Application | Comment | Organism |
---|---|---|
analysis | miniaturized assay for quantitative high-throughput screening, by monitoring NADPH oxidation as a decrease in A340 using a spectrophotometer | Pyrobaculum aerophilum |
Crystallization (Comment) | Organism |
---|---|
crystal structure of both wild-type and cobalt-substituted enzyme at 1.75 A and 2.20 A resolution, respectively. One metal ion per monomer is present only at the structural site. The co-crystal structure of the NADPH-bound form of the enzyme at 2.35 A resolution shows close structural conservation with horse ADH, despite the lack of a catalytic Zn2+. Modeling of 1-tetralone into the NADPH-bound structure suggests an arginine as a possible catalytic residue | Pyrobaculum aerophilum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-[2-(diphenylmethoxy)ethyl]-4-[(2E)-3-phenylprop-2-en-1-yl]piperazine | i.e. GBR12783 | Pyrobaculum aerophilum | |
1-[2-[bis(4-fluorophenyl)methoxy]ethyl]-4-[(2E)-3-phenylprop-2-en-1-yl]piperazine | - |
Pyrobaculum aerophilum | |
1-[bis(4-fluorophenyl)methyl]-4-[(2E)-3-phenylprop-2-en-1-yl]piperazine | i.e. flunarizine | Pyrobaculum aerophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum aerophilum | Q8ZUP0 | - |
- |
Pyrobaculum aerophilum DSM 7523 | Q8ZUP0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-tetralone + NADPH + H+ | - |
Pyrobaculum aerophilum | 1-tetralol + NADP+ | - |
? | |
1-tetralone + NADPH + H+ | - |
Pyrobaculum aerophilum DSM 7523 | 1-tetralol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADHII | - |
Pyrobaculum aerophilum |
PAE2687 | - |
Pyrobaculum aerophilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Pyrobaculum aerophilum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.01 | - |
pH 7.5, 37°C | Pyrobaculum aerophilum | 1-[2-[bis(4-fluorophenyl)methoxy]ethyl]-4-[(2E)-3-phenylprop-2-en-1-yl]piperazine | |
0.04 | - |
pH 7.5, 37°C | Pyrobaculum aerophilum | 1-[bis(4-fluorophenyl)methyl]-4-[(2E)-3-phenylprop-2-en-1-yl]piperazine | |
0.04 | - |
pH 7.5, 37°C | Pyrobaculum aerophilum | 1-[2-(diphenylmethoxy)ethyl]-4-[(2E)-3-phenylprop-2-en-1-yl]piperazine |