Application | Comment | Organism |
---|---|---|
synthesis | the immobilized enzyme is utilized in the asymmetric reduction of acetophenone to produce (S)-1-phenylethanol, with an enantiomeric excess of more than 99% | Thermus thermophilus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3) | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | covalent immobilization of the purified recombinant enzyme on different supports, i.e. on glyoxyl agarose, amino epoxy agarose, CNBr-activated sepharose, monoaminoethyl-N-ethylagarose-glutaraldehyde, monoaminoethyl-N-ethyl agarose, or polyethyleneimine agarose, immobilized enzyme activities, overview | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
recombinant enzyme, gel filtration | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-1-phenylethanol + NAD+ | Thermus thermophilus | - |
acetophenone + NADH + H+ | - |
r | |
(S)-1-phenylethanol + NAD+ | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
acetophenone + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by heat treatment at 70°C and/or adsorption chromatography on an IDA-Cu2+ support, both methods combined result in 8.8fold purification | Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.5 | - |
immobilized recombinant enzyme | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-1-phenylethanol + NAD+ | - |
Thermus thermophilus | acetophenone + NADH + H+ | - |
r | |
(S)-1-phenylethanol + NAD+ | asymmetric reduction of acetophenone to produce (S)-1-phenylethanol, with an enantiomeric excess of more than 99% | Thermus thermophilus | acetophenone + NADH + H+ | - |
r | |
(S)-1-phenylethanol + NAD+ | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | acetophenone + NADH + H+ | - |
r | |
(S)-1-phenylethanol + NAD+ | asymmetric reduction of acetophenone to produce (S)-1-phenylethanol, with an enantiomeric excess of more than 99% | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | acetophenone + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the short-chain dehydrogenases/reductase superfamily | Thermus thermophilus |
TtADH | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
73 | - |
- |
Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the thermal stability of the enzyme adsorbed by ionic exchange is very similar to the stability of the covalently immobilized enzymes in the pH range 5-9, thermal inactivation kinetics, overview | Thermus thermophilus |
70 | - |
after a thermal treatment at pH 7.0 and 70°C for 1 h, the remaining TtADH activity is over 90%, while the soluble protein concentration decreases by a 85% | Thermus thermophilus |
90 | - |
half-life is 30 min | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermus thermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
after a thermal treatment at pH 7.0 and 70°C for 1 h, the remaining TtADH activity is over 90%, while the soluble protein concentration decreases by a 85% | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermus thermophilus | |
NADH | - |
Thermus thermophilus |