BRENDA - Enzyme Database show
show all sequences of 1.1.1.B47

Malonic semialdehyde reductase, succinic semialdehyde reductase, and succinyl-coenzyme A reductase from Metallosphaera sedula: Enzymes of the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle in Sulfolobales

Kockelkorn, D.; Fuchs, G.; J. Bacteriol. 191, 6352-6362 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli Rosetta 2(DE3)
Metallosphaera sedula
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition by malonic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde. Incubation with 10 mM EDTA for 16 h or with 100 mM EDTA for 1 h at temperatures up to 65°C does not inactivate the enzyme, nor does addition of 10 mM EDTA to the assay mixture inhibit enzyme activity
Metallosphaera sedula
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
NADPH
65°C, pH 7.9
Metallosphaera sedula
0.052
-
succinate semialdehyde
65°C, pH 7.9, cosubstrate: NADPH
Metallosphaera sedula
0.18
-
NADH
65°C, pH 7.9
Metallosphaera sedula
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no stimulation by Mg2+ or Mn2+ (below 5 mM), or Fe2+ (below 1 mM)
Metallosphaera sedula
Zn2+
the enzyme contains 1.9 mol of zinc per mol of enzyme monomer
Metallosphaera sedula
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Metallosphaera sedula
74000
-
gel filtration
Metallosphaera sedula
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinate semialdehyde + NADPH + H+
Metallosphaera sedula
the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea
4-hydroxybutanoate + NADP+
-
-
?
succinate semialdehyde + NADPH + H+
Metallosphaera sedula DSM 5348
the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea
4-hydroxybutanoate + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Metallosphaera sedula
A4YGN0
-
-
Metallosphaera sedula DSM 5348
A4YGN0
-
-
Purification (Commentary)
Commentary
Organism
-
Metallosphaera sedula
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culture condition:autotrophically-grown cell
no activity in heterotrophically-grown cell
Metallosphaera sedula
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.15
-
cell extract, NADH-dependent reaction, 65°C, pH 7.9
Metallosphaera sedula
1.4
-
cell extract, NADPH-dependent reaction, 65°C, pH 7.9
Metallosphaera sedula
700
-
purified enzyme, NADPH-dependent reaction, 65°C, pH 7.9
Metallosphaera sedula
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
malonic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde do not serve as a substrate
698628
Metallosphaera sedula
?
-
-
-
-
additional information
malonic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde do not serve as a substrate
698628
Metallosphaera sedula DSM 5348
?
-
-
-
-
succinate semialdehyde + NADH + H+
very low activity with NADH
698628
Metallosphaera sedula
4-hydroxybutanoate + NAD+
-
-
-
?
succinate semialdehyde + NADH + H+
very low activity with NADH
698628
Metallosphaera sedula DSM 5348
4-hydroxybutanoate + NAD+
-
-
-
?
succinate semialdehyde + NADPH + H+
-
698628
Metallosphaera sedula
4-hydroxybutanoate + NADP+
-
-
-
?
succinate semialdehyde + NADPH + H+
the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea
698628
Metallosphaera sedula
4-hydroxybutanoate + NADP+
-
-
-
?
succinate semialdehyde + NADPH + H+
-
698628
Metallosphaera sedula DSM 5348
4-hydroxybutanoate + NADP+
-
-
-
?
succinate semialdehyde + NADPH + H+
the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea
698628
Metallosphaera sedula DSM 5348
4-hydroxybutanoate + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 40000, SDS-PAGE
Metallosphaera sedula
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
90
-
-
Metallosphaera sedula
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
440
-
NADPH
65°C, pH 7.9
Metallosphaera sedula
440
-
succinate semialdehyde
65°C, pH 7.9
Metallosphaera sedula
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
at 20°C
Metallosphaera sedula
7.5
-
at 65°C
Metallosphaera sedula
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.5
8
at 65°C, half-maximal activity at pH 6.5 and at pH 8.0
Metallosphaera sedula
6.9
8.4
at 20°C, half-maximal activity at pH 6.9 and at pH 8.4
Metallosphaera sedula
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Metallosphaera sedula
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli Rosetta 2(DE3)
Metallosphaera sedula
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Metallosphaera sedula
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition by malonic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde. Incubation with 10 mM EDTA for 16 h or with 100 mM EDTA for 1 h at temperatures up to 65°C does not inactivate the enzyme, nor does addition of 10 mM EDTA to the assay mixture inhibit enzyme activity
Metallosphaera sedula
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
NADPH
65°C, pH 7.9
Metallosphaera sedula
0.052
-
succinate semialdehyde
65°C, pH 7.9, cosubstrate: NADPH
Metallosphaera sedula
0.18
-
NADH
65°C, pH 7.9
Metallosphaera sedula
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no stimulation by Mg2+ or Mn2+ (below 5 mM), or Fe2+ (below 1 mM)
Metallosphaera sedula
Zn2+
the enzyme contains 1.9 mol of zinc per mol of enzyme monomer
Metallosphaera sedula
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40000
-
2 * 40000, SDS-PAGE
Metallosphaera sedula
74000
-
gel filtration
Metallosphaera sedula
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinate semialdehyde + NADPH + H+
Metallosphaera sedula
the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea
4-hydroxybutanoate + NADP+
-
-
?
succinate semialdehyde + NADPH + H+
Metallosphaera sedula DSM 5348
the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea
4-hydroxybutanoate + NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Metallosphaera sedula
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culture condition:autotrophically-grown cell
no activity in heterotrophically-grown cell
Metallosphaera sedula
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.15
-
cell extract, NADH-dependent reaction, 65°C, pH 7.9
Metallosphaera sedula
1.4
-
cell extract, NADPH-dependent reaction, 65°C, pH 7.9
Metallosphaera sedula
700
-
purified enzyme, NADPH-dependent reaction, 65°C, pH 7.9
Metallosphaera sedula
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
malonic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde do not serve as a substrate
698628
Metallosphaera sedula
?
-
-
-
-
additional information
malonic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde do not serve as a substrate
698628
Metallosphaera sedula DSM 5348
?
-
-
-
-
succinate semialdehyde + NADH + H+
very low activity with NADH
698628
Metallosphaera sedula
4-hydroxybutanoate + NAD+
-
-
-
?
succinate semialdehyde + NADH + H+
very low activity with NADH
698628
Metallosphaera sedula DSM 5348
4-hydroxybutanoate + NAD+
-
-
-
?
succinate semialdehyde + NADPH + H+
-
698628
Metallosphaera sedula
4-hydroxybutanoate + NADP+
-
-
-
?
succinate semialdehyde + NADPH + H+
the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea
698628
Metallosphaera sedula
4-hydroxybutanoate + NADP+
-
-
-
?
succinate semialdehyde + NADPH + H+
-
698628
Metallosphaera sedula DSM 5348
4-hydroxybutanoate + NADP+
-
-
-
?
succinate semialdehyde + NADPH + H+
the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea
698628
Metallosphaera sedula DSM 5348
4-hydroxybutanoate + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 40000, SDS-PAGE
Metallosphaera sedula
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
90
-
-
Metallosphaera sedula
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
440
-
NADPH
65°C, pH 7.9
Metallosphaera sedula
440
-
succinate semialdehyde
65°C, pH 7.9
Metallosphaera sedula
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
at 20°C
Metallosphaera sedula
7.5
-
at 65°C
Metallosphaera sedula
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.5
8
at 65°C, half-maximal activity at pH 6.5 and at pH 8.0
Metallosphaera sedula
6.9
8.4
at 20°C, half-maximal activity at pH 6.9 and at pH 8.4
Metallosphaera sedula
General Information
General Information
Commentary
Organism
metabolism
the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea
Metallosphaera sedula
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea
Metallosphaera sedula
Other publictions for EC 1.1.1.B47
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
698628
Kockelkorn
Malonic semialdehyde reductase ...
Metallosphaera sedula, Metallosphaera sedula DSM 5348
J. Bacteriol.
191
6352-6362
2009
-
-
1
-
-
-
1
3
-
2
2
2
-
6
-
-
1
-
-
1
3
-
8
1
1
-
-
2
2
2
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
3
-
2
2
2
-
-
-
1
-
1
3
-
8
1
1
-
-
2
2
2
-
-
-
1
1
-
-
-