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show all sequences of 1.1.1.B57

Properties and evolution of an alcohol dehydrogenase from the Crenarchaeota Pyrobaculum aerophilum

Vitale, A.; Rosso, F.; Barbarisi, A.; Labella, T.; D'Auria, S.; Gene 461, 26-31 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression in Escherichia coli
Pyrobaculum aerophilum
General Stability
General Stability
Organism
Zn2+ is not essential for structural stability of the enzyme
Pyrobaculum aerophilum
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
1 mM, 20% decrease in activity
Pyrobaculum aerophilum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4
-
3,4-dihydro-1(2H)-naphthalenone
95C, pH 7.5
Pyrobaculum aerophilum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
important for activity
Pyrobaculum aerophilum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38580
-
x * 38580, calculated from sequence
Pyrobaculum aerophilum
40000
-
x * 40000, SDS-PAGE
Pyrobaculum aerophilum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pyrobaculum aerophilum
Q8ZUP0
-
-
Pyrobaculum aerophilum DSM 7523
Q8ZUP0
-
-
Purification (Commentary)
Commentary
Organism
-
Pyrobaculum aerophilum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3
-
60C, pH 7.5
Pyrobaculum aerophilum
50
-
95C, pH 7.5
Pyrobaculum aerophilum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+
3,4-dihydro-1(2H)-naphthalenone i.e. alpha-tetralone. The enzyme does not show activity on a large number of alcohols, aldehydes or ketones. It is active only with alpha-tetralone as substrate
727585
Pyrobaculum aerophilum
1,2,3,4-tetrahydronaphthalen-1-ol + NADP+
-
-
-
?
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+
3,4-dihydro-1(2H)-naphthalenone i.e. alpha-tetralone. The enzyme does not show activity on a large number of alcohols, aldehydes or ketones. It is active only with alpha-tetralone as substrate
727585
Pyrobaculum aerophilum DSM 7523
1,2,3,4-tetrahydronaphthalen-1-ol + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 38580, calculated from sequence; x * 40000, SDS-PAGE
Pyrobaculum aerophilum
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
95
-
reaction rate increases with temperature up to 95C
Pyrobaculum aerophilum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1719
-
3,4-dihydro-1(2H)-naphthalenone
95C, pH 7.5
Pyrobaculum aerophilum
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Escherichia coli
Pyrobaculum aerophilum
General Stability (protein specific)
General Stability
Organism
Zn2+ is not essential for structural stability of the enzyme
Pyrobaculum aerophilum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
1 mM, 20% decrease in activity
Pyrobaculum aerophilum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4
-
3,4-dihydro-1(2H)-naphthalenone
95C, pH 7.5
Pyrobaculum aerophilum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
important for activity
Pyrobaculum aerophilum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38580
-
x * 38580, calculated from sequence
Pyrobaculum aerophilum
40000
-
x * 40000, SDS-PAGE
Pyrobaculum aerophilum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pyrobaculum aerophilum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
3
-
60C, pH 7.5
Pyrobaculum aerophilum
50
-
95C, pH 7.5
Pyrobaculum aerophilum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+
3,4-dihydro-1(2H)-naphthalenone i.e. alpha-tetralone. The enzyme does not show activity on a large number of alcohols, aldehydes or ketones. It is active only with alpha-tetralone as substrate
727585
Pyrobaculum aerophilum
1,2,3,4-tetrahydronaphthalen-1-ol + NADP+
-
-
-
?
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+
3,4-dihydro-1(2H)-naphthalenone i.e. alpha-tetralone. The enzyme does not show activity on a large number of alcohols, aldehydes or ketones. It is active only with alpha-tetralone as substrate
727585
Pyrobaculum aerophilum DSM 7523
1,2,3,4-tetrahydronaphthalen-1-ol + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 38580, calculated from sequence; x * 40000, SDS-PAGE
Pyrobaculum aerophilum
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
95
-
reaction rate increases with temperature up to 95C
Pyrobaculum aerophilum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1719
-
3,4-dihydro-1(2H)-naphthalenone
95C, pH 7.5
Pyrobaculum aerophilum
Other publictions for EC 1.1.1.B57
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728605
Vitale
Physicochemical characterizati ...
Pyrobaculum aerophilum, Pyrobaculum aerophilum DSM 7523
PLoS One
8
e63828
2013
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1
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1
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3
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4
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2
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1
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3
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1
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1
1
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3
3
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-
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2
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727585
Vitale
Properties and evolution of an ...
Pyrobaculum aerophilum, Pyrobaculum aerophilum DSM 7523
Gene
461
26-31
2010
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1
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1
1
1
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1
2
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4
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1
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2
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2
1
1
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1
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1
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1
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1
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1
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1
2
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1
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2
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2
1
1
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1
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