BRENDA - Enzyme Database show
show all sequences of 1.1.1.B4

A novel NADH-dependent carbonyl reductase with unusual stereoselectivity for (R)-specific reduction from an (S)-1-phenyl-1,2-ethanediol-producing micro-organism: purification and characterization

Nie, Y.; Xu, Y.; Yang, M.; Mu, X.Q.; Lett. Appl. Microbiol. 44, 555-562 (2007)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CaCl2
1 mM, 21% residual activity
Candida parapsilosis
CoCl2
1 mM, 57% residual activity
Candida parapsilosis
CuSO4
1 mM, complete loss of activity
Candida parapsilosis
EDTA
1 mM, 68% residual activity
Candida parapsilosis
FeCl3
1 mM, 65% residual activity
Candida parapsilosis
FeSO4
1 mM, complete loss of activity
Candida parapsilosis
MgSO4
1 mM, 94% residual activity
Candida parapsilosis
MnSO4
1 mM, 69% residual activity
Candida parapsilosis
NiCl2
1 mM, 96% residual activity
Candida parapsilosis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Candida parapsilosis
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
ZnSO4
1mM, 135% of initial activity
Candida parapsilosis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
gel filtration
Candida parapsilosis
37500
-
x * 37500, SDS-PAGE
Candida parapsilosis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Candida parapsilosis
-
-
-
Purification (Commentary)
Commentary
Organism
-
Candida parapsilosis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
14
-
pH 6.0, 30C
Candida parapsilosis
Storage Stability
Storage Stability
Organism
4C, pH 6.0-8.0, 24 h, more than 90% residual activity
Candida parapsilosis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-butanone + NAD+
274% of the activity with beta-hydroxyacetophenone
688747
Candida parapsilosis
2-butanol + NADH + H+
-
-
-
?
2-heptanone + NAD+
281% of the activity with beta-hydroxyacetophenone
688747
Candida parapsilosis
2-heptanol + NADH + H+
-
-
-
?
2-hexanone + NAD+
259% of the activity with beta-hydroxyacetophenone
688747
Candida parapsilosis
2-hexanol + NADH + H+
-
-
-
?
2-octanone + NAD+
350% of the activity with beta-hydroxyacetophenone
688747
Candida parapsilosis
2-octanol + NADH + H+
-
-
-
?
2-pentanone + NAD+
249% of the activity with beta-hydroxyacetophenone
688747
Candida parapsilosis
2-pentanol + NADH + H+
-
-
-
?
3-hexanone + NAD+
29% of the activity with beta-hydroxyacetophenone
688747
Candida parapsilosis
3-hexanol + NADH + H+
-
-
-
?
3-pentanone + NAD+
35% of the activity with beta-hydroxyacetophenone
688747
Candida parapsilosis
3-pentanol + NADH + H+
-
-
-
?
acetone + NAD+
260% of the activity with beta-hydroxyacetophenone
688747
Candida parapsilosis
isopropanol + NADH + H+
-
-
-
?
acetophenone + NADH + H+
171% of the activity with beta-hydroxyacetophenone
688747
Candida parapsilosis
phenylethanol + NAD+
-
-
-
?
beta-hydroxyacetophenone + NAD+
-
688747
Candida parapsilosis
(R)-1-phenyl-1,2-ethanediol + NADH + H+
formation of (R)-enantiomner with 100% enantiomeric excess
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
x * 37500, SDS-PAGE
Candida parapsilosis
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
-
Candida parapsilosis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Candida parapsilosis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Candida parapsilosis
NADH
-
Candida parapsilosis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Candida parapsilosis
NADH
-
Candida parapsilosis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CaCl2
1 mM, 21% residual activity
Candida parapsilosis
CoCl2
1 mM, 57% residual activity
Candida parapsilosis
CuSO4
1 mM, complete loss of activity
Candida parapsilosis
EDTA
1 mM, 68% residual activity
Candida parapsilosis
FeCl3
1 mM, 65% residual activity
Candida parapsilosis
FeSO4
1 mM, complete loss of activity
Candida parapsilosis
MgSO4
1 mM, 94% residual activity
Candida parapsilosis
MnSO4
1 mM, 69% residual activity
Candida parapsilosis
NiCl2
1 mM, 96% residual activity
Candida parapsilosis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
soluble
-
Candida parapsilosis
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
ZnSO4
1mM, 135% of initial activity
Candida parapsilosis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
gel filtration
Candida parapsilosis
37500
-
x * 37500, SDS-PAGE
Candida parapsilosis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Candida parapsilosis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
14
-
pH 6.0, 30C
Candida parapsilosis
Storage Stability (protein specific)
Storage Stability
Organism
4C, pH 6.0-8.0, 24 h, more than 90% residual activity
Candida parapsilosis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-butanone + NAD+
274% of the activity with beta-hydroxyacetophenone
688747
Candida parapsilosis
2-butanol + NADH + H+
-
-
-
?
2-heptanone + NAD+
281% of the activity with beta-hydroxyacetophenone
688747
Candida parapsilosis
2-heptanol + NADH + H+
-
-
-
?
2-hexanone + NAD+
259% of the activity with beta-hydroxyacetophenone
688747
Candida parapsilosis
2-hexanol + NADH + H+
-
-
-
?
2-octanone + NAD+
350% of the activity with beta-hydroxyacetophenone
688747
Candida parapsilosis
2-octanol + NADH + H+
-
-
-
?
2-pentanone + NAD+
249% of the activity with beta-hydroxyacetophenone
688747
Candida parapsilosis
2-pentanol + NADH + H+
-
-
-
?
3-hexanone + NAD+
29% of the activity with beta-hydroxyacetophenone
688747
Candida parapsilosis
3-hexanol + NADH + H+
-
-
-
?
3-pentanone + NAD+
35% of the activity with beta-hydroxyacetophenone
688747
Candida parapsilosis
3-pentanol + NADH + H+
-
-
-
?
acetone + NAD+
260% of the activity with beta-hydroxyacetophenone
688747
Candida parapsilosis
isopropanol + NADH + H+
-
-
-
?
acetophenone + NADH + H+
171% of the activity with beta-hydroxyacetophenone
688747
Candida parapsilosis
phenylethanol + NAD+
-
-
-
?
beta-hydroxyacetophenone + NAD+
-
688747
Candida parapsilosis
(R)-1-phenyl-1,2-ethanediol + NADH + H+
formation of (R)-enantiomner with 100% enantiomeric excess
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
x * 37500, SDS-PAGE
Candida parapsilosis
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
45
-
-
Candida parapsilosis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Candida parapsilosis
Other publictions for EC 1.1.1.B4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721911
Zhou
Biocatalytic characterization ...
Carboxydothermus hydrogenoformans, Carboxydothermus hydrogenoformans DSM 6008
Biotechnol. Lett.
35
359-365
2013
-
1
1
-
-
-
-
1
-
-
1
-
4
2
-
-
-
-
-
-
-
-
15
1
1
-
-
1
1
-
-
2
-
-
-
-
1
1
2
-
-
-
-
-
-
1
-
-
1
-
4
-
-
-
-
-
-
-
15
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
723841
Protsko
Crystallization and preliminar ...
Thermoanaerobacter ethanolicus
Acta Crystallogr. Sect. F
66
831-833
2010
-
-
1
1
1
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
1
1
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725726
Li
-
Deracemization of aryl seconda ...
Microbacterium oxydans, Microbacterium oxydans ECU2010
J. Mol. Catal. B
64
48-52
2010
-
1
-
-
-
-
-
-
-
-
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2
-
2
-
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-
-
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4
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2
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1
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2
-
-
-
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-
2
-
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-
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-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697267
Pal
Activity of yeast alcohol dehy ...
Saccharomyces carlsbergensis, Saccharomyces carlsbergensis Y379-50, Saccharomyces cerevisiae
Chem. Biol. Interact.
178
16-23
2009
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
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-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697839
Pennacchio
Role of Tryptophan 95 in subst ...
Sulfolobus solfataricus
Extremophiles
13
751-761
2009
-
-
1
1
1
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
4
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
711860
Machielsen
-
Cofactor engineering of Lactob ...
Lactobacillus brevis
Eng. Life Sci.
9
38-44
2009
-
-
1
-
5
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
2
-
2
-
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
2
-
-
-
-
-
-
-
4
4
684583
Pennacchio
Purification and characterizat ...
Thermus thermophilus
Appl. Environ. Microbiol.
74
3949-3958
2008
1
1
1
-
-
-
7
5
-
5
4
-
1
1
-
-
1
-
-
-
-
-
7
1
2
1
4
4
2
2
-
3
-
-
-
1
1
1
3
-
-
-
-
7
-
5
-
5
4
-
1
-
-
1
-
-
-
-
7
1
2
1
4
4
2
2
-
-
-
-
-
-
-
-
688747
Nie
A novel NADH-dependent carbony ...
Candida parapsilosis
Lett. Appl. Microbiol.
44
555-562
2007
-
-
-
-
-
-
9
-
1
1
2
-
-
1
-
-
1
-
-
-
1
1
10
1
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
9
-
-
1
1
2
-
-
-
-
1
-
-
1
1
10
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
687864
Isobe
Thermostable NAD+-dependent (R ...
Burkholderia sp., Burkholderia sp. AIU 652
J. Biosci. Bioeng.
96
387-393
2003
-
-
-
-
-
-
6
15
-
-
2
-
-
3
-
-
1
-
-
-
1
-
24
1
1
-
1
-
3
-
-
3
-
1
-
-
-
-
3
-
-
-
-
6
-
15
-
-
2
-
-
-
-
1
-
-
1
-
24
1
1
-
1
-
3
-
-
1
-
-
-
-
-
-
688571
Bradshaw
-
A Pseudomonas sp. alcohol dehy ...
Pseudomonas sp., Pseudomonas sp. PED
J. Org. Chem.
57
1526-1532
1992
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
1
-
-
1
-
24
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
24
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-