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Literature summary for 1.1.1.B3 extracted from

  • Hoeffken, H.W.; Duong, M.; Friedrich, T.; Breuer, M.; Hauer, B.; Reinhardt, R.; Rabus, R.; Heider, J.
    Crystal structure and enzyme kinetics of the (S)-specific 1-phenylethanol dehydrogenase of the denitrifying bacterium strain EbN1 (2006), Biochemistry, 45, 82-93.
    View publication on PubMed
Search for more literature for 1.1.1.B3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli uncultured bacterium

Crystallization (Commentary)

Crystallization (Comment) Organism
apo-enzyme and bound to NAD+, at a resolution of 2.1 and 2.4 A, respectively. Enzyme is a tetamer with two types of hydrophobic interfaces. NAD+-binding is associated with a conformational shift of the substrate binding loop from a crystallographically unordered open to a more ordered closed form uncultured bacterium

Inhibitors

Inhibitors Comment Organism Structure
(R)-1-phenylethanol
-
 uncultured bacterium
(S)-1-phenylethanol strong substrate inhibition, 50% of maximum activity at 0.1 mM uncultured bacterium
acetophenone inhibitory above 0.02 mM, 75% of maximum activity at 1 mM uncultured bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information enzyme exhibits positive cooperativity in both forward and reverse direction uncultured bacterium
0.0055
-
 (S)-1-phenylethanol pH 7.8, 25°C uncultured bacterium
0.0113
-
 acetophenone pH 7.8, 25°C uncultured bacterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 4 * 27000, SDS-PAGE and crystallization data, enzyme is a tetramer with two types of hydrophobic interfaces uncultured bacterium
59000
-
 gel filtration uncultured bacterium
93000
-
 PAGE uncultured bacterium

Organism

Organism UniProt Comment Textmining
uncultured bacterium
-
-
-
uncultured bacterium EbN1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-1-phenylethanol + NAD+
-
 uncultured bacterium acetophenone + NADH + H+
-
 r
(S)-1-phenylethanol + NAD+
-
 uncultured bacterium EbN1 acetophenone + NADH + H+
-
 r
acetophenone + NADH + H+
-
 uncultured bacterium (S)-1-phenylethanol + NAD+
-
 r
acetophenone + NADH + H+
-
 uncultured bacterium EbN1 (S)-1-phenylethanol + NAD+
-
 r

Subunits

Subunits Comment Organism
tetramer 4 * 27000, SDS-PAGE and crystallization data, enzyme is a tetramer with two types of hydrophobic interfaces uncultured bacterium

Synonyms

Synonyms Comment Organism
(S)-1-phenylethanol dehydrogenase
-
 uncultured bacterium
PED
-
 uncultured bacterium