KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.74 | - |
D-glucose | at pH 6.5 and 25°C | Irpex lacteus | |
7.73 | - |
D-galactose | at pH 6.5 and 25°C | Irpex lacteus | |
25.9 | - |
D-xylose | at pH 6.5 and 25°C | Irpex lacteus | |
39.3 | - |
L-sorbose | at pH 6.5 and 25°C | Irpex lacteus | |
46.4 | - |
maltose | at pH 6.5 and 25°C | Irpex lacteus | |
51.1 | - |
D-glucono-1,5-lactone | at pH 6.5 and 25°C | Irpex lacteus | |
290 | - |
cellobiose | at pH 6.5 and 25°C | Irpex lacteus | |
370 | - |
D-mannose | at pH 6.5 and 25°C | Irpex lacteus | |
727 | - |
D-fructose | at pH 6.5 and 25°C | Irpex lacteus | |
902 | - |
sucrose | at pH 6.5 and 25°C | Irpex lacteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
- |
Irpex lacteus |
266000 | - |
gel filtration | Irpex lacteus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + O2 | Irpex lacteus | preferred substrate | 2-dehydro-D-glucose + H2O2 | - |
? | |
D-glucose + O2 | Irpex lacteus dft-1 | preferred substrate | 2-dehydro-D-glucose + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Irpex lacteus | - |
- |
- |
Irpex lacteus dft-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phenyl Sepharose 6 column chromatography, DEAE-Sepharose column chromatography, and Sephacryl S-300 gel filtration | Irpex lacteus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Irpex lacteus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.31 | - |
unpurified enzyme, at pH 6.5 and 25°C | Irpex lacteus |
8.64 | - |
after 27.9fold purification, at pH 6.5 and 25°C | Irpex lacteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + O2 | 17.8% activity compared to D-glucose | Irpex lacteus | 2-dehydrocellobiose + H2O2 | - |
? | |
D-fructose + O2 | 12.4% activity compared to D-glucose | Irpex lacteus | 2-dehydro-D-fructose + H2O2 | - |
? | |
D-galactose + O2 | 38.8% activity compared to D-glucose | Irpex lacteus | 2-dehydro-D-galactose + H2O2 | - |
? | |
D-galactose + O2 | 38.8% activity compared to D-glucose | Irpex lacteus dft-1 | 2-dehydro-D-galactose + H2O2 | - |
? | |
D-glucono-1,5-lactone + O2 | 107% activity compared to D-glucose | Irpex lacteus | 2-dehydro-D-glucono-1,5-lactone + H2O2 | - |
? | |
D-glucose + 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) | - |
Irpex lacteus | 2-dehydro-D-glucose + ? | - |
? | |
D-glucose + O2 | preferred substrate | Irpex lacteus | 2-dehydro-D-glucose + H2O2 | - |
? | |
D-glucose + O2 | 100% activity | Irpex lacteus | 2-dehydro-D-glucose + H2O2 | - |
? | |
D-glucose + O2 | preferred substrate | Irpex lacteus dft-1 | 2-dehydro-D-glucose + H2O2 | - |
? | |
D-glucose + O2 | 100% activity | Irpex lacteus dft-1 | 2-dehydro-D-glucose + H2O2 | - |
? | |
D-mannose + O2 | 18.4% activity compared to D-glucose | Irpex lacteus | 2-dehydro-D-mannose + H2O2 | - |
? | |
D-xylose + O2 | 75.1% activity compared to D-glucose | Irpex lacteus | 2-dehydro-D-xylose + H2O2 | - |
? | |
L-sorbose + O2 | 122% activity compared to D-glucose | Irpex lacteus | 5-dehydro-D-fructose + H2O2 | - |
? | |
L-sorbose + O2 | 122% activity compared to D-glucose | Irpex lacteus dft-1 | 5-dehydro-D-fructose + H2O2 | - |
? | |
lactose + O2 | 0.71% activity compared to D-glucose | Irpex lacteus | 2-dehydrolactose + H2O2 | - |
? | |
maltose + O2 | 92.2% activity compared to D-glucose | Irpex lacteus | 2-dehydro-D-maltose + H2O2 | - |
? | |
additional information | no activity with D-arabinose | Irpex lacteus | ? | - |
? | |
additional information | no activity with D-arabinose | Irpex lacteus dft-1 | ? | - |
? | |
sucrose + O2 | 82.8% activity compared to D-glucose | Irpex lacteus | ? + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 71000, SDS-PAGE | Irpex lacteus |
Synonyms | Comment | Organism |
---|---|---|
C-2 specific pyranose-2-oxidase | - |
Irpex lacteus |
P2O | - |
Irpex lacteus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Irpex lacteus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 65 | the enzyme shows less than half of its maximum activity at 30°C and 65°C, respectively | Irpex lacteus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 80 | the enzyme remains stable up to 60°C. At 70°C, more than half of its initial activity is retained after 30 min of incubation in phosphate buffer, pH 6.5. At 80°C, it is totally inactivated within 30 min | Irpex lacteus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.19 | - |
D-fructose | at pH 6.5 and 25°C | Irpex lacteus | |
6.01 | - |
cellobiose | at pH 6.5 and 25°C | Irpex lacteus | |
6.2 | - |
D-mannose | at pH 6.5 and 25°C | Irpex lacteus | |
13.1 | - |
D-galactose | at pH 6.5 and 25°C | Irpex lacteus | |
25.4 | - |
D-xylose | at pH 6.5 and 25°C | Irpex lacteus | |
27.9 | - |
sucrose | at pH 6.5 and 25°C | Irpex lacteus | |
31.1 | - |
maltose | at pH 6.5 and 25°C | Irpex lacteus | |
33.8 | - |
D-glucose | at pH 6.5 and 25°C | Irpex lacteus | |
36.1 | - |
D-glucono-1,5-lactone | at pH 6.5 and 25°C | Irpex lacteus | |
41.4 | - |
L-sorbose | at pH 6.5 and 25°C | Irpex lacteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Irpex lacteus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | at pH 5.0 in citrate buffer and pH 9.0 in glycine-NaOH buffer, the enzyme displays about 60% of its maximum activity. The activity begins to decline dramatically below pH 5.0 and above pH 9.0 | Irpex lacteus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 11 | almost no activity loss occurs in the pH range 5.0-11.0 on incubation for 24 h at 25°C. Nearly half and 80% of its initial activity remains at pH 3.0 and 3.5, respectively | Irpex lacteus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | - |
D-fructose | at pH 6.5 and 25°C | Irpex lacteus | |
0.017 | - |
D-mannose | at pH 6.5 and 25°C | Irpex lacteus | |
0.021 | - |
cellobiose | at pH 6.5 and 25°C | Irpex lacteus | |
0.031 | - |
sucrose | at pH 6.5 and 25°C | Irpex lacteus | |
0.67 | - |
maltose | at pH 6.5 and 25°C | Irpex lacteus | |
0.71 | - |
D-glucono-1,5-lactone | at pH 6.5 and 25°C | Irpex lacteus | |
0.98 | - |
D-xylose | at pH 6.5 and 25°C | Irpex lacteus | |
1.05 | - |
L-sorbose | at pH 6.5 and 25°C | Irpex lacteus | |
1.7 | - |
D-galactose | at pH 6.5 and 25°C | Irpex lacteus | |
45.6 | - |
D-glucose | at pH 6.5 and 25°C | Irpex lacteus |