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show all sequences of 1.1.1.B52

Structural basis for high substrate-binding affinity and enantioselectivity of 3-quinuclidinone reductase AtQR

Hou, F.; Miyakawa, T.; Kataoka, M.; Takeshita, D.; Kumashiro, S.; Uzura, A.; Urano, N.; Nagata, K.; Shimizu, S.; Tanokura, M.; Biochem. Biophys. Res. Commun. 446, 911-915 (2014)

Data extracted from this reference:

Application
Application
Commentary
Organism
pharmacology
stereospecific production of (R)-3-quinuclidinol, an important chiral building block for the synthesis of various pharmaceuticals
Agrobacterium tumefaciens
synthesis
stereospecific production of (R)-3-quinuclidinol, an important chiral building block for the synthesis of various pharmaceuticals
Agrobacterium tumefaciens
Cloned(Commentary)
Commentary
Organism
overexpressed as a fusion protein with an N-terminal His6 tag in Eschrichia coli Rosetta(DE3)
Agrobacterium tumefaciens
Crystallization (Commentary)
Crystallization
Organism
crystallized under a reservoir solution condition of 0.2 M ammonium acetate, 0.1 M HEPES pH 8.5 and 24% (w/v) PEG3350
Agrobacterium tumefaciens
Engineering
Amino acid exchange
Commentary
Organism
D40A
no activity
Agrobacterium tumefaciens
E197A
no activity
Agrobacterium tumefaciens
R196A
activity is 76% compared to activity of wild-type enzyme
Agrobacterium tumefaciens
Y216V
activity is 31% compared to activity of wild-type enzyme
Agrobacterium tumefaciens
General Stability
General Stability
Organism
NADH contributes to the stability of the alpha7 helix of the enzyme
Agrobacterium tumefaciens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Agrobacterium tumefaciens
G1K3P5
-
-
Purification (Commentary)
Commentary
Organism
-
Agrobacterium tumefaciens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Agrobacterium tumefaciens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Agrobacterium tumefaciens
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Agrobacterium tumefaciens
Application (protein specific)
Application
Commentary
Organism
pharmacology
stereospecific production of (R)-3-quinuclidinol, an important chiral building block for the synthesis of various pharmaceuticals
Agrobacterium tumefaciens
synthesis
stereospecific production of (R)-3-quinuclidinol, an important chiral building block for the synthesis of various pharmaceuticals
Agrobacterium tumefaciens
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpressed as a fusion protein with an N-terminal His6 tag in Eschrichia coli Rosetta(DE3)
Agrobacterium tumefaciens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Agrobacterium tumefaciens
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystallized under a reservoir solution condition of 0.2 M ammonium acetate, 0.1 M HEPES pH 8.5 and 24% (w/v) PEG3350
Agrobacterium tumefaciens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
D40A
no activity
Agrobacterium tumefaciens
E197A
no activity
Agrobacterium tumefaciens
R196A
activity is 76% compared to activity of wild-type enzyme
Agrobacterium tumefaciens
Y216V
activity is 31% compared to activity of wild-type enzyme
Agrobacterium tumefaciens
General Stability (protein specific)
General Stability
Organism
NADH contributes to the stability of the alpha7 helix of the enzyme
Agrobacterium tumefaciens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Agrobacterium tumefaciens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Agrobacterium tumefaciens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Agrobacterium tumefaciens
Other publictions for EC 1.1.1.B52
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729146
Hou
Structural basis for high subs ...
Agrobacterium tumefaciens
Biochem. Biophys. Res. Commun.
446
911-915
2014
-
2
1
1
4
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
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-
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1
-
-
-
1
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1
-
-
-
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2
1
1
1
4
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
738052
Spickermann
Engineering of highly selectiv ...
Parvibaculum lavamentivorans
ChemBioChem
15
2050-2052
2014
-
-
1
-
11
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
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-
-
-
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-
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-
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1
-
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11
-
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-
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-
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-
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2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730545
Zhang
Highly efficient synthesis of ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens ECU2556
Org. Lett.
15
4917-4919
2013
-
2
1
-
-
-
-
1
-
-
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-
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2
-
-
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-
-
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2
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2
1
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1
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-
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-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
728893
Hou
Expression, purification, crys ...
Agrobacterium tumefaciens
Acta Crystallogr. Sect. F
68
1237-1239
2012
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
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1
-
-
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1
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1
1
1
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1
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-
-
-
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-
-
-
-
-
-
-
-
-
-
-
729032
Isotani
Gene cloning and characterizat ...
Microbacterium luteolum, Microbacterium luteolum JCM 9174
Appl. Environ. Microbiol.
79
1378-1384
2012
-
2
1
-
-
-
-
4
-
-
4
-
-
3
-
-
1
-
-
-
2
-
8
2
1
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2
-
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1
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2
1
1
-
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4
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4
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1
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2
-
8
2
1
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2
-
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-
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729854
Isotani
Production of (R)-3-quinuclidi ...
Microbacterium luteolum, Microbacterium luteolum JCM 9174
Int. J. Mol. Sci.
13
13542-13553
2012
-
2
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3
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2
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1
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1
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1
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1
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1
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