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Literature summary for 1.1.3.13 extracted from
- Immobilization of Hansenula polymorpha alcohol oxidase for alcohol biosensor applications (2009), Bull. Korean Chem. Soc., 30, 57-60.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | the enzyme is useful in alcohol biosensor applications | Ogataea angusta |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | enzyme immobilization on DEAE-cellulose particles for alcohol biosensor applications, substrate specificity and the optimum pH of the immobilized enzyme are similar to those of the free enzyme, while Km and temperature optimum differ, overview | Ogataea angusta |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.66 | - |
ethanol | free enzyme | Ogataea angusta |  |
| 14.45 | - |
ethanol | immobilized enzyme | Ogataea angusta | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethanol + O2 | Ogataea angusta | - |
acetaldehyde + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ogataea angusta | - |
ATCC 26012 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme to homogeneity by two different steps of anion exchange chromatography | Ogataea angusta |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.21 | - |
free native enzyme, substrate n-propanol | Ogataea angusta |
| 0.33 | - |
immobilized native enzyme, substrate n-propanol | Ogataea angusta |
| 0.48 | - |
immobilized native enzyme, substrate crotyl alcohol | Ogataea angusta |
| 0.68 | - |
free native enzyme, substrate crotyl alcohol | Ogataea angusta |
| 6.68 | - |
immobilized native enzyme, substrate methanol | Ogataea angusta |
| 8.18 | - |
immobilized native enzyme, substrate ethanol | Ogataea angusta |
| 9.48 | - |
free native enzyme, substrate methanol | Ogataea angusta |
| 10.23 | - |
free native enzyme, substrate ethanol | Ogataea angusta |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 25°C, purified free enzyme, 10 weeks, 20% remaining actiivty | Ogataea angusta |
| 25°C, purified immobilized enzyme, 10 weeks, 60% remaining actiivty | Ogataea angusta |
| 4°C, purified free enzyme, 10 weeks, 70% remaining actiivty | Ogataea angusta |
| 4°C, purified immobilized enzyme, 10 weeks, 90% remaining actiivty | Ogataea angusta |
| the immobilized enzyme shows a high stability against long storage | Ogataea angusta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| crotyl alcohol + O2 | - |
Ogataea angusta | (2E)-but-2-enal + H2O2 | - |
? | |
| ethanol + O2 | - |
Ogataea angusta | acetaldehyde + H2O2 | - |
? | |
| methanol + O2 | - |
Ogataea angusta | formaldehyde + H2O2 | - |
? | |
| additional information | no or poor activity with 1-butanol, 2-butanol, 3-butanol, isoamyl alcohol, 2-propanol, and 2,2,2-trichloroethanol | Ogataea angusta | ? | - |
? | |
| n-propanol + O2 | - |
Ogataea angusta | propanaldehyde + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AOd | - |
Ogataea angusta |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
free enzyme | Ogataea angusta |
| 65 | - |
immobilized enzyme | Ogataea angusta |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | activity range of the immobilized enzyme, 25% of maximal activity at 80°C | Ogataea angusta |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 80 | the immobilized enzyme is fairly stable at temperature up to 60°C. Above 70°C, its activity declines rapidly as the temperature increased, but the immobilized enzyme is not completely inactivated even at 80°C | Ogataea angusta |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Ogataea angusta |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | the free and immobilized enzyme show less than 45% of their maximum activities below pH 4.0 and approximately 85% at pH 10.0 | Ogataea angusta |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Ogataea angusta | methanol induces at 0.5% | up |
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