General Stability | Organism |
---|---|
30°C, partially inactivated by 6 M urea or 3.5 M KBr after 1-2 h, complete inactivation in a mixture of theses agents | Ogataea angusta |
rapidly inactivated by freezing and thawing | Ogataea angusta |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,4-butynediol | irreversible | Ogataea angusta | |
4-Hydroxy-2-butynal | - |
Ogataea angusta | |
Cyclopropanol | - |
Ogataea angusta | |
Cyclopropanone | suicide substrate | Ogataea angusta | |
formaldehyde | - |
Ogataea angusta | |
H2O2 | 5-10 mM | Ogataea angusta | |
KBr | 3.5 M at 30°C for 1-2 h: partial inactivation, KBr + urea: complete inactivation | Ogataea angusta | |
KCN | - |
Ogataea angusta | |
NaN3 | - |
Ogataea angusta | |
PCMB | - |
Ogataea angusta | |
propargyl alcohol | irreversible | Ogataea angusta | |
Propynal | - |
Ogataea angusta | |
Urea | 6 M, at 30°C for 1-2 h: partial inactivation, urea + KBr: complete inactivation | Ogataea angusta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
formaldehyde | - |
Ogataea angusta |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Ogataea angusta | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
600000 | - |
gel filtration | Ogataea angusta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methanol + O2 | Ogataea angusta | - |
formaldehyde + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ogataea angusta | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Ogataea angusta |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a primary alcohol + O2 = an aldehyde + H2O2 | ping-pong mechanism operates via a two-electron transfer from substrate to oxygen | Ogataea angusta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23.2 | - |
- |
Ogataea angusta |
Storage Stability | Organism |
---|---|
4°C, stable for at least 6 months | Ogataea angusta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-propen-1-ol + O2 | - |
Ogataea angusta | 2-propen-1-al + H2O2 | in vivo leads to cell intoxination | ? | |
methanol + O2 | - |
Ogataea angusta | formaldehyde + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | calculated from amino acid sequence derived from nucleotide sequence of a structural gene | Ogataea angusta |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
24 h stable | Ogataea angusta |
45 | - |
1 h, stable | Ogataea angusta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | - |
Ogataea angusta |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | stable | Ogataea angusta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | noncovalently bound | Ogataea angusta | |
FAD | 1 mol FAD per mol of subunit | Ogataea angusta |