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Literature summary for 1.1.3.13 extracted from

  • Danneel, H.J.; Reichert, A.; Giffhorn, F.
    Production, purification and characterization of an alcohol oxidase of the ligninolytic fungus Peniophora gigantea (1994), J. Biotechnol., 33, 33-41.
No PubMed abstract available

General Stability

General Stability Organism
rapid inactivation by repeated freezing and thawing Phlebiopsis gigantea

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM CuCl2, complete inhibition Phlebiopsis gigantea
FeSO4 1 mM, complete inhibition Phlebiopsis gigantea
NiCl2 1 mM, complete inhibition Phlebiopsis gigantea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
O2
-
Phlebiopsis gigantea
1.8
-
methanol
-
Phlebiopsis gigantea
2.9
-
ethanol
-
Phlebiopsis gigantea
71.5
-
D-ribose
-
Phlebiopsis gigantea
250
-
ribitol
-
Phlebiopsis gigantea
670
-
erythritol
-
Phlebiopsis gigantea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72500
-
8 * 72500, SDS-PAGE Phlebiopsis gigantea
576000
-
gel filtration Phlebiopsis gigantea

Organism

Organism UniProt Comment Textmining
Phlebiopsis gigantea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Phlebiopsis gigantea

Source Tissue

Source Tissue Comment Organism Textmining
mycelium produced in the stationary growth phase Phlebiopsis gigantea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.7
-
-
Phlebiopsis gigantea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
allylalcohol + O2
-
Phlebiopsis gigantea acrolein + H2O2
-
?
D-ribose + O2
-
Phlebiopsis gigantea ? + H2O2
-
?
erythritol + O2
-
Phlebiopsis gigantea ? + H2O2
-
?
ethanol + O2
-
Phlebiopsis gigantea acetaldehyde + H2O2
-
?
isopropanol + O2
-
Phlebiopsis gigantea 2-propanone + H2O2
-
?
methanol + O2
-
Phlebiopsis gigantea formaldehyde + H2O2
-
?
n-butanol + O2
-
Phlebiopsis gigantea n-butanal + H2O2
-
?
n-propanol + O2
-
Phlebiopsis gigantea n-propanal + H2O2
-
?
ribitol + O2
-
Phlebiopsis gigantea ? + H2O2
-
?

Subunits

Subunits Comment Organism
octamer 8 * 72500, SDS-PAGE Phlebiopsis gigantea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
50% loss of activity after 10 min in 0.1 M potassium phosphate buffer, pH 6.0. 50% loss of activity after 60 min in 0.1 M potassium phosphate buffer, pH 7.0, 50% loss of activity after 28 h in 0.1 M Tris-HCl buffer, pH 8.0. 50% loss of activity after 16 h in 0.1 M Tris-HCl buffer, pH 9.0 Phlebiopsis gigantea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3 9
-
Phlebiopsis gigantea

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
22°C, 50% loss of activity after 10 min Phlebiopsis gigantea
7
-
22°C, 50% loss of activity after 60 min Phlebiopsis gigantea
8
-
22°C, 50% loss of activity after 28 h Phlebiopsis gigantea
9
-
22°C, 50% loss of activity after 10 min Phlebiopsis gigantea

Cofactor

Cofactor Comment Organism Structure
FAD covalently bound Phlebiopsis gigantea
FAD 1 mol FAD per mol of subunit Phlebiopsis gigantea