Any feedback?
Literature summary for 1.1.3.10 extracted from
- Regioselective control of beta-D-glucose oxidation by pyranose 2-oxidase is intimately coupled to conformational degeneracy (2011), J. Mol. Biol., 409, 588-600.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| P2O H167A mutant in complex with 3-deoxy-3-fluoro-D-glucose, microseeded hanging drops, mixing of equal volumes of 0.1 M MES, pH 5.2, 50 mM MgCl2, 10% w/v PEG monomethyl ether 2000 with 20 mg/ml protein in 20 mM MES, pH 5.2, addition of ligand in saturated mother liquor with 28% w/v PEG 2000, X-ray diffraction structure determination and analysis at 1.35 A resolution | Trametes ochracea |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D452A/R472A | the mutant shows drastic effects on the binding constant for D-glucose | Trametes ochracea |
| H167A | the P2O mutant in complex with the slow substrate 2-deoxy-2-fluoro-D-glucose shows a substrate-binding mode compatible with oxidation at C3. To accommodate the sugar, a gating segment, 454FSY456, in the substrate recognition loop partly unfolded to create a spacious and more polar active site that is distinct from the closed state of P2O. With 3-deoxy-3-fluoro-D-glucose the mutant prefers C2 oxidation, crystal structure of the ordered complex of P2O H167A with at 1.35 A resolution, overview | Trametes ochracea |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | hyphal periplasmic space | Trametes ochracea | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 270000 | - |
- |
Trametes ochracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + O2 | Trametes ochracea | highly regioselective mechanism | 2-dehydro-D-glucose + H2O2 | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes ochracea | Q7ZA32 | - |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| D-glucose + O2 = 2-dehydro-D-glucose + H2O2 | highly regioselective mechanism, overview. The different conformations of the 454FSY456 gating segment in the semiopen and closed states induce backbone and side-chain movements of Thr169 and Asp452 that add further differential stabilization to the individual states | Trametes ochracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose + O2 | highly regioselective mechanism | Trametes ochracea | 2-dehydro-D-glucose + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | - |
Trametes ochracea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| P2O | - |
Trametes ochracea |
| pyranose 2-Oxidase | - |
Trametes ochracea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | dependent on | Trametes ochracea | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | substrate recognition loop, active-site protein interactions for D-glucose, and structural determinants of the productive glucose C2-oxidation mode, detailed overview | Trametes ochracea |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
