Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Trametes sp. |
Protein Variants | Comment | Organism |
---|---|---|
E539K | increase in catalytic activity, shift in optimum temperature by 10 degrees | Trametes sp. |
K312E | increase in catalytic activity | Trametes sp. |
K312E/E539K | increase in catalytic activity, shift in optimum temperature by 10 degrees | Trametes sp. |
T166A/E539K | increase in catalytic activity | Trametes sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 5 mM, 95% of initial activity | Trametes sp. | |
EDTA | 5 mM, 85% of initial activity | Trametes sp. | |
Fe2+ | 5 mM, 11% of initial activity | Trametes sp. | |
SDS | 5 mM, complete loss of activity | Trametes sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
D-glucose | mutant T166A/E539K, pH 6.5, 30°C | Trametes sp. | |
0.6 | - |
D-glucose | mutant K312E/E539K, pH 6.5, 30°C | Trametes sp. | |
0.7 | - |
D-glucose | mutant E359K, pH 6.5, 30°C | Trametes sp. | |
0.8 | - |
D-glucose | mutant K312E, pH 6.5, 30°C | Trametes sp. | |
1.2 | - |
D-glucose | wild-type, pH 6.5, 30°C | Trametes sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 5 mM, 110% of initial activity | Trametes sp. | |
Mg2+ | 5 mM, 113% of initial activity | Trametes sp. | |
Mn2+ | 5 mM, 113% of initial activity | Trametes sp. | |
Na+ | 5 mM, 109% of initial activity | Trametes sp. | |
Ni2+ | 5 mM, 119% of initial activity | Trametes sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes sp. | A0A2P1MAF4 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.4 | - |
mutant T166A/E539K, pH 6.5, 30°C | Trametes sp. |
5.3 | - |
mutant E359K, pH 6.5, 30°C | Trametes sp. |
6.2 | - |
mutant K312E/E539K, pH 6.5, 30°C | Trametes sp. |
6.4 | - |
wild-type, pH 6.5, 30°C | Trametes sp. |
10.2 | - |
mutant K312E, pH 6.5, 30°C | Trametes sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + O2 | - |
Trametes sp. | 2-dehydro-D-glucose + H2O2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
wild-type and mutants K312E, T166A/E539K | Trametes sp. |
60 | - |
mutant E359K, K312E/E539K | Trametes sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
D-glucose | mutant T166A/E539K, pH 6.5, 30°C | Trametes sp. | |
6.1 | - |
D-glucose | wild-type, pH 6.5, 30°C | Trametes sp. | |
7.6 | - |
D-glucose | mutant E359K, pH 6.5, 30°C | Trametes sp. | |
7.6 | - |
D-glucose | mutant K312E/E539K, pH 6.5, 30°C | Trametes sp. | |
8.5 | - |
D-glucose | mutant K312E, pH 6.5, 30°C | Trametes sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
mutant K312E | Trametes sp. |
6 | - |
mutants E359K, K312E/E539K, T166A/E539K | Trametes sp. |
6.5 | - |
wiild-type | Trametes sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.08 | - |
D-glucose | wild-type, pH 6.5, 30°C | Trametes sp. | |
5.8 | - |
D-glucose | mutant T166A/E539K, pH 6.5, 30°C | Trametes sp. | |
10.62 | - |
D-glucose | mutant K312E, pH 6.5, 30°C | Trametes sp. | |
10.86 | - |
D-glucose | mutant E359K, pH 6.5, 30°C | Trametes sp. | |
12.67 | - |
D-glucose | mutant K312E/E539K, pH 6.5, 30°C | Trametes sp. |