Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.2.4 extracted from

  • de Bari, L.; Moro, L.; Passarella, S.
    Prostate cancer cells metabolize D-lactate inside mitochondria via a D-lactate dehydrogenase which is more active and highly expressed than in normal cells (2013), FEBS Lett., 587, 467-473.
    View publication on PubMed
Search for more literature for 1.1.2.4

Inhibitors

Inhibitors Comment Organism Structure
oxalate
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial inner membrane the enzyme is located on the inner face of the inner mitochondrial membrane Homo sapiens 5743
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-lactate + 2 ferricytochrome c Homo sapiens
-
 pyruvate + 2 ferrocytochrome c
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q86WU2
-
-

Source Tissue

Source Tissue Comment Organism Textmining
PC-3 cell
-
 Homo sapiens
-
PNT-1A cell
-
 Homo sapiens
-
prostate cancer cell
-
 Homo sapiens
-
prostate gland
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-lactate + 2 ferricytochrome c
-
 Homo sapiens pyruvate + 2 ferrocytochrome c
-
 ?
additional information D-LDH activity assay and (R)-lactate-dependent cyt c and flavin reduction Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
D-LDH
-
 Homo sapiens
DLD
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD the enzyme is a flavoprotein. The flavin/pyridine cofactor redox state is detected fluorimetrically in cell homogenates Homo sapiens
ferricytochrome c
-
 Homo sapiens

General Information

General Information Comment Organism
metabolism prostate cancer cells metabolize D-lactate inside mitochondria via a D-lactate dehydrogenase which is more active and highly expressed than in normal cells. D-Lactate can enter prostate mitochondria and cause the export of newly synthesized malate in a carrier-mediated manner, with the rate of malate efflux from mitochondria being twofold higher in cancer compared to healthy cells, overview Homo sapiens
physiological function the mitochondrial D-lactate dehydrogenase, a membrane flavoprotein, from both cancer (PC-3) and normal (PNT1A) prostate cells can metabolize D-lactate in an energy competent manner Homo sapiens