Application | Comment | Organism |
---|---|---|
synthesis | discovery of the (S)-selective alcohol dehydrogenase enables a novel production process of (R)-acetoin from meso-2,3-butanediol | uncultured bacterium |
Cloned (Comment) | Organism |
---|---|
ADH-9 gene is identified from a biodiversity library, recombinant expression in Escherichia coli strain BL21/(DE3) | uncultured bacterium |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the regeneration of oxidised nicotinamide adenine dinucleotide is a key point in preparative application of dehydrogenases for the oxidative route. An electrochemical regeneration system is successfully combined with the BDH catalysed reaction. Up to 48 mM (R)-acetoin is produced in the reaction system while productivities up to 2 mM/h are reached. Possibility to apply an electrochemical system in a semi-preparative synthesis. Lyophilised recombinant ADH-9 from Escherichia coli BL21(DE3) cells is immobilized onto Amberlite FPA54 to 0.01 U per mg carrier leading to increased productivity compared to the immobilised form, method optimization | uncultured bacterium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
uncultured bacterium | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
meso-2,3-butanediol + NAD+ | uncultured bacterium | the (S)-specific ADH-9 produces (R)-acetoin by an oxidative route starting from meso-2,3-butanediol | (R)-acetoin + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
lyophilised recombinant ADH-9 from Escherichia coli BL21(DE3) cells by immobilization onto Amberlite FPA54 to 0.01 U per mg carrier | uncultured bacterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.14 | - |
crude recombinant enzyme, pH 7.0, 37°C | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
meso-2,3-butanediol + NAD+ | the (S)-specific ADH-9 produces (R)-acetoin by an oxidative route starting from meso-2,3-butanediol | uncultured bacterium | (R)-acetoin + NADH + H+ | - |
r | |
meso-2,3-butanediol + NAD+ | enantioselective enzymatic synthesis of the alpha-hydroxy ketone(R)-acetoin from meso-2,3-butanediol | uncultured bacterium | (R)-acetoin + NADH + H+ | - |
r | |
additional information | reaction component identification and quantification by GC-MS. No or very poor activity with isopropanol 2-methyl-1-butanol, 2-methyl-2-butanol, 2,5-hexanediol, 1,4-butanediol | uncultured bacterium | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADH-9 | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | uncultured bacterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
uncultured bacterium | |
NADH | - |
uncultured bacterium |