BRENDA - Enzyme Database show
show all sequences of 1.1.1.B4

Thermostable NAD+-dependent (R)-specific secondary alcohol dehydrogenase from cholesterol-utilizing Burkholderia sp. AIU 652

Isobe, K.; Wakao, N.; J. Biosci. Bioeng. 96, 387-393 (2003)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
8-hydroxyquinoline
1 mM, 55% residual activity
Burkholderia sp.
alpha,alpha'-dipyridyl
1 71, 43% residual activity
Burkholderia sp.
FeCl2
1 mM, 81% residual activity
Burkholderia sp.
o-phenanthroline
1 mM, 43% residual activity
Burkholderia sp.
p-chloromercuribenzoate
1 mM, 4% residual activity
Burkholderia sp.
ZnCl2
1 mM, 73% residual activity
Burkholderia sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NADH
pH 6.0, 30C
Burkholderia sp.
0.04
-
2-butanone
pH 6.0, 30C
Burkholderia sp.
0.045
-
(R)-2-butanol
pH 8.5, 30C
Burkholderia sp.
0.065
-
2-butanol
pH 8.5, 30C
Burkholderia sp.
0.065
-
acetone
pH 6.0, 30C
Burkholderia sp.
0.11
-
2-propanol
pH 8.5, 30C
Burkholderia sp.
0.15
-
NAD+
pH 8.5, 30C
Burkholderia sp.
0.35
-
(S)-2-butanol
pH 8.5, 30C
Burkholderia sp.
0.76
-
3-Pentanol
pH 8.5, 30C
Burkholderia sp.
0.84
-
2-Pentanol
pH 8.5, 30C
Burkholderia sp.
4
-
1-propanol
pH 8.5, 30C
Burkholderia sp.
5.4
-
(R)-1,3-butandiol
pH 8.5, 30C
Burkholderia sp.
6.5
-
ethanol
pH 8.5, 30C
Burkholderia sp.
10
-
1-butanol
pH 8.5, 30C
Burkholderia sp.
52
-
(S)-1,3-butandiol
pH 8.5, 30C
Burkholderia sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
4 * 37000, SDS-PAGE
Burkholderia sp.
150000
-
gel filtration
Burkholderia sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Burkholderia sp.
-
strain AIU 652, growth on cholesterol or propanol as sole carbon source
-
Burkholderia sp. AIU 652
-
strain AIU 652, growth on cholesterol or propanol as sole carbon source
-
Purification (Commentary)
Commentary
Organism
-
Burkholderia sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
104
-
pH 8.5, 30C, substrate 2-propanol
Burkholderia sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-butanediol + NAD+
20% of the activity with 2-propanol
687864
Burkholderia sp.
? + NADH
-
-
-
r
1,2-propanediol + NAD+
45% of the activity with 2-propanol
687864
Burkholderia sp.
? + NADH
-
-
-
r
1,3-butanediol + NAD+
126% of the activity with 2-propanol
687864
Burkholderia sp.
? + NADH
-
-
-
r
1-butanol + NAD+
11% of the activity with 2-propanol
687864
Burkholderia sp.
butanal + NADH
-
-
-
r
1-butanol + NAD+
11% of the activity with 2-propanol
687864
Burkholderia sp. AIU 652
butanal + NADH
-
-
-
r
1-propanol + NAD+
9% of the activity with 2-propanol
687864
Burkholderia sp.
propanal + NADH
-
-
-
r
2,3-butanediol + NAD+
73% of the activity with 2-propanol
687864
Burkholderia sp.
? + NADH
-
-
-
r
2,3-butanediol + NAD+
73% of the activity with 2-propanol
687864
Burkholderia sp. AIU 652
? + NADH
-
-
-
r
2-butanol + NAD+
(R)-isomer is preferred over (S)-isomer
687864
Burkholderia sp.
2-butanone + NADH
-
-
-
r
2-butanone + NADH
-
687864
Burkholderia sp.
(S)-2-hydroxybutane + NAD+
-
-
-
-
2-butanone + NADH
83% of the activity with acetone
687864
Burkholderia sp.
2-butanol + NAD+
-
-
-
r
2-pentanol + NAD+
46% of the activity with 2-propanol
687864
Burkholderia sp.
2-pentanone + NADH
-
-
-
r
2-pentanol + NAD+
46% of the activity with 2-propanol
687864
Burkholderia sp. AIU 652
2-pentanone + NADH
-
-
-
r
2-pentanone + NADH
44% of the activity with acetone
687864
Burkholderia sp.
2-pentanol + NAD+
-
-
-
r
2-propanol + NAD+
(R)-isomer is preferred over (S)-isomer
687864
Burkholderia sp.
acetone + NADH
-
-
-
r
2-propanol + NAD+
(R)-isomer is preferred over (S)-isomer
687864
Burkholderia sp. AIU 652
acetone + NADH
-
-
-
r
3-pentanol + NAD+
23% of the activity with 2-propanol
687864
Burkholderia sp.
3-pentanone + NADH
-
-
-
r
3-pentanone + NADH
16% of the activity with acetone
687864
Burkholderia sp.
3-pentanol + NAD+
-
-
-
r
4-hydroxy-2-butanone + NADH
95% of the activity with acetone
687864
Burkholderia sp.
? + NAD+
-
-
-
r
acetone + NADH
-
687864
Burkholderia sp.
2-propanol + NAD+
-
-
-
r
ethanol + NAD+
4% of the activity with 2-propanol
687864
Burkholderia sp.
ethanal + NADH
-
-
-
r
ethanol + NAD+
4% of the activity with 2-propanol
687864
Burkholderia sp. AIU 652
ethanal + NADH
-
-
-
r
hydroxyacetone + NADH
92% of the activity with acetone
687864
Burkholderia sp.
? + NAD+
-
-
-
r
methylpyruvate + NADH
64% of the activity with acetone
687864
Burkholderia sp.
? + NAD+
-
-
-
r
Subunits
Subunits
Commentary
Organism
tetramer
4 * 37000, SDS-PAGE
Burkholderia sp.
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
70
-
-
Burkholderia sp.
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
70
-
60 min, pH 7.0, 60% residual activity
Burkholderia sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
reduction
Burkholderia sp.
8.5
-
oxidation, phosphate buffer
Burkholderia sp.
9
-
and above, oxidation, Tris buffer
Burkholderia sp.
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactor: NADP+
Burkholderia sp.
NAD+
-
Burkholderia sp.
NADH
-
Burkholderia sp.
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Burkholderia sp.
isoelectric focusing
-
5.4
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactor: NADP+
Burkholderia sp.
NAD+
-
Burkholderia sp.
NADH
-
Burkholderia sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
8-hydroxyquinoline
1 mM, 55% residual activity
Burkholderia sp.
alpha,alpha'-dipyridyl
1 71, 43% residual activity
Burkholderia sp.
FeCl2
1 mM, 81% residual activity
Burkholderia sp.
o-phenanthroline
1 mM, 43% residual activity
Burkholderia sp.
p-chloromercuribenzoate
1 mM, 4% residual activity
Burkholderia sp.
ZnCl2
1 mM, 73% residual activity
Burkholderia sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NADH
pH 6.0, 30C
Burkholderia sp.
0.04
-
2-butanone
pH 6.0, 30C
Burkholderia sp.
0.045
-
(R)-2-butanol
pH 8.5, 30C
Burkholderia sp.
0.065
-
2-butanol
pH 8.5, 30C
Burkholderia sp.
0.065
-
acetone
pH 6.0, 30C
Burkholderia sp.
0.11
-
2-propanol
pH 8.5, 30C
Burkholderia sp.
0.15
-
NAD+
pH 8.5, 30C
Burkholderia sp.
0.35
-
(S)-2-butanol
pH 8.5, 30C
Burkholderia sp.
0.76
-
3-Pentanol
pH 8.5, 30C
Burkholderia sp.
0.84
-
2-Pentanol
pH 8.5, 30C
Burkholderia sp.
4
-
1-propanol
pH 8.5, 30C
Burkholderia sp.
5.4
-
(R)-1,3-butandiol
pH 8.5, 30C
Burkholderia sp.
6.5
-
ethanol
pH 8.5, 30C
Burkholderia sp.
10
-
1-butanol
pH 8.5, 30C
Burkholderia sp.
52
-
(S)-1,3-butandiol
pH 8.5, 30C
Burkholderia sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
4 * 37000, SDS-PAGE
Burkholderia sp.
150000
-
gel filtration
Burkholderia sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Burkholderia sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
104
-
pH 8.5, 30C, substrate 2-propanol
Burkholderia sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,2-butanediol + NAD+
20% of the activity with 2-propanol
687864
Burkholderia sp.
? + NADH
-
-
-
r
1,2-propanediol + NAD+
45% of the activity with 2-propanol
687864
Burkholderia sp.
? + NADH
-
-
-
r
1,3-butanediol + NAD+
126% of the activity with 2-propanol
687864
Burkholderia sp.
? + NADH
-
-
-
r
1-butanol + NAD+
11% of the activity with 2-propanol
687864
Burkholderia sp.
butanal + NADH
-
-
-
r
1-butanol + NAD+
11% of the activity with 2-propanol
687864
Burkholderia sp. AIU 652
butanal + NADH
-
-
-
r
1-propanol + NAD+
9% of the activity with 2-propanol
687864
Burkholderia sp.
propanal + NADH
-
-
-
r
2,3-butanediol + NAD+
73% of the activity with 2-propanol
687864
Burkholderia sp.
? + NADH
-
-
-
r
2,3-butanediol + NAD+
73% of the activity with 2-propanol
687864
Burkholderia sp. AIU 652
? + NADH
-
-
-
r
2-butanol + NAD+
(R)-isomer is preferred over (S)-isomer
687864
Burkholderia sp.
2-butanone + NADH
-
-
-
r
2-butanone + NADH
-
687864
Burkholderia sp.
(S)-2-hydroxybutane + NAD+
-
-
-
-
2-butanone + NADH
83% of the activity with acetone
687864
Burkholderia sp.
2-butanol + NAD+
-
-
-
r
2-pentanol + NAD+
46% of the activity with 2-propanol
687864
Burkholderia sp.
2-pentanone + NADH
-
-
-
r
2-pentanol + NAD+
46% of the activity with 2-propanol
687864
Burkholderia sp. AIU 652
2-pentanone + NADH
-
-
-
r
2-pentanone + NADH
44% of the activity with acetone
687864
Burkholderia sp.
2-pentanol + NAD+
-
-
-
r
2-propanol + NAD+
(R)-isomer is preferred over (S)-isomer
687864
Burkholderia sp.
acetone + NADH
-
-
-
r
2-propanol + NAD+
(R)-isomer is preferred over (S)-isomer
687864
Burkholderia sp. AIU 652
acetone + NADH
-
-
-
r
3-pentanol + NAD+
23% of the activity with 2-propanol
687864
Burkholderia sp.
3-pentanone + NADH
-
-
-
r
3-pentanone + NADH
16% of the activity with acetone
687864
Burkholderia sp.
3-pentanol + NAD+
-
-
-
r
4-hydroxy-2-butanone + NADH
95% of the activity with acetone
687864
Burkholderia sp.
? + NAD+
-
-
-
r
acetone + NADH
-
687864
Burkholderia sp.
2-propanol + NAD+
-
-
-
r
ethanol + NAD+
4% of the activity with 2-propanol
687864
Burkholderia sp.
ethanal + NADH
-
-
-
r
ethanol + NAD+
4% of the activity with 2-propanol
687864
Burkholderia sp. AIU 652
ethanal + NADH
-
-
-
r
hydroxyacetone + NADH
92% of the activity with acetone
687864
Burkholderia sp.
? + NAD+
-
-
-
r
methylpyruvate + NADH
64% of the activity with acetone
687864
Burkholderia sp.
? + NAD+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 37000, SDS-PAGE
Burkholderia sp.
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
70
-
-
Burkholderia sp.
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
70
-
60 min, pH 7.0, 60% residual activity
Burkholderia sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
reduction
Burkholderia sp.
8.5
-
oxidation, phosphate buffer
Burkholderia sp.
9
-
and above, oxidation, Tris buffer
Burkholderia sp.
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Burkholderia sp.
isoelectric focusing
-
5.4
Other publictions for EC 1.1.1.B4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721911
Zhou
Biocatalytic characterization ...
Carboxydothermus hydrogenoformans, Carboxydothermus hydrogenoformans DSM 6008
Biotechnol. Lett.
35
359-365
2013
-
1
1
-
-
-
-
1
-
-
1
-
4
2
-
-
-
-
-
-
-
-
15
1
1
-
-
1
1
-
-
2
-
-
-
-
1
1
2
-
-
-
-
-
-
1
-
-
1
-
4
-
-
-
-
-
-
-
15
1
1
-
-
1
1
-
-
-
-
-
-
-
-
-
723841
Protsko
Crystallization and preliminar ...
Thermoanaerobacter ethanolicus
Acta Crystallogr. Sect. F
66
831-833
2010
-
-
1
1
1
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
1
1
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
725726
Li
-
Deracemization of aryl seconda ...
Microbacterium oxydans, Microbacterium oxydans ECU2010
J. Mol. Catal. B
64
48-52
2010
-
1
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697267
Pal
Activity of yeast alcohol dehy ...
Saccharomyces carlsbergensis, Saccharomyces carlsbergensis Y379-50, Saccharomyces cerevisiae
Chem. Biol. Interact.
178
16-23
2009
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697839
Pennacchio
Role of Tryptophan 95 in subst ...
Sulfolobus solfataricus
Extremophiles
13
751-761
2009
-
-
1
1
1
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
4
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
711860
Machielsen
-
Cofactor engineering of Lactob ...
Lactobacillus brevis
Eng. Life Sci.
9
38-44
2009
-
-
1
-
5
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
2
-
2
-
-
1
-
-
-
-
-
1
1
-
5
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
2
-
2
-
-
-
-
-
-
-
4
4
684583
Pennacchio
Purification and characterizat ...
Thermus thermophilus
Appl. Environ. Microbiol.
74
3949-3958
2008
1
1
1
-
-
-
7
5
-
5
4
-
1
1
-
-
1
-
-
-
-
-
7
1
2
1
4
4
2
2
-
3
-
-
-
1
1
1
3
-
-
-
-
7
-
5
-
5
4
-
1
-
-
1
-
-
-
-
7
1
2
1
4
4
2
2
-
-
-
-
-
-
-
-
688747
Nie
A novel NADH-dependent carbony ...
Candida parapsilosis
Lett. Appl. Microbiol.
44
555-562
2007
-
-
-
-
-
-
9
-
1
1
2
-
-
1
-
-
1
-
-
-
1
1
10
1
1
-
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10
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24
1
1
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3
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3
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3
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6
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15
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24
1
1
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