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Literature summary for 1.1.1.B3 extracted from
- Cloning, sequence analysis, and heterologous expression of the gene encoding a (S)-specific alcohol dehydrogenase from Rhodococcus erythropolis DSM 43297 (2003), Appl. Microbiol. Biotechnol., 62, 380-386.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Rhodococcus erythropolis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zinc | sequence contains catalytic and structural zinc binding sites | Rhodococcus erythropolis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36026 | - |
x * 36026, calculated, x * 39000, SDS-PAGE | Rhodococcus erythropolis |
| 39000 | - |
x * 36026, calculated, x * 39000, SDS-PAGE | Rhodococcus erythropolis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus erythropolis | Q6YBW1 | strain DSM 43297 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Rhodococcus erythropolis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 846 | - |
30°C, pH 6.0 | Rhodococcus erythropolis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetophenone + NADH + H+ | 87% of the activity with phenylacetaldehyde | Rhodococcus erythropolis | alpha-phenylethanol + NAD+ | - |
? | |
| capronaldehyde + NADH + H+ | 522% of the activity with phenylacetaldehyde | Rhodococcus erythropolis | n-hexanol + NAD+ | - |
? | |
| p-chloro-acetophenone + NADH + H+ | 288% of the activity with phenylacetaldehyde | Rhodococcus erythropolis | alpha-(p-chlorophenyl)ethanol + NAD+ | - |
? | |
| phenylacetaldehyde + NADH + H+ | - |
Rhodococcus erythropolis | 2-phenylethanol + NAD+ | - |
? | |
| valeraldehyde + NADH + H+ | 433% of the activity with phenylacetaldehyde | Rhodococcus erythropolis | n-pentanol + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 36026, calculated, x * 39000, SDS-PAGE | Rhodococcus erythropolis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carbonyl reductase (NADH, specific for (S)-configuration of alcohol) | - |
Rhodococcus erythropolis |
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