BRENDA - Enzyme Database show
show all sequences of 1.1.1.B4

Deracemization of aryl secondary alcohols via enantioselective oxidation and stereoselective reduction with tandem whole-cell biocatalysts

Li, Y.; Xu, J.; Xu, Y.; J. Mol. Catal. B 64, 48-52 (2010)
No PubMed abstract available

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
the enzyme can be used for stereospecific interconversion of (R)-1-phenylethanol and (S)-1-phenylethanol via the oxoform together with the (S)-specific secondary alcohol dehydrogenase using whole cells as biocatalysts that include the required cofactor regenration system, method, overview. Optically pure secondary alcohols are widely used in pharmaceuticals, flavors, agricultural chemicals and specialty materials
Microbacterium oxydans
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-1-phenylethanol + NAD+
Microbacterium oxydans
-
acetophenone + NADH + H+
-
-
r
(R)-1-phenylethanol + NAD+
Microbacterium oxydans ECU2010
-
acetophenone + NADH + H+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Microbacterium oxydans
-
-
-
Microbacterium oxydans ECU2010
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-1-phenylethanol + NAD+
-
725726
Microbacterium oxydans
acetophenone + NADH + H+
-
-
-
r
(R)-1-phenylethanol + NAD+
-
725726
Microbacterium oxydans ECU2010
acetophenone + NADH + H+
-
-
-
r
additional information
the enzyme catalyzes stereoselective oxidation of (R)-secondary alcohols to corresponding ketones
725726
Microbacterium oxydans
?
-
-
-
-
additional information
the enzyme catalyzes stereoselective oxidation of (R)-secondary alcohols to corresponding ketones
725726
Microbacterium oxydans ECU2010
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Microbacterium oxydans
NADH
-
Microbacterium oxydans
Application (protein specific)
Application
Commentary
Organism
synthesis
the enzyme can be used for stereospecific interconversion of (R)-1-phenylethanol and (S)-1-phenylethanol via the oxoform together with the (S)-specific secondary alcohol dehydrogenase using whole cells as biocatalysts that include the required cofactor regenration system, method, overview. Optically pure secondary alcohols are widely used in pharmaceuticals, flavors, agricultural chemicals and specialty materials
Microbacterium oxydans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Microbacterium oxydans
NADH
-
Microbacterium oxydans
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(R)-1-phenylethanol + NAD+
Microbacterium oxydans
-
acetophenone + NADH + H+
-
-
r
(R)-1-phenylethanol + NAD+
Microbacterium oxydans ECU2010
-
acetophenone + NADH + H+
-
-
r
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-1-phenylethanol + NAD+
-
725726
Microbacterium oxydans
acetophenone + NADH + H+
-
-
-
r
(R)-1-phenylethanol + NAD+
-
725726
Microbacterium oxydans ECU2010
acetophenone + NADH + H+
-
-
-
r
additional information
the enzyme catalyzes stereoselective oxidation of (R)-secondary alcohols to corresponding ketones
725726
Microbacterium oxydans
?
-
-
-
-
additional information
the enzyme catalyzes stereoselective oxidation of (R)-secondary alcohols to corresponding ketones
725726
Microbacterium oxydans ECU2010
?
-
-
-
-
Other publictions for EC 1.1.1.B4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Zhou
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Carboxydothermus hydrogenoformans, Carboxydothermus hydrogenoformans DSM 6008
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1
1
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1
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1
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1
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Acta Crystallogr. Sect. F
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1
1
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1
1
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1
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1
1
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725726
Li
-
Deracemization of aryl seconda ...
Microbacterium oxydans, Microbacterium oxydans ECU2010
J. Mol. Catal. B
64
48-52
2010
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1
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697267
Pal
Activity of yeast alcohol dehy ...
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Chem. Biol. Interact.
178
16-23
2009
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3
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697839
Pennacchio
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13
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1
1
1
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4
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711860
Machielsen
-
Cofactor engineering of Lactob ...
Lactobacillus brevis
Eng. Life Sci.
9
38-44
2009
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1
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5
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4
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4
4
684583
Pennacchio
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Thermus thermophilus
Appl. Environ. Microbiol.
74
3949-3958
2008
1
1
1
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7
5
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5
4
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1
1
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1
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7
1
2
1
4
4
2
2
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3
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1
1
1
3
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7
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5
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5
4
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1
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7
1
2
1
4
4
2
2
-
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688747
Nie
A novel NADH-dependent carbony ...
Candida parapsilosis
Lett. Appl. Microbiol.
44
555-562
2007
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9
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1
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1
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10
1
1
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1
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687864
Isobe
Thermostable NAD+-dependent (R ...
Burkholderia sp., Burkholderia sp. AIU 652
J. Biosci. Bioeng.
96
387-393
2003
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6
15
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24
1
1
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6
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15
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24
1
1
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3
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1
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688571
Bradshaw
-
A Pseudomonas sp. alcohol dehy ...
Pseudomonas sp., Pseudomonas sp. PED
J. Org. Chem.
57
1526-1532
1992
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