BRENDA - Enzyme Database show
show all sequences of 1.1.1.B3

NAD+-Dependent (S)-specific secondary alcohol dehydrogenase involved in stereoinversion of 3-pentyn-2ol catalyzed by Nocardia fusca AKU 2123

Xie, S.-X.; Ogawa, J.; Shimizu, S.; Biosci. Biotechnol. Biochem. 63, 1721-1729 (1999)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
AgNO3
2 mM, no% residual activity
Nocardia fusca AKU 2123
CdCl2
2 mM, 6.6% residual activity
Nocardia fusca AKU 2123
EDTA
2 mM, 56% residual activity
Nocardia fusca AKU 2123
HgCl2
2 mM, no residual activity
Nocardia fusca AKU 2123
iodoacetate
2 mM, 67% residual activity
Nocardia fusca AKU 2123
o-phenanthroline
2 mM, 0.4% residual activity
Nocardia fusca AKU 2123
trypaflavine
1 mM, 7.7% residual activity
Nocardia fusca AKU 2123
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.33
-
(S)-2-hexanol
30°C, pH 7.5
Nocardia fusca AKU 2123
1.6
-
(S)-3-pentyn-2-ol
30°C, pH 7.5
Nocardia fusca AKU 2123
2.5
-
2-Hexanone
30°C, pH 7.5
Nocardia fusca AKU 2123
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
4 * 39000, SDS-PAGE
Nocardia fusca AKU 2123
150000
-
gel filtration
Nocardia fusca AKU 2123
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nocardia fusca AKU 2123
-
-
-
Purification (Commentary)
Commentary
Organism
-
Nocardia fusca AKU 2123
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
41.5
-
30°C, pH 7.5
Nocardia fusca AKU 2123
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-1-phenylethanol + NAD+
0.9% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
1-phenylethanone + NADH + H+
-
-
-
r
(R)-1-phenylethanol + NAD+
21% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
1-phenylethanone + NADH + H+
-
-
-
r
(R)-2-butanol + NAD+
6% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
2-butanone + NADH + H+
-
-
-
r
(R)-2-hexanol + NAD+
30% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
2-hexanone + NADH + H+
-
-
-
r
(R)-2-pentanol + NAD+
15% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
2-pentanone + NADH + H+
-
-
-
r
(R)-3-butyn-2-ol + NAD+
0.5% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
but-3-yn-2-one + NADH + H+
-
-
-
r
(R)-3-pentyn-2-ol + NAD+
5.6% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
pent-3-yn-2-one + NADH + H+
-
-
-
r
(S)-2-butanol + NAD+
27% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
2-butanone + NADH + H+
-
-
-
r
(S)-2-hexanol + NAD+
121% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
2-hexanone + NADH + H+
-
-
-
r
(S)-2-hexanol + NAD+
reaction is stereoselective
685643
Nocardia fusca AKU 2123
2-hexanone + NADH + H+
-
-
-
r
(S)-2-pentanol + NAD+
91% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
2-pentanone + NADH + H+
-
-
-
r
(S)-3-butyn-2-ol + NAD+
6.9% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
but-3-yn-2-one + NADH + H+
-
-
-
r
(S)-3-pentyn-2-ol + NAD+
33% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
pent-3-yn-2-one + NADH + H+
-
-
-
r
1-hexanal + NADH + H+
254% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
hexan-1-ol + NAD+
-
-
-
r
2-decanone + NADH + H+
159% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
(S)-2-decanol + NAD+
reaction is stereoselective
-
-
r
2-heptanone + NADH + H+
334% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
(S)-2-heptanol + NAD+
reaction is stereoselective
-
-
r
2-hexanone + NADH + H+
225% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
(S)-2-hexanol + NAD+
reaction is stereoselective
-
-
r
2-octanone + NADH + H+
223% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
(S)-2-octanol + NAD+
reaction is stereoselective
-
-
r
6-methyl-5-hepten-2-one + NADH + H+
-
685643
Nocardia fusca AKU 2123
(S)-6-methyl-5-hepten-2-ol + NAD+
188% of the activity with (RS)-2-hexanol
-
-
r
ethyl (R)-4-chloro-3-hydroxybutanoate + NAD+
-
685643
Nocardia fusca AKU 2123
ethyl 4-chloro-3-oxobutanoate + NADH + H+
38.1% of the activity with (RS)-2-hexanol
-
-
r
ethyl 4-chloro-3-oxobutanoate + NADH + H+
-
685643
Nocardia fusca AKU 2123
ethyl (R)-4-chloro-3-hydroxybutanoate + NAD+
-
-
-
r
additional information
primary alcohols are not accepted as substrate
685643
Nocardia fusca AKU 2123
?
-
-
-
-
n-valeraldehyde + NADH + H+
84% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
? + NAD+
-
-
-
r
pyruvic acid ethyl ester + NADH + H+
225% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
? + NAD+
-
-
-
r
Subunits
Subunits
Commentary
Organism
tetramer
4 * 39000, SDS-PAGE
Nocardia fusca AKU 2123
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
oxidation reaction
Nocardia fusca AKU 2123
65
-
reduction reaction
Nocardia fusca AKU 2123
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
30 min, pH 7.0, 85% residual activity
Nocardia fusca AKU 2123
50
-
30 min, pH 7.0, 50% residual activity
Nocardia fusca AKU 2123
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
6.5
reduction reaction
Nocardia fusca AKU 2123
8.5
9.5
oxidation reaction
Nocardia fusca AKU 2123
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
9.5
30 min, 30°C, more than 90% residual activity
Nocardia fusca AKU 2123
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
NADPH cannot substitute for NADH
Nocardia fusca AKU 2123
NAD+
-
Nocardia fusca AKU 2123
NADH
-
Nocardia fusca AKU 2123
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
NADPH cannot substitute for NADH
Nocardia fusca AKU 2123
NAD+
-
Nocardia fusca AKU 2123
NADH
-
Nocardia fusca AKU 2123
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
AgNO3
2 mM, no% residual activity
Nocardia fusca AKU 2123
CdCl2
2 mM, 6.6% residual activity
Nocardia fusca AKU 2123
EDTA
2 mM, 56% residual activity
Nocardia fusca AKU 2123
HgCl2
2 mM, no residual activity
Nocardia fusca AKU 2123
iodoacetate
2 mM, 67% residual activity
Nocardia fusca AKU 2123
o-phenanthroline
2 mM, 0.4% residual activity
Nocardia fusca AKU 2123
trypaflavine
1 mM, 7.7% residual activity
Nocardia fusca AKU 2123
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.33
-
(S)-2-hexanol
30°C, pH 7.5
Nocardia fusca AKU 2123
1.6
-
(S)-3-pentyn-2-ol
30°C, pH 7.5
Nocardia fusca AKU 2123
2.5
-
2-Hexanone
30°C, pH 7.5
Nocardia fusca AKU 2123
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
4 * 39000, SDS-PAGE
Nocardia fusca AKU 2123
150000
-
gel filtration
Nocardia fusca AKU 2123
Purification (Commentary) (protein specific)
Commentary
Organism
-
Nocardia fusca AKU 2123
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
41.5
-
30°C, pH 7.5
Nocardia fusca AKU 2123
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-1-phenylethanol + NAD+
0.9% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
1-phenylethanone + NADH + H+
-
-
-
r
(R)-1-phenylethanol + NAD+
21% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
1-phenylethanone + NADH + H+
-
-
-
r
(R)-2-butanol + NAD+
6% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
2-butanone + NADH + H+
-
-
-
r
(R)-2-hexanol + NAD+
30% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
2-hexanone + NADH + H+
-
-
-
r
(R)-2-pentanol + NAD+
15% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
2-pentanone + NADH + H+
-
-
-
r
(R)-3-butyn-2-ol + NAD+
0.5% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
but-3-yn-2-one + NADH + H+
-
-
-
r
(R)-3-pentyn-2-ol + NAD+
5.6% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
pent-3-yn-2-one + NADH + H+
-
-
-
r
(S)-2-butanol + NAD+
27% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
2-butanone + NADH + H+
-
-
-
r
(S)-2-hexanol + NAD+
121% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
2-hexanone + NADH + H+
-
-
-
r
(S)-2-hexanol + NAD+
reaction is stereoselective
685643
Nocardia fusca AKU 2123
2-hexanone + NADH + H+
-
-
-
r
(S)-2-pentanol + NAD+
91% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
2-pentanone + NADH + H+
-
-
-
r
(S)-3-butyn-2-ol + NAD+
6.9% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
but-3-yn-2-one + NADH + H+
-
-
-
r
(S)-3-pentyn-2-ol + NAD+
33% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
pent-3-yn-2-one + NADH + H+
-
-
-
r
1-hexanal + NADH + H+
254% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
hexan-1-ol + NAD+
-
-
-
r
2-decanone + NADH + H+
159% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
(S)-2-decanol + NAD+
reaction is stereoselective
-
-
r
2-heptanone + NADH + H+
334% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
(S)-2-heptanol + NAD+
reaction is stereoselective
-
-
r
2-hexanone + NADH + H+
225% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
(S)-2-hexanol + NAD+
reaction is stereoselective
-
-
r
2-octanone + NADH + H+
223% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
(S)-2-octanol + NAD+
reaction is stereoselective
-
-
r
6-methyl-5-hepten-2-one + NADH + H+
-
685643
Nocardia fusca AKU 2123
(S)-6-methyl-5-hepten-2-ol + NAD+
188% of the activity with (RS)-2-hexanol
-
-
r
ethyl (R)-4-chloro-3-hydroxybutanoate + NAD+
-
685643
Nocardia fusca AKU 2123
ethyl 4-chloro-3-oxobutanoate + NADH + H+
38.1% of the activity with (RS)-2-hexanol
-
-
r
ethyl 4-chloro-3-oxobutanoate + NADH + H+
-
685643
Nocardia fusca AKU 2123
ethyl (R)-4-chloro-3-hydroxybutanoate + NAD+
-
-
-
r
additional information
primary alcohols are not accepted as substrate
685643
Nocardia fusca AKU 2123
?
-
-
-
-
n-valeraldehyde + NADH + H+
84% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
? + NAD+
-
-
-
r
pyruvic acid ethyl ester + NADH + H+
225% of the activity with (RS)-2-hexanol
685643
Nocardia fusca AKU 2123
? + NAD+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 39000, SDS-PAGE
Nocardia fusca AKU 2123
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
oxidation reaction
Nocardia fusca AKU 2123
65
-
reduction reaction
Nocardia fusca AKU 2123
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
-
30 min, pH 7.0, 85% residual activity
Nocardia fusca AKU 2123
50
-
30 min, pH 7.0, 50% residual activity
Nocardia fusca AKU 2123
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
6.5
reduction reaction
Nocardia fusca AKU 2123
8.5
9.5
oxidation reaction
Nocardia fusca AKU 2123
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
9.5
30 min, 30°C, more than 90% residual activity
Nocardia fusca AKU 2123
Other publictions for EC 1.1.1.B3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724568
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3
1
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-
-
-
3
1
-
-
1
-
-
-
1
-
5
-
1
2
-
-
1
1
2
2
-
-
-
-
1
1
2
-
-
-
-
3
-
1
-
-
-
-
3
-
-
1
-
-
1
-
5
-
1
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1
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2
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-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
725726
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Deracemization of aryl seconda ...
Rhodotorula sp., Rhodotorula sp. AS2.2241
J. Mol. Catal. B
64
48-52
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-
1
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697267
Pal
Activity of yeast alcohol dehy ...
Saccharomyces carlsbergensis, Saccharomyces carlsbergensis Y379-50, Saccharomyces cerevisiae
Chem. Biol. Interact.
178
16-23
2009
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697839
Pennacchio
Role of Tryptophan 95 in subst ...
Sulfolobus solfataricus
Extremophiles
13
751-761
2009
-
-
1
1
1
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
4
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
701031
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-
Purification, immobilization a ...
Thermus thermophilus
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44
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-
1
1
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
1
-
2
1
1
-
3
-
1
-
1
2
-
-
-
-
1
1
2
-
1
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
1
-
2
1
1
-
3
-
1
-
1
-
-
-
-
-
-
-
684583
Pennacchio
Purification and characterizat ...
Thermus thermophilus
Appl. Environ. Microbiol.
74
3949-3958
2008
1
1
1
-
-
-
7
6
-
5
4
-
1
5
-
-
1
-
-
-
1
-
7
1
2
1
4
5
2
2
-
3
-
-
-
1
1
1
3
-
-
-
-
7
-
6
-
5
4
-
1
-
-
1
-
-
1
-
7
1
2
1
4
5
2
2
-
-
-
-
-
-
-
-
689823
Soni
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Purification and characterizat ...
Candida viswanathii, Candida viswanathii MTCC 5158
Process Biochem.
42
1632-1640
2007
1
-
-
-
-
-
30
2
-
-
2
-
7
3
-
-
1
-
-
-
1
1
14
1
1
2
1
-
1
1
-
2
-
-
-
1
-
-
2
-
-
-
-
30
-
2
-
-
2
-
7
-
-
1
-
-
1
1
14
1
1
2
1
-
1
1
-
-
-
-
-
-
-
-
685078
Hoeffken
Crystal structure and enzyme k ...
uncultured bacterium, uncultured bacterium EbN1
Biochemistry
45
82-93
2006
-
-
1
1
-
-
3
3
-
-
3
-
-
2
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
3
-
3
-
-
3
-
-
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684588
Abokitse
Cloning, sequence analysis, an ...
Rhodococcus erythropolis
Appl. Microbiol. Biotechnol.
62
380-386
2003
-
-
1
-
-
-
-
-
-
1
2
-
-
1
-
-
1
-
-
-
1
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
1
-
-
1
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685641
Yamamoto
-
Synthesis of (R)-1,3-butanedio ...
Candida parapsilosis
Biosci. Biotechnol. Biochem.
4
925-927
2002
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685644
Yamamoto
Synthesis of ethyl (R)-4-chlor ...
Candida parapsilosis
Biosci. Biotechnol. Biochem.
66
481-483
2002
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1
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685643
Xie
NAD+-Dependent (S)-specific se ...
Nocardia fusca AKU 2123
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63
1721-1729
1999
-
-
-
-
-
-
7
3
-
-
2
-
-
4
-
-
1
-
-
-
1
-
24
1
2
-
2
-
-
2
1
3
-
-
-
-
-
-
3
-
-
-
-
7
-
3
-
-
2
-
-
-
-
1
-
-
1
-
24
1
2
-
2
-
-
2
1
-
-
-
-
-
-
-