BRENDA - Enzyme Database show
show all sequences of 1.1.1.B3

Cloning, sequence analysis, and heterologous expression of the gene encoding a (S)-specific alcohol dehydrogenase from Rhodococcus erythropolis DSM 43297

Abokitse, k.; Hummel, w.; Appl. Microbiol. Biotechnol. 62, 380-386 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Rhodococcus erythropolis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zinc
sequence contains catalytic and structural zinc binding sites
Rhodococcus erythropolis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36026
-
x * 36026, calculated, x * 39000, SDS-PAGE
Rhodococcus erythropolis
39000
-
x * 36026, calculated, x * 39000, SDS-PAGE
Rhodococcus erythropolis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus erythropolis
Q6YBW1
strain DSM 43297
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme
Rhodococcus erythropolis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
846
-
30C, pH 6.0
Rhodococcus erythropolis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetophenone + NADH + H+
87% of the activity with phenylacetaldehyde
684588
Rhodococcus erythropolis
alpha-phenylethanol + NAD+
-
-
-
?
capronaldehyde + NADH + H+
522% of the activity with phenylacetaldehyde
684588
Rhodococcus erythropolis
n-hexanol + NAD+
-
-
-
?
p-chloro-acetophenone + NADH + H+
288% of the activity with phenylacetaldehyde
684588
Rhodococcus erythropolis
alpha-(p-chlorophenyl)ethanol + NAD+
-
-
-
?
phenylacetaldehyde + NADH + H+
-
684588
Rhodococcus erythropolis
2-phenylethanol + NAD+
-
-
-
?
valeraldehyde + NADH + H+
433% of the activity with phenylacetaldehyde
684588
Rhodococcus erythropolis
n-pentanol + NAD+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 36026, calculated, x * 39000, SDS-PAGE
Rhodococcus erythropolis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Rhodococcus erythropolis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zinc
sequence contains catalytic and structural zinc binding sites
Rhodococcus erythropolis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36026
-
x * 36026, calculated, x * 39000, SDS-PAGE
Rhodococcus erythropolis
39000
-
x * 36026, calculated, x * 39000, SDS-PAGE
Rhodococcus erythropolis
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme
Rhodococcus erythropolis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
846
-
30C, pH 6.0
Rhodococcus erythropolis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetophenone + NADH + H+
87% of the activity with phenylacetaldehyde
684588
Rhodococcus erythropolis
alpha-phenylethanol + NAD+
-
-
-
?
capronaldehyde + NADH + H+
522% of the activity with phenylacetaldehyde
684588
Rhodococcus erythropolis
n-hexanol + NAD+
-
-
-
?
p-chloro-acetophenone + NADH + H+
288% of the activity with phenylacetaldehyde
684588
Rhodococcus erythropolis
alpha-(p-chlorophenyl)ethanol + NAD+
-
-
-
?
phenylacetaldehyde + NADH + H+
-
684588
Rhodococcus erythropolis
2-phenylethanol + NAD+
-
-
-
?
valeraldehyde + NADH + H+
433% of the activity with phenylacetaldehyde
684588
Rhodococcus erythropolis
n-pentanol + NAD+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 36026, calculated, x * 39000, SDS-PAGE
Rhodococcus erythropolis
Other publictions for EC 1.1.1.B3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Cao
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Scheffersomyces stipitis
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1
1
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-
3
1
-
-
-
-
3
1
-
-
1
-
-
-
1
-
5
-
1
2
-
-
1
1
2
2
-
-
-
-
1
1
2
-
-
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-
3
-
1
-
-
-
-
3
-
-
1
-
-
1
-
5
-
1
2
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-
1
1
2
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723841
Protsko
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Thermoanaerobacter ethanolicus
Acta Crystallogr. Sect. F
66
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1
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-
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-
1
-
1
-
-
-
-
-
-
-
-
1
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-
2
-
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-
-
2
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
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-
-
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725726
Li
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Deracemization of aryl seconda ...
Rhodotorula sp., Rhodotorula sp. AS2.2241
J. Mol. Catal. B
64
48-52
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1
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697267
Pal
Activity of yeast alcohol dehy ...
Saccharomyces carlsbergensis, Saccharomyces carlsbergensis Y379-50, Saccharomyces cerevisiae
Chem. Biol. Interact.
178
16-23
2009
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
697839
Pennacchio
Role of Tryptophan 95 in subst ...
Sulfolobus solfataricus
Extremophiles
13
751-761
2009
-
-
1
1
1
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
-
4
-
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
701031
Rocha-Martin
-
Purification, immobilization a ...
Thermus thermophilus
Process Biochem.
44
1004-1012
2009
-
1
1
-
1
-
-
-
-
-
1
1
-
1
-
-
1
-
-
-
1
-
2
1
1
-
3
-
1
-
1
2
-
-
-
-
1
1
2
-
1
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
1
-
2
1
1
-
3
-
1
-
1
-
-
-
-
-
-
-
684583
Pennacchio
Purification and characterizat ...
Thermus thermophilus
Appl. Environ. Microbiol.
74
3949-3958
2008
1
1
1
-
-
-
7
6
-
5
4
-
1
5
-
-
1
-
-
-
1
-
7
1
2
1
4
5
2
2
-
3
-
-
-
1
1
1
3
-
-
-
-
7
-
6
-
5
4
-
1
-
-
1
-
-
1
-
7
1
2
1
4
5
2
2
-
-
-
-
-
-
-
-
689823
Soni
-
Purification and characterizat ...
Candida viswanathii, Candida viswanathii MTCC 5158
Process Biochem.
42
1632-1640
2007
1
-
-
-
-
-
30
2
-
-
2
-
7
3
-
-
1
-
-
-
1
1
14
1
1
2
1
-
1
1
-
2
-
-
-
1
-
-
2
-
-
-
-
30
-
2
-
-
2
-
7
-
-
1
-
-
1
1
14
1
1
2
1
-
1
1
-
-
-
-
-
-
-
-
685078
Hoeffken
Crystal structure and enzyme k ...
uncultured bacterium, uncultured bacterium EbN1
Biochemistry
45
82-93
2006
-
-
1
1
-
-
3
3
-
-
3
-
-
2
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
3
-
3
-
-
3
-
-
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684588
Abokitse
Cloning, sequence analysis, an ...
Rhodococcus erythropolis
Appl. Microbiol. Biotechnol.
62
380-386
2003
-
-
1
-
-
-
-
-
-
1
2
-
-
1
-
-
1
-
-
-
1
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
1
-
-
1
-
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685641
Yamamoto
-
Synthesis of (R)-1,3-butanedio ...
Candida parapsilosis
Biosci. Biotechnol. Biochem.
4
925-927
2002
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
1
2
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685644
Yamamoto
Synthesis of ethyl (R)-4-chlor ...
Candida parapsilosis
Biosci. Biotechnol. Biochem.
66
481-483
2002
-
1
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685643
Xie
NAD+-Dependent (S)-specific se ...
Nocardia fusca AKU 2123
Biosci. Biotechnol. Biochem.
63
1721-1729
1999
-
-
-
-
-
-
7
3
-
-
2
-
-
4
-
-
1
-
-
-
1
-
24
1
2
-
2
-
-
2
1
3
-
-
-
-
-
-
3
-
-
-
-
7
-
3
-
-
2
-
-
-
-
1
-
-
1
-
24
1
2
-
2
-
-
2
1
-
-
-
-
-
-
-