Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Trametes ochracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, overview | Trametes ochracea | |
0.46 | - |
O2 | pH and temperature not specified in the publication | Trametes ochracea | |
3.1 | - |
D-glucose | pH and temperature not specified in the publication | Trametes ochracea | |
78.4 | - |
D-xylose | pH and temperature not specified in the publication | Trametes ochracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + O2 | Trametes ochracea | - |
2-dehydro-D-glucose + H2O2 | - |
? | |
D-glucose + O2 | Trametes ochracea MB49 | - |
2-dehydro-D-glucose + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes ochracea | Q7ZA32 | - |
- |
Trametes ochracea MB49 | Q7ZA32 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinit chromatography and ulrafiltration | Trametes ochracea |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-glucose + O2 = 2-dehydro-D-glucose + H2O2 | the enzyme shows a ping pong bi bi mechanism, a specialised bi bi mechanism in which substrate binding and release of products are ordered and the enzyme shuttles between a free and a substrate-modified intermediate conformation | Trametes ochracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose + O2 | - |
Trametes ochracea | 2-dehydro-D-glucose + H2O2 | - |
? | |
D-glucose + O2 | - |
Trametes ochracea MB49 | 2-dehydro-D-glucose + H2O2 | - |
? | |
D-xylose + O2 | - |
Trametes ochracea | 2-dehydro-D-xylose + H2O2 | - |
? | |
D-xylose + O2 | - |
Trametes ochracea MB49 | 2-dehydro-D-xylose + H2O2 | - |
? | |
additional information | ferulic acid oxidation in a model system and of thiol oxidation in a wheat flour extract, overview. L-arabinose is a poor substrate. Dehydroascorbic acid does not act as electron acceptor | Trametes ochracea | ? | - |
? | |
additional information | ferulic acid oxidation in a model system and of thiol oxidation in a wheat flour extract, overview. L-arabinose is a poor substrate. Dehydroascorbic acid does not act as electron acceptor | Trametes ochracea MB49 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
P2O | - |
Trametes ochracea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Trametes ochracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6.5 | - |
Trametes ochracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Trametes ochracea |