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Literature summary for 1.1.1.B4 extracted from

  • Isobe, K.; Wakao, N.
    Thermostable NAD+-dependent (R)-specific secondary alcohol dehydrogenase from cholesterol-utilizing Burkholderia sp. AIU 652 (2003), J. Biosci. Bioeng., 96, 387-393.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
8-hydroxyquinoline 1 mM, 55% residual activity Burkholderia sp.
alpha,alpha'-dipyridyl 1 71, 43% residual activity Burkholderia sp.
FeCl2 1 mM, 81% residual activity Burkholderia sp.
o-phenanthroline 1 mM, 43% residual activity Burkholderia sp.
p-chloromercuribenzoate 1 mM, 4% residual activity Burkholderia sp.
ZnCl2 1 mM, 73% residual activity Burkholderia sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADH pH 6.0, 30°C Burkholderia sp.
0.04
-
2-butanone pH 6.0, 30°C Burkholderia sp.
0.045
-
(R)-2-butanol pH 8.5, 30°C Burkholderia sp.
0.065
-
2-butanol pH 8.5, 30°C Burkholderia sp.
0.065
-
acetone pH 6.0, 30°C Burkholderia sp.
0.11
-
2-propanol pH 8.5, 30°C Burkholderia sp.
0.15
-
NAD+ pH 8.5, 30°C Burkholderia sp.
0.35
-
(S)-2-butanol pH 8.5, 30°C Burkholderia sp.
0.76
-
3-Pentanol pH 8.5, 30°C Burkholderia sp.
0.84
-
2-Pentanol pH 8.5, 30°C Burkholderia sp.
4
-
1-propanol pH 8.5, 30°C Burkholderia sp.
5.4
-
(R)-1,3-butanediol pH 8.5, 30°C Burkholderia sp.
6.5
-
ethanol pH 8.5, 30°C Burkholderia sp.
10
-
1-butanol pH 8.5, 30°C Burkholderia sp.
52
-
(S)-1,3-butanediol pH 8.5, 30°C Burkholderia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
4 * 37000, SDS-PAGE Burkholderia sp.
150000
-
gel filtration Burkholderia sp.

Organism

Organism UniProt Comment Textmining
Burkholderia sp.
-
growth on cholesterol or propanol as sole carbon source
-
Burkholderia sp. AIU 652
-
growth on cholesterol or propanol as sole carbon source
-

Purification (Commentary)

Purification (Comment) Organism
-
Burkholderia sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
104
-
pH 8.5, 30°C, substrate 2-propanol Burkholderia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-1,3-butanediol + NAD+
-
Burkholderia sp. ?
-
r
(R)-2-butanol + NAD+
-
Burkholderia sp. 2-butanone + NADH + H+
-
r
(S)-1,3-butanediol + NAD+
-
Burkholderia sp. ?
-
r
1,2-butanediol + NAD+ 20% of the activity with 2-propanol Burkholderia sp. ? + NADH
-
r
1,2-propanediol + NAD+ 45% of the activity with 2-propanol Burkholderia sp. ? + NADH
-
r
1,3-butanediol + NAD+ 126% of the activity with 2-propanol Burkholderia sp. ? + NADH
-
r
1-butanol + NAD+ 11% of the activity with 2-propanol Burkholderia sp. butanal + NADH
-
r
1-butanol + NAD+ 11% of the activity with 2-propanol Burkholderia sp. AIU 652 butanal + NADH
-
r
1-propanol + NAD+ 9% of the activity with 2-propanol Burkholderia sp. propanal + NADH
-
r
2,3-butanediol + NAD+ 73% of the activity with 2-propanol Burkholderia sp. ? + NADH
-
r
2,3-butanediol + NAD+ 73% of the activity with 2-propanol Burkholderia sp. AIU 652 ? + NADH
-
r
2-butanol + NAD+ (R)-isomer is preferred over (S)-isomer Burkholderia sp. 2-butanone + NADH
-
r
2-butanone + NADH
-
Burkholderia sp. (S)-2-hydroxybutane + NAD+
-
?
2-butanone + NADH 83% of the activity with acetone Burkholderia sp. 2-butanol + NAD+
-
r
2-pentanol + NAD+ 46% of the activity with 2-propanol Burkholderia sp. 2-pentanone + NADH
-
r
2-pentanol + NAD+ 46% of the activity with 2-propanol Burkholderia sp. AIU 652 2-pentanone + NADH
-
r
2-pentanone + NADH + H+ 44% of the activity with acetone Burkholderia sp. 2-pentanol + NAD+
-
r
2-propanol + NAD+ (R)-isomer is preferred over (S)-isomer Burkholderia sp. acetone + NADH
-
r
2-propanol + NAD+ (R)-isomer is preferred over (S)-isomer Burkholderia sp. AIU 652 acetone + NADH
-
r
3-pentanol + NAD+ 23% of the activity with 2-propanol Burkholderia sp. 3-pentanone + NADH
-
r
3-pentanone + NADH 16% of the activity with acetone Burkholderia sp. 3-pentanol + NAD+
-
r
4-hydroxy-2-butanone + NADH 95% of the activity with acetone Burkholderia sp. ? + NAD+
-
r
acetone + NADH
-
Burkholderia sp. 2-propanol + NAD+
-
r
ethanol + NAD+ 4% of the activity with 2-propanol Burkholderia sp. ethanal + NADH
-
r
ethanol + NAD+ 4% of the activity with 2-propanol Burkholderia sp. AIU 652 ethanal + NADH
-
r
hydroxyacetone + NADH 92% of the activity with acetone Burkholderia sp. ? + NAD+
-
r
methylpyruvate + NADH 64% of the activity with acetone Burkholderia sp. ? + NAD+
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 37000, SDS-PAGE Burkholderia sp.

Synonyms

Synonyms Comment Organism
carbonyl reductase (NADH, specific for (S)-configuration of alcohol)
-
Burkholderia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Burkholderia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
60 min, pH 7.0, 60% residual activity Burkholderia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
reduction Burkholderia sp.
8.5
-
oxidation, phosphate buffer Burkholderia sp.
9
-
and above, oxidation, Tris buffer Burkholderia sp.

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NADP+ Burkholderia sp.
NAD+
-
Burkholderia sp.
NADH
-
Burkholderia sp.

pI Value

Organism Comment pI Value Maximum pI Value
Burkholderia sp. isoelectric focusing
-
5.4