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show all sequences of 1.1.1.B38

Dual coenzyme specificity of Archaeoglobus fulgidus HMG-CoA reductase

Kim, D.Y.; Stauffacher, C.V.; Rodwell, V.W.; Protein Sci. 9, 1226-1234 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Archaeoglobus fulgidus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Mevinolin
competitive
Archaeoglobus fulgidus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.16
-
NADH
pH 9.0, 50C
Archaeoglobus fulgidus
0.5
-
NAD+
pH 10.0, 50C
Archaeoglobus fulgidus
0.5
-
NADPH
pH 6.0, 50C
Archaeoglobus fulgidus
0.62
-
mevalonate
50C, pH not specified in the publication
Archaeoglobus fulgidus
1.7
-
NADP+
pH 7.0, 50C
Archaeoglobus fulgidus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Archaeoglobus fulgidus
O28538
-
-
Purification (Commentary)
Commentary
Organism
-
Archaeoglobus fulgidus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
mevalonate + CoA + 2 NAD+
optimal activity using NADP(H) occurs at pH from 1 to 3 units more acidic than that observed using NAD(H)
723732
Archaeoglobus fulgidus
3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+
-
-
-
r
mevalonate + CoA + 2 NADP+
slight preference for NAD(H) versus NADP(H). Optimal activity using NADP(H) occurs at a pH from 1 to 3 units more acidic than that observed using NAD(H)
723732
Archaeoglobus fulgidus
3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
-
-
r
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
85
-
-
Archaeoglobus fulgidus
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
65
95
65C: about 45% of maximal activity, 95C: about 65% of maximal activity
Archaeoglobus fulgidus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.08
-
NADH
pH 9.0, 50C
Archaeoglobus fulgidus
0.1
-
NAD+
pH 10.0, 50C
Archaeoglobus fulgidus
2
-
NADPH
pH 6.0, 50C
Archaeoglobus fulgidus
6.8
-
NADP+
pH 7.0, 50C
Archaeoglobus fulgidus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reductive deacylation of 3-hydroxy-3-methylglutaryl-CoA to mevalonate with NADPH
Archaeoglobus fulgidus
6.8
-
oxidative acylation of mevalonate to 3-hydroxy-3-methylglutaryl-CoA with NADP+
Archaeoglobus fulgidus
9
-
reductive deacylation of 3-hydroxy-3-methylglutaryl-CoA to mevalonate with NADH
Archaeoglobus fulgidus
9.5
10
oxidative acylation of mevalonate to 3-hydroxy-3-methylglutaryl-CoA with NAD+
Archaeoglobus fulgidus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
slight preference for NAD(H) versus NADP(H). Optimal activity using NADP(H) occurrs at a pH from 1 to 3 units more acidic than that observed using NAD(H). Each coenzyme acts as a competitive inhibitor of the other
Archaeoglobus fulgidus
NADH
slight preference for NAD(H) versus NADP(H). Optimal activity using NADP(H) occurrs at a pH from 1 to 3 units more acidic than that observed using NAD(H). Each coenzyme acts as a competitive inhibitor of the other
Archaeoglobus fulgidus
NADP+
slight preference for NAD(H) versus NADP(H). Optimal activity using NADP(H) occurrs at a pH from 1 to 3 units more acidic than that observed using NAD(H). Each coenzyme acts as a competitive inhibitor of the other
Archaeoglobus fulgidus
NADPH
slight preference for NAD(H) versus NADP(H). Optimal activity using NADP(H) occurrs at a pH from 1 to 3 units more acidic than that observed using NAD(H). Each coenzyme acts as a competitive inhibitor of the other
Archaeoglobus fulgidus
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.18
-
50C, pH not specified in the publication
Archaeoglobus fulgidus
Mevinolin
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Archaeoglobus fulgidus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
slight preference for NAD(H) versus NADP(H). Optimal activity using NADP(H) occurrs at a pH from 1 to 3 units more acidic than that observed using NAD(H). Each coenzyme acts as a competitive inhibitor of the other
Archaeoglobus fulgidus
NADH
slight preference for NAD(H) versus NADP(H). Optimal activity using NADP(H) occurrs at a pH from 1 to 3 units more acidic than that observed using NAD(H). Each coenzyme acts as a competitive inhibitor of the other
Archaeoglobus fulgidus
NADP+
slight preference for NAD(H) versus NADP(H). Optimal activity using NADP(H) occurrs at a pH from 1 to 3 units more acidic than that observed using NAD(H). Each coenzyme acts as a competitive inhibitor of the other
Archaeoglobus fulgidus
NADPH
slight preference for NAD(H) versus NADP(H). Optimal activity using NADP(H) occurrs at a pH from 1 to 3 units more acidic than that observed using NAD(H). Each coenzyme acts as a competitive inhibitor of the other
Archaeoglobus fulgidus
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.18
-
50C, pH not specified in the publication
Archaeoglobus fulgidus
Mevinolin
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Mevinolin
competitive
Archaeoglobus fulgidus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.16
-
NADH
pH 9.0, 50C
Archaeoglobus fulgidus
0.5
-
NAD+
pH 10.0, 50C
Archaeoglobus fulgidus
0.5
-
NADPH
pH 6.0, 50C
Archaeoglobus fulgidus
0.62
-
mevalonate
50C, pH not specified in the publication
Archaeoglobus fulgidus
1.7
-
NADP+
pH 7.0, 50C
Archaeoglobus fulgidus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Archaeoglobus fulgidus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
mevalonate + CoA + 2 NAD+
optimal activity using NADP(H) occurs at pH from 1 to 3 units more acidic than that observed using NAD(H)
723732
Archaeoglobus fulgidus
3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+
-
-
-
r
mevalonate + CoA + 2 NADP+
slight preference for NAD(H) versus NADP(H). Optimal activity using NADP(H) occurs at a pH from 1 to 3 units more acidic than that observed using NAD(H)
723732
Archaeoglobus fulgidus
3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+
-
-
-
r
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
85
-
-
Archaeoglobus fulgidus
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
65
95
65C: about 45% of maximal activity, 95C: about 65% of maximal activity
Archaeoglobus fulgidus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.08
-
NADH
pH 9.0, 50C
Archaeoglobus fulgidus
0.1
-
NAD+
pH 10.0, 50C
Archaeoglobus fulgidus
2
-
NADPH
pH 6.0, 50C
Archaeoglobus fulgidus
6.8
-
NADP+
pH 7.0, 50C
Archaeoglobus fulgidus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reductive deacylation of 3-hydroxy-3-methylglutaryl-CoA to mevalonate with NADPH
Archaeoglobus fulgidus
6.8
-
oxidative acylation of mevalonate to 3-hydroxy-3-methylglutaryl-CoA with NADP+
Archaeoglobus fulgidus
9
-
reductive deacylation of 3-hydroxy-3-methylglutaryl-CoA to mevalonate with NADH
Archaeoglobus fulgidus
9.5
10
oxidative acylation of mevalonate to 3-hydroxy-3-methylglutaryl-CoA with NAD+
Archaeoglobus fulgidus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.25
-
NADP+
pH 7.0, 50C
Archaeoglobus fulgidus
1
-
NADPH
pH 6.0, 50C
Archaeoglobus fulgidus
2
-
NADH
pH 9.0, 50C
Archaeoglobus fulgidus
5
-
NAD+
pH 10.0, 50C
Archaeoglobus fulgidus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.25
-
NADP+
pH 7.0, 50C
Archaeoglobus fulgidus
1
-
NADPH
pH 6.0, 50C
Archaeoglobus fulgidus
2
-
NADH
pH 9.0, 50C
Archaeoglobus fulgidus
5
-
NAD+
pH 10.0, 50C
Archaeoglobus fulgidus
Other publictions for EC 1.1.1.B38
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723732
Kim
Dual coenzyme specificity of A ...
Archaeoglobus fulgidus
Protein Sci.
9
1226-1234
2000
-
-
1
-
-
-
1
5
-
-
-
-
-
4
-
-
1
-
-
-
-
-
2
-
1
1
-
4
4
-
-
4
-
-
1
-
-
1
4
-
-
-
1
1
-
5
-
-
-
-
-
-
-
1
-
-
-
-
2
-
1
1
-
4
4
-
-
-
-
-
-
-
4
4