Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.3.13 extracted from

  • Van der Klei, I.J.; Bystryck, L.V.; Harder, W.
    Alcohol oxidase from Hansenula polymorpha CBS 4732 (1990), Methods Enzymol., 188, 420-427.
    View publication on PubMed

General Stability

General Stability Organism
30°C, partially inactivated by 6 M urea or 3.5 M KBr after 1-2 h, complete inactivation in a mixture of theses agents Ogataea angusta
rapidly inactivated by freezing and thawing Ogataea angusta

Inhibitors

Inhibitors Comment Organism Structure
1,4-butynediol irreversible Ogataea angusta
4-Hydroxy-2-butynal
-
Ogataea angusta
Cyclopropanol
-
Ogataea angusta
Cyclopropanone suicide substrate Ogataea angusta
formaldehyde
-
Ogataea angusta
H2O2 5-10 mM Ogataea angusta
KBr 3.5 M at 30°C for 1-2 h: partial inactivation, KBr + urea: complete inactivation Ogataea angusta
KCN
-
Ogataea angusta
NaN3
-
Ogataea angusta
PCMB
-
Ogataea angusta
propargyl alcohol irreversible Ogataea angusta
Propynal
-
Ogataea angusta
Urea 6 M, at 30°C for 1-2 h: partial inactivation, urea + KBr: complete inactivation Ogataea angusta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6
-
formaldehyde
-
Ogataea angusta

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
Ogataea angusta 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
600000
-
gel filtration Ogataea angusta

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + O2 Ogataea angusta
-
formaldehyde + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Ogataea angusta
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Ogataea angusta

Reaction

Reaction Comment Organism Reaction ID
a primary alcohol + O2 = an aldehyde + H2O2 ping-pong mechanism operates via a two-electron transfer from substrate to oxygen Ogataea angusta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.2
-
-
Ogataea angusta

Storage Stability

Storage Stability Organism
4°C, stable for at least 6 months Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-propen-1-ol + O2
-
Ogataea angusta 2-propen-1-al + H2O2 in vivo leads to cell intoxination ?
methanol + O2
-
Ogataea angusta formaldehyde + H2O2
-
?

Subunits

Subunits Comment Organism
octamer calculated from amino acid sequence derived from nucleotide sequence of a structural gene Ogataea angusta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
24 h stable Ogataea angusta
45
-
1 h, stable Ogataea angusta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 8.5
-
Ogataea angusta

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.5 stable Ogataea angusta

Cofactor

Cofactor Comment Organism Structure
FAD noncovalently bound Ogataea angusta
FAD 1 mol FAD per mol of subunit Ogataea angusta