cofactor

Cofactor Comment Organism Structure
FAD each subunit includes one molecule of FAD Pichia putida