General Stability | Organism |
---|---|
rapid inactivation by repeated freezing and thawing | Phlebiopsis gigantea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 1 mM CuCl2, complete inhibition | Phlebiopsis gigantea | |
FeSO4 | 1 mM, complete inhibition | Phlebiopsis gigantea | |
NiCl2 | 1 mM, complete inhibition | Phlebiopsis gigantea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
O2 | - |
Phlebiopsis gigantea | |
1.8 | - |
methanol | - |
Phlebiopsis gigantea | |
2.9 | - |
ethanol | - |
Phlebiopsis gigantea | |
71.5 | - |
D-ribose | - |
Phlebiopsis gigantea | |
250 | - |
ribitol | - |
Phlebiopsis gigantea | |
670 | - |
erythritol | - |
Phlebiopsis gigantea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72500 | - |
8 * 72500, SDS-PAGE | Phlebiopsis gigantea |
576000 | - |
gel filtration | Phlebiopsis gigantea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phlebiopsis gigantea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Phlebiopsis gigantea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | produced in the stationary growth phase | Phlebiopsis gigantea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.7 | - |
- |
Phlebiopsis gigantea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
allylalcohol + O2 | - |
Phlebiopsis gigantea | acrolein + H2O2 | - |
? | |
D-ribose + O2 | - |
Phlebiopsis gigantea | ? + H2O2 | - |
? | |
erythritol + O2 | - |
Phlebiopsis gigantea | ? + H2O2 | - |
? | |
ethanol + O2 | - |
Phlebiopsis gigantea | acetaldehyde + H2O2 | - |
? | |
isopropanol + O2 | - |
Phlebiopsis gigantea | 2-propanone + H2O2 | - |
? | |
methanol + O2 | - |
Phlebiopsis gigantea | formaldehyde + H2O2 | - |
? | |
n-butanol + O2 | - |
Phlebiopsis gigantea | n-butanal + H2O2 | - |
? | |
n-propanol + O2 | - |
Phlebiopsis gigantea | n-propanal + H2O2 | - |
? | |
ribitol + O2 | - |
Phlebiopsis gigantea | ? + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 72500, SDS-PAGE | Phlebiopsis gigantea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
50% loss of activity after 10 min in 0.1 M potassium phosphate buffer, pH 6.0. 50% loss of activity after 60 min in 0.1 M potassium phosphate buffer, pH 7.0, 50% loss of activity after 28 h in 0.1 M Tris-HCl buffer, pH 8.0. 50% loss of activity after 16 h in 0.1 M Tris-HCl buffer, pH 9.0 | Phlebiopsis gigantea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | 9 | - |
Phlebiopsis gigantea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
22°C, 50% loss of activity after 10 min | Phlebiopsis gigantea |
7 | - |
22°C, 50% loss of activity after 60 min | Phlebiopsis gigantea |
8 | - |
22°C, 50% loss of activity after 28 h | Phlebiopsis gigantea |
9 | - |
22°C, 50% loss of activity after 10 min | Phlebiopsis gigantea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | covalently bound | Phlebiopsis gigantea | |
FAD | 1 mol FAD per mol of subunit | Phlebiopsis gigantea |