BRENDA - Enzyme Database show
show all sequences of 1.1.1.B52

Engineering of highly selective variants of Parvibaculum lavamentivorans alcohol dehydrogenase

Spickermann, D.; Hausmann, S.; Degering, C.; Schwaneberg, U.; Leggewie, C.; ChemBioChem 15, 2050-2052 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Parvibaculum lavamentivorans
Engineering
Amino acid exchange
Commentary
Organism
A158A/N162P
96.8% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion
Parvibaculum lavamentivorans
A158D/N162G
96.5% enantiomeric excess for (R)-3-quinuclidinol production, 87% conversion
Parvibaculum lavamentivorans
A158D/N162Q
95.8% enantiomeric excess for (R)-3-quinuclidinol production, 79% conversion
Parvibaculum lavamentivorans
A158H/N162G/K202Q/L224W
mutations increase the enantiomeric excess for (R)-3-quinuclidinol production from 84.3% (wild-type) to 99% and concomitantly to enhance conversion by 43.5%
Parvibaculum lavamentivorans
A158H/N162P
98.1% enantiomeric excess for (R)-3-quinuclidinol production, 95% conversion
Parvibaculum lavamentivorans
A158K/N162M
97.6% enantiomeric excess for (R)-3-quinuclidinol production, 98% conversion
Parvibaculum lavamentivorans
K202N/L224M
89.7% enantiomeric excess for (R)-3-quinuclidinol production, 90% conversion
Parvibaculum lavamentivorans
K202Q/L224W
95.5% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion
Parvibaculum lavamentivorans
K202R/L224W
94.7% enantiomeric excess for (R)-3-quinuclidinol production, 91% conversion
Parvibaculum lavamentivorans
K202S/L224Y
89.5% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion
Parvibaculum lavamentivorans
N162A
93.8% enantiomeric excess for (R)-3-quinuclidinol production, 91% conversion
Parvibaculum lavamentivorans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Parvibaculum lavamentivorans
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-quinuclidinone + NADH + H+
-
738052
Parvibaculum lavamentivorans
(R)-3-quinuclidinol + NAD+
84% ee, 62% conversion after 22 h
-
-
?
additional information
enzyme is an alcohol dehydrogenase, reaction of EC 1.1.1.1
738052
Parvibaculum lavamentivorans
?
-
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Parvibaculum lavamentivorans
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
A158A/N162P
96.8% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion
Parvibaculum lavamentivorans
A158D/N162G
96.5% enantiomeric excess for (R)-3-quinuclidinol production, 87% conversion
Parvibaculum lavamentivorans
A158D/N162Q
95.8% enantiomeric excess for (R)-3-quinuclidinol production, 79% conversion
Parvibaculum lavamentivorans
A158H/N162G/K202Q/L224W
mutations increase the enantiomeric excess for (R)-3-quinuclidinol production from 84.3% (wild-type) to 99% and concomitantly to enhance conversion by 43.5%
Parvibaculum lavamentivorans
A158H/N162P
98.1% enantiomeric excess for (R)-3-quinuclidinol production, 95% conversion
Parvibaculum lavamentivorans
A158K/N162M
97.6% enantiomeric excess for (R)-3-quinuclidinol production, 98% conversion
Parvibaculum lavamentivorans
K202N/L224M
89.7% enantiomeric excess for (R)-3-quinuclidinol production, 90% conversion
Parvibaculum lavamentivorans
K202Q/L224W
95.5% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion
Parvibaculum lavamentivorans
K202R/L224W
94.7% enantiomeric excess for (R)-3-quinuclidinol production, 91% conversion
Parvibaculum lavamentivorans
K202S/L224Y
89.5% enantiomeric excess for (R)-3-quinuclidinol production, 82% conversion
Parvibaculum lavamentivorans
N162A
93.8% enantiomeric excess for (R)-3-quinuclidinol production, 91% conversion
Parvibaculum lavamentivorans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-quinuclidinone + NADH + H+
-
738052
Parvibaculum lavamentivorans
(R)-3-quinuclidinol + NAD+
84% ee, 62% conversion after 22 h
-
-
?
additional information
enzyme is an alcohol dehydrogenase, reaction of EC 1.1.1.1
738052
Parvibaculum lavamentivorans
?
-
-
-
-
Other publictions for EC 1.1.1.B52
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729146
Hou
Structural basis for high subs ...
Agrobacterium tumefaciens
Biochem. Biophys. Res. Commun.
446
911-915
2014
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2
1
1
4
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
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1
-
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1
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1
-
-
-
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2
1
1
1
4
1
-
-
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-
-
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-
-
1
-
-
-
-
-
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1
-
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-
1
-
-
-
-
-
-
-
-
-
738052
Spickermann
Engineering of highly selectiv ...
Parvibaculum lavamentivorans
ChemBioChem
15
2050-2052
2014
-
-
1
-
11
-
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1
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2
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1
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11
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2
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730545
Zhang
Highly efficient synthesis of ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens ECU2556
Org. Lett.
15
4917-4919
2013
-
2
1
-
-
-
-
1
-
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2
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2
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2
1
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1
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2
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1
1
728893
Hou
Expression, purification, crys ...
Agrobacterium tumefaciens
Acta Crystallogr. Sect. F
68
1237-1239
2012
-
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1
1
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-
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1
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1
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1
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1
1
1
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1
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729032
Isotani
Gene cloning and characterizat ...
Microbacterium luteolum, Microbacterium luteolum JCM 9174
Appl. Environ. Microbiol.
79
1378-1384
2012
-
2
1
-
-
-
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4
-
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4
-
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3
-
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1
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2
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8
2
1
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2
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1
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2
1
1
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4
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4
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1
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2
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8
2
1
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2
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729854
Isotani
Production of (R)-3-quinuclidi ...
Microbacterium luteolum, Microbacterium luteolum JCM 9174
Int. J. Mol. Sci.
13
13542-13553
2012
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2
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3
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2
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1
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1
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1
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2
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1
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2
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1
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1
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