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Literature summary for 1.1.3.10 extracted from

  • Decamps, K.; Joye, I.; Haltrich, D.; Nicolas, J.; Courtin, C.; Delcour, J.
    Biochemical characteristics of Trametes multicolor pyranose oxidase and Aspergillus niger glucose oxidase and implications for their functionality in wheat flour dough (2012), Food Chem., 131, 1485-1492.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Trametes ochracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics, overview Trametes ochracea
0.46
-
O2 pH and temperature not specified in the publication Trametes ochracea
3.1
-
D-glucose pH and temperature not specified in the publication Trametes ochracea
78.4
-
D-xylose pH and temperature not specified in the publication Trametes ochracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose + O2 Trametes ochracea
-
2-dehydro-D-glucose + H2O2
-
?
D-glucose + O2 Trametes ochracea MB49
-
2-dehydro-D-glucose + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Trametes ochracea Q7ZA32
-
-
Trametes ochracea MB49 Q7ZA32
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinit chromatography and ulrafiltration Trametes ochracea

Reaction

Reaction Comment Organism Reaction ID
D-glucose + O2 = 2-dehydro-D-glucose + H2O2 the enzyme shows a ping pong bi bi mechanism, a specialised bi bi mechanism in which substrate binding and release of products are ordered and the enzyme shuttles between a free and a substrate-modified intermediate conformation Trametes ochracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + O2
-
Trametes ochracea 2-dehydro-D-glucose + H2O2
-
?
D-glucose + O2
-
Trametes ochracea MB49 2-dehydro-D-glucose + H2O2
-
?
D-xylose + O2
-
Trametes ochracea 2-dehydro-D-xylose + H2O2
-
?
D-xylose + O2
-
Trametes ochracea MB49 2-dehydro-D-xylose + H2O2
-
?
additional information ferulic acid oxidation in a model system and of thiol oxidation in a wheat flour extract, overview. L-arabinose is a poor substrate. Dehydroascorbic acid does not act as electron acceptor Trametes ochracea ?
-
?
additional information ferulic acid oxidation in a model system and of thiol oxidation in a wheat flour extract, overview. L-arabinose is a poor substrate. Dehydroascorbic acid does not act as electron acceptor Trametes ochracea MB49 ?
-
?

Synonyms

Synonyms Comment Organism
P2O
-
Trametes ochracea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Trametes ochracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6.5
-
Trametes ochracea

Cofactor

Cofactor Comment Organism Structure
FAD
-
Trametes ochracea