BRENDA - Enzyme Database show
show all sequences of 1.1.1.B51

Crystallization and preliminary X-ray analysis of the NADPH-dependent 3-quinuclidinone reductase from Rhodotorula rubra

Takeshita, D.; Kataoka, M.; Miyakawa, T.; Miyazono, K.; Uzura, A.; Nagata, K.; Shimizu, S.; Tanokura, M.; Acta Crystallogr. Sect. F 65, 645-647 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Rhodotorula mucilaginosa
Crystallization (Commentary)
Crystallization
Organism
sitting-drop vapour-diffusion method using PEG 8000 as the precipitant. The crystals belonged to space group P4(1)2(1)2, with unit-cell parameters a = b = 91.3, c = 265.4 A, and diffracted X-rays to 2.2 A resolution.The asymmetric unit contained four molecules of the protein and the solvent content was 48.4%
Rhodotorula mucilaginosa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodotorula mucilaginosa
B9ZZZ6
-
-
Purification (Commentary)
Commentary
Organism
Ni-affinity and ion-exchange column chromatography
Rhodotorula mucilaginosa
Subunits
Subunits
Commentary
Organism
tetramer
crystallographic data
Rhodotorula mucilaginosa
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Rhodotorula mucilaginosa
Crystallization (Commentary) (protein specific)
Crystallization
Organism
sitting-drop vapour-diffusion method using PEG 8000 as the precipitant. The crystals belonged to space group P4(1)2(1)2, with unit-cell parameters a = b = 91.3, c = 265.4 A, and diffracted X-rays to 2.2 A resolution.The asymmetric unit contained four molecules of the protein and the solvent content was 48.4%
Rhodotorula mucilaginosa
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-affinity and ion-exchange column chromatography
Rhodotorula mucilaginosa
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
crystallographic data
Rhodotorula mucilaginosa
Other publictions for EC 1.1.1.B51
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728943
Takeshita
Structural basis of stereospec ...
Rhodotorula mucilaginosa
AMB Express
4
0000
2014
-
-
-
1
11
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
1
-
-
5
1
-
-
1
-
-
-
-
-
-
1
1
11
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
-
-
5
1
-
-
-
-
-
-
-
-
-
728883
Takeshita
Crystallization and preliminar ...
Rhodotorula mucilaginosa
Acta Crystallogr. Sect. F
65
645-647
2009
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
729053
Uzura
Stereoselective synthesis of ( ...
Rhodotorula mucilaginosa, Rhodotorula mucilaginosa JCM3782
Appl. Microbiol. Biotechnol.
83
617-626
2009
-
2
1
-
-
-
3
2
-
-
2
-
-
2
-
-
1
-
-
-
1
-
8
1
1
-
-
-
2
-
-
1
-
-
-
-
2
1
1
-
-
-
-
3
-
2
-
-
2
-
-
-
-
1
-
-
1
-
8
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-