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show all sequences of 1.1.1.B52

Expression, purification, crystallization and X-ray analysis of 3-quinuclidinone reductase from Agrobacterium tumefaciens

Hou, F.; Miyakawa, T.; Takeshita, D.; Kataoka, M.; Uzura, A.; Nagata, K.; Shimizu, S.; Tanokura, M.; Acta Crystallogr. Sect. F 68, 1237-1239 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpressed in Escherichia coli
Agrobacterium tumefaciens
Crystallization (Commentary)
Crystallization
Organism
sitting-drop vapour-diffusion method at 20°C. Crystals are obtained using a reservoir solution containing PEG 3350 as a precipitant. X-ray diffraction data are collected to 1.72 A resolution. The crystal belongs to space group P2(1), with unit-cell parameters a = 62.0, b = 126.4, c = 62.0 A, beta = 110.5, and is suggested to contain four molecules in the asymmetric unit
Agrobacterium tumefaciens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Agrobacterium tumefaciens
G1K3P5
-
-
Purification (Commentary)
Commentary
Organism
-
Agrobacterium tumefaciens
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Agrobacterium tumefaciens
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpressed in Escherichia coli
Agrobacterium tumefaciens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Agrobacterium tumefaciens
Crystallization (Commentary) (protein specific)
Crystallization
Organism
sitting-drop vapour-diffusion method at 20°C. Crystals are obtained using a reservoir solution containing PEG 3350 as a precipitant. X-ray diffraction data are collected to 1.72 A resolution. The crystal belongs to space group P2(1), with unit-cell parameters a = 62.0, b = 126.4, c = 62.0 A, beta = 110.5, and is suggested to contain four molecules in the asymmetric unit
Agrobacterium tumefaciens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Agrobacterium tumefaciens
Other publictions for EC 1.1.1.B52
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729146
Hou
Structural basis for high subs ...
Agrobacterium tumefaciens
Biochem. Biophys. Res. Commun.
446
911-915
2014
-
2
1
1
4
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
2
1
1
1
4
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
738052
Spickermann
Engineering of highly selectiv ...
Parvibaculum lavamentivorans
ChemBioChem
15
2050-2052
2014
-
-
1
-
11
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730545
Zhang
Highly efficient synthesis of ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens ECU2556
Org. Lett.
15
4917-4919
2013
-
2
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
728893
Hou
Expression, purification, crys ...
Agrobacterium tumefaciens
Acta Crystallogr. Sect. F
68
1237-1239
2012
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729032
Isotani
Gene cloning and characterizat ...
Microbacterium luteolum, Microbacterium luteolum JCM 9174
Appl. Environ. Microbiol.
79
1378-1384
2012
-
2
1
-
-
-
-
4
-
-
4
-
-
3
-
-
1
-
-
-
2
-
8
2
1
-
-
-
2
-
-
1
-
-
-
-
2
1
1
-
-
-
-
-
-
4
-
-
4
-
-
-
-
1
-
-
2
-
8
2
1
-
-
-
2
-
-
-
-
-
-
-
-
-
729854
Isotani
Production of (R)-3-quinuclidi ...
Microbacterium luteolum, Microbacterium luteolum JCM 9174
Int. J. Mol. Sci.
13
13542-13553
2012
-
2
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-