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show all sequences of 1.1.1.B28

Characterization of the dTDP-D-fucofuranose biosynthetic pathway in Escherichia coli O52

Wang, Q.; Ding, P.; Perepelov, A.V.; Xu, Y.; Wang, Y.; Knirel, Y.A.; Wang, L.; Feng, L.; Mol. Microbiol. 70, 1358-1367 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression in Escherichia coli BL21
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
Co2+
5 mM, 11% inhibition
Escherichia coli
Cu2+
5 mM, 88% inhibition
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.38
-
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
37C, pH 7.4
Escherichia coli
0.84
-
NADH
37C, pH 7.4
Escherichia coli
0.89
-
NADPH
37C, pH 7.4
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
Fcf1 is a divalent cation-independent enzyme
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40700
-
x * 40700, His6-tagged fusion protein, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dTDP-alpha-D-fucopyranose + NADP+
Escherichia coli
the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
-
-
?
dTDP-alpha-D-fucopyranose + NADP+
Escherichia coli O52
the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Escherichia coli O52
-
-
-
Purification (Commentary)
Commentary
Organism
His6-tagged fusion protein
Escherichia coli
Storage Stability
Storage Stability
Organism
-20C, in 50% glycerol, stable for at least 5 months
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-alpha-D-fucopyranose + NADH + H+
NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
723209
Escherichia coli
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NAD+
-
-
-
?
dTDP-alpha-D-fucopyranose + NADH + H+
NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
723209
Escherichia coli O52
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NAD+
-
-
-
?
dTDP-alpha-D-fucopyranose + NADP+
the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway
723209
Escherichia coli
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
-
-
-
?
dTDP-alpha-D-fucopyranose + NADP+
the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway
723209
Escherichia coli O52
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
-
-
-
?
dTDP-alpha-D-fucopyranose + NADPH + H+
NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
723209
Escherichia coli
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADP+
-
-
-
?
dTDP-alpha-D-fucopyranose + NADPH + H+
NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
723209
Escherichia coli O52
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 40700, His6-tagged fusion protein, SDS-PAGE
Escherichia coli
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
15
37
-
Escherichia coli
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
4
50
4C: 47% of maximal activity, 50C: 36% of maximal activity
Escherichia coli
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.78
-
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
37C, pH 7.4
Escherichia coli
6.76
-
NADPH
37C, pH 7.4
Escherichia coli
26.67
-
NADH
37C, pH 7.4
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
Escherichia coli
NADPH
NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Escherichia coli BL21
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
Escherichia coli
NADPH
NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Co2+
5 mM, 11% inhibition
Escherichia coli
Cu2+
5 mM, 88% inhibition
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.38
-
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
37C, pH 7.4
Escherichia coli
0.84
-
NADH
37C, pH 7.4
Escherichia coli
0.89
-
NADPH
37C, pH 7.4
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
Fcf1 is a divalent cation-independent enzyme
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40700
-
x * 40700, His6-tagged fusion protein, SDS-PAGE
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dTDP-alpha-D-fucopyranose + NADP+
Escherichia coli
the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
-
-
?
dTDP-alpha-D-fucopyranose + NADP+
Escherichia coli O52
the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
His6-tagged fusion protein
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
-20C, in 50% glycerol, stable for at least 5 months
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dTDP-alpha-D-fucopyranose + NADH + H+
NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
723209
Escherichia coli
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NAD+
-
-
-
?
dTDP-alpha-D-fucopyranose + NADH + H+
NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
723209
Escherichia coli O52
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NAD+
-
-
-
?
dTDP-alpha-D-fucopyranose + NADP+
the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway
723209
Escherichia coli
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
-
-
-
?
dTDP-alpha-D-fucopyranose + NADP+
the enzyme is involved in the dTDP-D-fucofuranose biosynthetic pathway
723209
Escherichia coli O52
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADPH + H+
-
-
-
?
dTDP-alpha-D-fucopyranose + NADPH + H+
NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
723209
Escherichia coli
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADP+
-
-
-
?
dTDP-alpha-D-fucopyranose + NADPH + H+
NADH is a better cofactor than NADPH. kcat/Km for NADH is 4.2fold higher compared to the value for NADPH
723209
Escherichia coli O52
dTDP-4-dehydro-6-deoxy-alpha-D-glucose + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 40700, His6-tagged fusion protein, SDS-PAGE
Escherichia coli
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
15
37
-
Escherichia coli
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
4
50
4C: 47% of maximal activity, 50C: 36% of maximal activity
Escherichia coli
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.78
-
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
37C, pH 7.4
Escherichia coli
6.76
-
NADPH
37C, pH 7.4
Escherichia coli
26.67
-
NADH
37C, pH 7.4
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Escherichia coli
General Information
General Information
Commentary
Organism
malfunction
mutant strains H1862 lacking fcf1. The mutant strain produces semirough lipopolysaccharide with only one O unit attached to the core-lipid A moiety while the wild type strain produces normal lipopolysaccharide. The result indicates that fcf1 is required for the synthesis of the O-antigen in Escherichia coli O52
Escherichia coli
physiological function
the enzyme is involved in the biosynthesis of dTDP-D-fucofuranose, a component of the Escherichia coli O52 O-antigen
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
malfunction
mutant strains H1862 lacking fcf1. The mutant strain produces semirough lipopolysaccharide with only one O unit attached to the core-lipid A moiety while the wild type strain produces normal lipopolysaccharide. The result indicates that fcf1 is required for the synthesis of the O-antigen in Escherichia coli O52
Escherichia coli
physiological function
the enzyme is involved in the biosynthesis of dTDP-D-fucofuranose, a component of the Escherichia coli O52 O-antigen
Escherichia coli
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
7.4
-
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
37C, pH 7.4
Escherichia coli
7.6
-
NADPH
37C, pH 7.4
Escherichia coli
31.75
-
NADH
37C, pH 7.4
Escherichia coli
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
7.4
-
dTDP-4-dehydro-6-deoxy-alpha-D-glucose
37C, pH 7.4
Escherichia coli
7.6
-
NADPH
37C, pH 7.4
Escherichia coli
31.75
-
NADH
37C, pH 7.4
Escherichia coli
Other publictions for EC 1.1.1.B28
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723209
Wang
Characterization of the dTDP-D ...
Escherichia coli, Escherichia coli O52
Mol. Microbiol.
70
1358-1367
2008
-
-
1
-
-
-
2
3
-
1
1
2
-
5
-
-
1
-
-
-
-
1
6
1
1
1
-
3
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
2
-
3
-
1
1
2
-
-
-
1
-
-
-
1
6
1
1
1
-
3
1
-
-
-
-
2
2
-
3
3